Gene identification and functional characterization for metabolism-based herbicide resistance in Lolium rigidum. Evolution of multiple herbicide resistance is widespread in Lolium rigidum in Australia. This resistance is very often endowed by enhanced rates of herbicide metabolism (metabolic resistance) involving cytochrome P450. This project aims to identify, clone and characterise important herbicide-metabolising P450 and other genes from multiple herbicide-resistant L. rigidum biotypes, and d ....Gene identification and functional characterization for metabolism-based herbicide resistance in Lolium rigidum. Evolution of multiple herbicide resistance is widespread in Lolium rigidum in Australia. This resistance is very often endowed by enhanced rates of herbicide metabolism (metabolic resistance) involving cytochrome P450. This project aims to identify, clone and characterise important herbicide-metabolising P450 and other genes from multiple herbicide-resistant L. rigidum biotypes, and develop transcriptional and biochemical markers for metabolic resistance diagnosis. Herbicide-metabolising gene discovery, characterisation and marker development will greatly extend the currently limited knowledge and understanding of metabolic resistance and help achieve sustainable weed management.Read moreRead less
Revealing novel mechanisms conferring evolution of resistance to glufosinate and glyphosate in Eleusine indica. Glyphosate and its alternative glufosinate are the most important herbicides in world agriculture. The world’s first cases of glufosinate resistance in Eleusine indica have been recently reported. The aims of the proposed research is to identify the currently unknown biochemical and molecular mechanisms conferring glufosinate resistance, to unravel the novel molecular mechanism endowin ....Revealing novel mechanisms conferring evolution of resistance to glufosinate and glyphosate in Eleusine indica. Glyphosate and its alternative glufosinate are the most important herbicides in world agriculture. The world’s first cases of glufosinate resistance in Eleusine indica have been recently reported. The aims of the proposed research is to identify the currently unknown biochemical and molecular mechanisms conferring glufosinate resistance, to unravel the novel molecular mechanism endowing very high level glyphosate resistance, and to elucidate the evolutionary trajectory of glyphosate resistance in E. indica. This will advance our current knowledge and understanding of resistance evolution and have impact on resistance management.Read moreRead less
Building better Brassicas: Understanding disease resistance mechanisms across the Brassicaceae. Brassica species are important crops producing cooking oil, vegetables and biofuel, grown in diverse environments with a high economic and export value. Blackleg disease, caused by the fungus Leptospheria maculans, is the most important disease of brassica crops world-wide. The newly available brassica genome sequence provides the resources to study the co-evolution of this plant and pathogen. This pr ....Building better Brassicas: Understanding disease resistance mechanisms across the Brassicaceae. Brassica species are important crops producing cooking oil, vegetables and biofuel, grown in diverse environments with a high economic and export value. Blackleg disease, caused by the fungus Leptospheria maculans, is the most important disease of brassica crops world-wide. The newly available brassica genome sequence provides the resources to study the co-evolution of this plant and pathogen. This project will characterise the evolution and conservation of resistance genes in wild and cultivated brassicas, using next-generation sequencing technology, to assess their potential for crop improvement. An understanding of the evolution of genes responsible for resistance will lead to improved plant protection strategies for brassica crops.Read moreRead less