MOLECULAR BREEDING OF CYTOCHROME P450 ENZYMES. Cytochrome P450s are enzymes that catalyse an impressive array of oxidative transformations. However, there is little available data on how to modify their substrate specificity and generate tailored biocatalysts. We plan to use an emerging technology known as DNA shuffling to create libraries of P450s with varying activities. These will then be screened for enzymes that can catalyse the formation of indigo (a blue dye) and indirubin (a chemother ....MOLECULAR BREEDING OF CYTOCHROME P450 ENZYMES. Cytochrome P450s are enzymes that catalyse an impressive array of oxidative transformations. However, there is little available data on how to modify their substrate specificity and generate tailored biocatalysts. We plan to use an emerging technology known as DNA shuffling to create libraries of P450s with varying activities. These will then be screened for enzymes that can catalyse the formation of indigo (a blue dye) and indirubin (a chemotherapeutic agent). The enzymes that catalyse indigo formation will be useful in the production of coloured transgenic plants and those that produce indirubin will have a role in gene therapy.Read moreRead less
Enhanced Multilingual Speaker Recognition through the Incorporation of High-Level Features, Late Fusion and Discriminative Classification Methods. The development of robust multilingual speaker recognition systems will benefit the community through the elimination of fraud incurred by financial institutions and customers by enabling several person authentication applications such as: voice based signatures and document issuance; credit card verification by voice and secure over-the-phone financi ....Enhanced Multilingual Speaker Recognition through the Incorporation of High-Level Features, Late Fusion and Discriminative Classification Methods. The development of robust multilingual speaker recognition systems will benefit the community through the elimination of fraud incurred by financial institutions and customers by enabling several person authentication applications such as: voice based signatures and document issuance; credit card verification by voice and secure over-the-phone financial transactions. The technology will also assist in the protection of the community and safeguard Australia by enabling the implementation of the following: suspect identification using voice print; national security measures for combating terrorism by using voice to locate and track terrorists; preemptive criminal activity counter-measures; surveillance and secure building access by voice.Read moreRead less
Robust speaker recognition with reduced utterance duration and intersession variability. The development of robust and accurate speaker recognition systems will enable secure person authentication in over-the-phone financial transactions and benefit the community through the elimination of identity fraud incurred by customers and financial institutions. The technology will also assist in safeguarding Australia by enabling the implementation of suspect identification using voice and security meas ....Robust speaker recognition with reduced utterance duration and intersession variability. The development of robust and accurate speaker recognition systems will enable secure person authentication in over-the-phone financial transactions and benefit the community through the elimination of identity fraud incurred by customers and financial institutions. The technology will also assist in safeguarding Australia by enabling the implementation of suspect identification using voice and security measures for combating terrorism by using voice to locate and track terrorists. Our research at QUT Speech Research Lab is at the forefront of development in this field and will provide Australia with a technological advantage in the rapidly evolving global market for speaker recognition technology for person authentication applications.Read moreRead less
Techniques to use stereo vision for improving person identification systems based on face recognition. The broad aim of this project is to use three-dimensional information available by processing images from stereo cameras in order to bridge the gap between constrained face recognition systems and viable systems that work well under varying illumination, changes in pose and variations in spectacles, facial hair and attire. Such a system will be useful in passenger verification at airports and a ....Techniques to use stereo vision for improving person identification systems based on face recognition. The broad aim of this project is to use three-dimensional information available by processing images from stereo cameras in order to bridge the gap between constrained face recognition systems and viable systems that work well under varying illumination, changes in pose and variations in spectacles, facial hair and attire. Such a system will be useful in passenger verification at airports and as a component of personal identification systems to counter terrorism. The key to successful face location and recognition is an effective combination of all data - range, luminance and colour - and techniques for this will be the discovered outcomes.Read moreRead less
Organophosphate pesticide degradation: evolved enzymes and biomimetics for bioremediation and medicine. Organophosphate (OP) pesticides are an indispensable part of modern agriculture - their use results in dramatically increased crop yields. However, they are toxic and can damage the environment and cause significant health problems. Enzymes are currently being used to treat runoff water that is contaminated with OPs. The same enzymes also have the potential to aid in the treatment of OP poison ....Organophosphate pesticide degradation: evolved enzymes and biomimetics for bioremediation and medicine. Organophosphate (OP) pesticides are an indispensable part of modern agriculture - their use results in dramatically increased crop yields. However, they are toxic and can damage the environment and cause significant health problems. Enzymes are currently being used to treat runoff water that is contaminated with OPs. The same enzymes also have the potential to aid in the treatment of OP poisoning. However, OP degrading enzymes could be improved in many ways - we will evolve these enzymes to enhance their catalytic properties - to enable them to act more efficiently on an increased number of OPs. Read moreRead less
