Probing The Gene-Environment Interaction In Metabolic Disease
Funder
National Health and Medical Research Council
Funding Amount
$863,910.00
Summary
A major problem with modern medicine is it is based on the law of averages, assuming everybody responds in the same way to a drug or a diet. In view of the diversity between humans as a function of genetic, epigenetic and other factors this is not the case. In the clinic there is marked variation in the response to drugs commonly used to treat patients with type 2 diabetes. This proposal seeks to embrace this complexity to develop a precision medicine approach to future medical care.
Decision-making modules in protein interaction networks. This project aims to discover how cells use proteins to make decisions. This is important for all living things, which must react to stimuli to grow, adapt, defend themselves and to die. The project’s anticipated outcome is the systems-level identification of decision-making modules in an intracellular network. Its focus is on the smallest possible modules, which contain a decision-making protein with two modifications that control protein ....Decision-making modules in protein interaction networks. This project aims to discover how cells use proteins to make decisions. This is important for all living things, which must react to stimuli to grow, adapt, defend themselves and to die. The project’s anticipated outcome is the systems-level identification of decision-making modules in an intracellular network. Its focus is on the smallest possible modules, which contain a decision-making protein with two modifications that control protein-proteins interactions. It will investigate two recurrent decision-making modules. The expected benefits of the project include new means to decipher biological complexity, and targets to modulate biosystems by genome editing or with drugs.Read moreRead less
The role and regulation of protein methylation: a study using the recently developed methylation network of yeast. Tiny changes to proteins, such as methylation, can alter the way they interact with other proteins. This project will investigate the dynamics of protein methylation during the life of the yeast cell. The project results will be of long term relevance to situations where we may want to stop cells dividing, such as cancer or infectious disease.
The effect of methylation and phosphorylation on ribosome function. This project aims to discover how cells regulate ribosome function and selectivity, by modifying their ribosomal proteins. This affects protein synthesis, a process which is central to the growth of all living things. Expected outcomes include new knowledge on the regulation of protein synthesis, improved techniques for the study of this process and an enhanced capacity for international collaboration. New avenues for the artifi ....The effect of methylation and phosphorylation on ribosome function. This project aims to discover how cells regulate ribosome function and selectivity, by modifying their ribosomal proteins. This affects protein synthesis, a process which is central to the growth of all living things. Expected outcomes include new knowledge on the regulation of protein synthesis, improved techniques for the study of this process and an enhanced capacity for international collaboration. New avenues for the artificial regulation of the ribosome may also emerge, relevant to synthetic biology and the engineering of industrial yeasts. The project should provide significant new findings for the research community, generate research citations and contribute to a highly skilled workforce by the training of staff and students.Read moreRead less
Does phosphorylation regulate the methylation of proteins? . The interaction of proteins is a fundamental requirement of life. Tiny switches on proteins affect how they interact but little is known about how these are controlled. This project will study the complex interplay between two types of switches; one is expected to control the other. This will provide new insights into how the cell functions.
Discovery Early Career Researcher Award - Grant ID: DE150100019
Funder
Australian Research Council
Funding Amount
$352,000.00
Summary
Protein network regulation: A systems-level analysis of methylation. The systems-level analysis of protein-protein interaction networks is a vital new framework for exploring protein complex formation. However key studies into the dynamics of these networks are being precluded by a lack of broad-scale data on interactions mediated by post-translational modifications, which are known regulators of protein-protein interactions. To solve this problem, this project aims to conduct an organism-wide s ....Protein network regulation: A systems-level analysis of methylation. The systems-level analysis of protein-protein interaction networks is a vital new framework for exploring protein complex formation. However key studies into the dynamics of these networks are being precluded by a lack of broad-scale data on interactions mediated by post-translational modifications, which are known regulators of protein-protein interactions. To solve this problem, this project aims to conduct an organism-wide survey of interactions mediated by an important class of post-translational modification, methylation, using an innovative mass spectrometry-based workflow. This project aims to produce fundamental new insights into the mechanisms underlying protein-protein interaction network dynamics, and into how protein complexes are formed.Read moreRead less
How Sweet It Is: Diagnostic Clinical And Experimental Glycoproteomics
Funder
National Health and Medical Research Council
Funding Amount
$473,477.00
Summary
Most human proteins are modified by the addition of complex sugar groups, which are important for the correct function of these key biological molecules. This fellowship will develop a suite of robust mass spectrometry glycoproteomic analytics for use in conjunction with clinical cohorts, model systems and in vitro biochemistry to investigate fundamental aspects controlling N-glycosylation in disease and translation to clinical diagnostics.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100204
Funder
Australian Research Council
Funding Amount
$550,000.00
Summary
A systems biology capability for the Ramaciotti Centre for Genomics. A systems biology capability for the Ramaciotti Centre for Genomics:
The project aims to introduce a complete systems biology capability to the Ramaciotti Centre for Genomics. This is intended to provide a complete systems biology workflow, including improved data analysis for next-generation sequencing, tissue preparation and imaging, and mass spectrometry for proteomics and metabolomics. This would enable a truly systems app ....A systems biology capability for the Ramaciotti Centre for Genomics. A systems biology capability for the Ramaciotti Centre for Genomics:
The project aims to introduce a complete systems biology capability to the Ramaciotti Centre for Genomics. This is intended to provide a complete systems biology workflow, including improved data analysis for next-generation sequencing, tissue preparation and imaging, and mass spectrometry for proteomics and metabolomics. This would enable a truly systems approach to biological problems, supporting researchers and projects that focus on microbial and mammalian metabolism, carbohydrate chemistry and synthetic biology. Read moreRead less
Blocking Human Cytomegalovirus: Targeting Host Organelle Remodelling And The Viral Assembly Complex
Funder
National Health and Medical Research Council
Funding Amount
$553,477.00
Summary
Human cytomegalovirus (HCMV) is a human pathogen that infects over 60% of adults, is a significant cause of morbidity and mortality in immuno-compromised people, and a major cause of birth defects. Fundamental knowledge gaps remain in understanding host-pathogen relationships during infection. This project will systematically define molecular events giving rise to release of virus from an infected cell, and thereby reveal novel targets to block therapeutically.
Systems Approaches To Understanding The Assembly Of Mitochondrial Machines
Funder
National Health and Medical Research Council
Funding Amount
$600,005.00
Summary
Mitochondria produce the energy for our bodies. Defects in this process cause mitochondrial disease, which affects at least 1/5000 people. Diagnosis is often inconclusive as we do not understand the function of many proteins important in mitochondrial energy production. State of the art CRISPR gene-editing tools will be coupled with advanced proteomics techniques to model different types of mitochondrial disease and identify the functions of new candidate disease genes.