Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100089
Funder
Australian Research Council
Funding Amount
$700,000.00
Summary
Super-resolution fluorescence microscopy. The prestigious journal Nature Methods named super-resolution fluorescent microscopy as the Method of the Year 2008. This recognition is justified because fluorescent imaging on the molecular scale will revolutionise biological sciences. It will literally change the way we see the smallest building blocks of life and this allows researchers to identify the function of proteins and lipids in health and disease. This breakthrough technology is currently no ....Super-resolution fluorescence microscopy. The prestigious journal Nature Methods named super-resolution fluorescent microscopy as the Method of the Year 2008. This recognition is justified because fluorescent imaging on the molecular scale will revolutionise biological sciences. It will literally change the way we see the smallest building blocks of life and this allows researchers to identify the function of proteins and lipids in health and disease. This breakthrough technology is currently not available to researchers in Australia. Super-resolution fluorescence microscopy would extend Australia's leading position in the fundamental biological sciences, bio- and nano-technologies as well as imaging and microscopy.Read moreRead less
Mitochondrial biogenesis in mammalian cells. This project aims to understand the inner workings of a molecular machine involved in mitochondrial protein biogenesis. Mitochondria are essential organelles that provide the bulk of cellular energy. Genesis of the organelle relies on the coordinated synthesis and transport of both proteins and lipids that make up the organelle. This project plans to define the architecture of the molecular machine, outline how its components function, and explore the ....Mitochondrial biogenesis in mammalian cells. This project aims to understand the inner workings of a molecular machine involved in mitochondrial protein biogenesis. Mitochondria are essential organelles that provide the bulk of cellular energy. Genesis of the organelle relies on the coordinated synthesis and transport of both proteins and lipids that make up the organelle. This project plans to define the architecture of the molecular machine, outline how its components function, and explore the relationship between proteins and lipids in mitochondrial genesis. These results are expected to provide knowledge about how mitochondrial creation is regulated.Read moreRead less
Retromer directs membrane protein trafficking within the endosome. The exposure of proteins to the extracellular environment is dependent on how the travel through the various regions of the cell. The work will lead to a richer understanding of how this process is regulated by protein complexes. These complexes act within cells to drive the formation of membrane transport tubules containing cargo molecules.
Making muscle: molecular dissection of membrane domain formation. For a muscle to contract efficiently in response to an electrical signal it requires the formation of an extensive system of hollow membranous tubules through which the signal can be propagated. This proposal addresses the molecular mechanisms involved in the formation of this tubule system in skeletal muscle. This project will develop cell biology in a whole organism rather than a cell culture system and provide a new framework f ....Making muscle: molecular dissection of membrane domain formation. For a muscle to contract efficiently in response to an electrical signal it requires the formation of an extensive system of hollow membranous tubules through which the signal can be propagated. This proposal addresses the molecular mechanisms involved in the formation of this tubule system in skeletal muscle. This project will develop cell biology in a whole organism rather than a cell culture system and provide a new framework for Australian and international cell biologists. It will generate new knowledge, train young Australian scientists, help build international collaborative networks and engage the public outside the research community.Read moreRead less
Characterisation of p14ARF intracellular trafficking pathways. Over 3500 new cases of melanoma are diagnosed in NSW each year, and one of the most important proteins involved in suppressing melanoma initiation or growth is p14ARF. This project will characterise the movement and functions of this protein with the aim of identifying novel targets for more effective drug therapies.
Discovery Early Career Researcher Award - Grant ID: DE200100611
Funder
Australian Research Council
Funding Amount
$427,116.00
Summary
How do extracellular vesicles fuse with cells to deliver messages? Aims: This project aims to investigate how tiny packages released by all cells in the human body, called extracellular vesicles, deliver messages into neighbouring cells facilitating cell-to-cell communication.
Significance: This project expects to generate key knowledge in the area of cell-to-cell communication by using innovative molecular biology approaches and cutting-edge microscopy and biophysical techniques.
Expected outco ....How do extracellular vesicles fuse with cells to deliver messages? Aims: This project aims to investigate how tiny packages released by all cells in the human body, called extracellular vesicles, deliver messages into neighbouring cells facilitating cell-to-cell communication.
Significance: This project expects to generate key knowledge in the area of cell-to-cell communication by using innovative molecular biology approaches and cutting-edge microscopy and biophysical techniques.
Expected outcomes: Expected outcomes include high resolution details of which molecules are packaged onto extracellular vesicles and how they are delivered into recipient cells.
Benefits: This project should contribute significantly to understanding extracellular vesicle function and guide their eventual use as therapeutics.Read moreRead less
Understanding how Plasmepsin V directs export of malaria virulence proteins to the host cell. This project aims to characterise how malaria parasites survive and manipulate infected host cells by exporting virulence proteins. This project may identify essential proteins that allow the malaria parasite to transform the host in order to survive, replicate and hide from the immune system and provide new data on protein export in liver-stages.
Expression and substrate recognition by MARCH ubiquitin ligases. Eukaryotic cells are compartmentalised, with different organelles playing distinct functions. This project will characterise the MARCHs, proteins which control the localisation and half-life of other proteins. Understanding how the MARCHs work will provide novel insights into fundamental cellular processes that play major roles in many biological functions.
Deciphering the cellular defences against aggregating proteins in human disease. Cells have inbuilt defences for coping with proteins that bend into abnormal sticky shapes that form toxic clusters. In many diseases, including Huntington's, the clusters severely damage nerve cells. This project will identify the genes and mechanisms cells use to protect themselves from toxic clusters, which could provide new therapeutic targets.
The molecular blue-print for a mitochondrial nanomachine. The objective of the project is to develop a comprehensive understanding of the architecture of a biological nanomachine through broad-reaching investigation of the molecular contacts that enable the component parts to work together. The project plans to take the foundation knowledge of each of the component parts and build a conceptual framework of engineering principles to understand how the nanomachine is assembled, using a breakthroug ....The molecular blue-print for a mitochondrial nanomachine. The objective of the project is to develop a comprehensive understanding of the architecture of a biological nanomachine through broad-reaching investigation of the molecular contacts that enable the component parts to work together. The project plans to take the foundation knowledge of each of the component parts and build a conceptual framework of engineering principles to understand how the nanomachine is assembled, using a breakthrough technology to address the precise architecture of the component parts within the nanomachine.Read moreRead less