The molecular biology and biochemistry of bacterial manganese oxidation. This project will further the understanding of bacterial manganese (Mn2+) oxidation. A multi-disciplinary approach will be used to further investigate the genetics and biochemistry of the Mn2+-oxidising systems of Pseudomonas putida, Leptothrix sp. and Pedomicrobium sp. This work will focus in particular on comparing the Mn2+-oxidising systems from unrelated bacteria. A combination of molecular biology, protein biochemis ....The molecular biology and biochemistry of bacterial manganese oxidation. This project will further the understanding of bacterial manganese (Mn2+) oxidation. A multi-disciplinary approach will be used to further investigate the genetics and biochemistry of the Mn2+-oxidising systems of Pseudomonas putida, Leptothrix sp. and Pedomicrobium sp. This work will focus in particular on comparing the Mn2+-oxidising systems from unrelated bacteria. A combination of molecular biology, protein biochemistry and spectroscopy will be used. This will be the first time that the enzymes of bacterial Mn2+-oxidation will have been characterised in such detail and will lead to a greater understanding of the process of bacterial manganese oxidation.Read moreRead less
Automatic Ontology Learning and Data Reasoning in Web Mining. This research has an impact on both research and practical applications. In research, it provides opportunities for research students to carry out research using both data mining and data reasoning to solving Web based application problems. In practical, it can help IT industry to design the new generation of Web mining systems in order to provide invaluable service to users. This research also develops new techniques for data automa ....Automatic Ontology Learning and Data Reasoning in Web Mining. This research has an impact on both research and practical applications. In research, it provides opportunities for research students to carry out research using both data mining and data reasoning to solving Web based application problems. In practical, it can help IT industry to design the new generation of Web mining systems in order to provide invaluable service to users. This research also develops new techniques for data automatic processing within areas of smart information use in Australia. In particular it further develops data mining techniques by introducing data reasoning models for using discovered knowledge. It must be useful to improve the efficiency of the existing data mining systems. Read moreRead less
The protein O-glycosylation pathway of Neisseria: a model system for O-glycosylation of bacterial proteins with potential use in biotechnology. Proteins can be modified by the addition of sugar molecules. This process, called glycosylation, has been studied for some time in humans and other higher organisms, but is relatively new in the field of bacteria. This study will use the bacterium Neisseria as a model system for this process and work to harness the system for use in biotechnology.
Algal direct-air CO2 capture through interfacial enzyme immobilisation . Capturing CO2 directly from the atmosphere is challenging due to inherently slow mass transfer kinetics. This project aims to overcome this using an enzyme that can rapidly solubilise CO2 from air into water, to produce algae. By engineering the enzyme immobilisation at the air-water interface, this project will activate and protect the enzymes, increasing their lifespan and reducing costs. By understanding mass transfer an ....Algal direct-air CO2 capture through interfacial enzyme immobilisation . Capturing CO2 directly from the atmosphere is challenging due to inherently slow mass transfer kinetics. This project aims to overcome this using an enzyme that can rapidly solubilise CO2 from air into water, to produce algae. By engineering the enzyme immobilisation at the air-water interface, this project will activate and protect the enzymes, increasing their lifespan and reducing costs. By understanding mass transfer and enzyme activity in the interfacial immobilisation media, floating enzyme rafts can be developed for deployment over expansive areas, facilitating large-scale conversion of atmospheric CO2 into algae-derived fuels, feeds and chemicals.Read moreRead less
Targeted bioengineering and systems biology for solar powered hydrogen production in green algal cells. The development of clean fuels to combat climate change and protect against oil price shocks, is an urgent challenge facing our society. Fuels make up ~67% of the energy market, yet most low-CO2 emissions technologies (e.g. nuclear and clean-coal-technology) target the electricity market. In contrast the Solar Bio-H2 process uses algal photobioreactors to drive solar-powered H2 fuel production ....Targeted bioengineering and systems biology for solar powered hydrogen production in green algal cells. The development of clean fuels to combat climate change and protect against oil price shocks, is an urgent challenge facing our society. Fuels make up ~67% of the energy market, yet most low-CO2 emissions technologies (e.g. nuclear and clean-coal-technology) target the electricity market. In contrast the Solar Bio-H2 process uses algal photobioreactors to drive solar-powered H2 fuel production from water (ultimately sea water, facilitating desalination). This project aims to improve the efficiency of the process towards economical levels. The Solar Bio-H2 process reduces water requirements for biofuel production. Locating bioreactors on non-arable land also eliminates competition between biofuel and food production.Read moreRead less