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Research Topic : polymerase chain r
Scheme : NHMRC Project Grants
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  • Funded Activity

    Development Of Molecular Diagnostics For The Detection Of Donovanosis (Granuloma Inguinale)

    Funder
    National Health and Medical Research Council
    Funding Amount
    $174,028.00
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    Funded Activity

    The Characterisation And Functional Analysis Of A Novel Nkx2.5-regulated Gene In Cardiac And Skeletal Muscle.

    Funder
    National Health and Medical Research Council
    Funding Amount
    $257,793.00
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    Diseases Caused By A Recently Discovered Bacteria In So Cially Disadvantaged Aboriginal People In The Nt

    Funder
    National Health and Medical Research Council
    Funding Amount
    $211,392.00
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    Funded Activity

    Investigation Of A Novel Enzyme Regulating Platelet Fun Ction

    Funder
    National Health and Medical Research Council
    Funding Amount
    $77,735.00
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    Funded Activity

    Microbiological And Immunological Determinants Of Prolonged Illness Following Q Fever.

    Funder
    National Health and Medical Research Council
    Funding Amount
    $362,036.00
    Summary
    Q fever is a severe, sometimes life-threatening infection acquired by individuals who work with livestock, particularly abattoir workers. At least 10% of individuals who develop Q fever experience prolonged ill-health in the form of weeks or months of debilitating fatigue, profuse night sweats, headaches, as well as muscle and joint pains. This poorly understood persistent illness is associated with substantial disability and loss of income. This research is based upon an established cohort stud .... Q fever is a severe, sometimes life-threatening infection acquired by individuals who work with livestock, particularly abattoir workers. At least 10% of individuals who develop Q fever experience prolonged ill-health in the form of weeks or months of debilitating fatigue, profuse night sweats, headaches, as well as muscle and joint pains. This poorly understood persistent illness is associated with substantial disability and loss of income. This research is based upon an established cohort study in which subjects with acute, documented Q fever are recruited shortly after the onset of symptoms and followed at regular intervals through to recovery or persistent symptoms. The aim of this research is to determine whether abnormal persistence of the causative organsim of Q fever, Coxiella burnetii, underlies the continued symptoms in those who do not recover promptly from the acute illness. Furthermore, the research is examining the host defense response against the organism via the production of cytokines or immunological hormones, to determine whether these proteins mediate the ongoing symptoms. If confirmed, these hypotheses would lead the way to diagnostic markers for the disorder and a rational treatment strategy.
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    Funded Activity

    Sensitive Measurement Of The Number Of Leukaemic Cells

    Funder
    National Health and Medical Research Council
    Funding Amount
    $277,509.00
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    Funded Activity

    A New Virus Causing Acute Gastroenteritis In Humans

    Funder
    National Health and Medical Research Council
    Funding Amount
    $575,374.00
    Summary
    Diarrhoea is very common, especially in children but a cause is often not found. Believing there must be undiscovered viruses responsible, we developed a new method to look for them, and discovered one, which we have named adelavirus, in 17% of children with diarrhoea presenting to the WCH, Adelaide, over a 3 month period. 55% were hospitalised. This project proposes to investigate how widespread adelavirus infection is in the community and investigate how a vaccine might be developed.
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    Funded Activity

    MINIMAL RESIDUAL DISEASE IN ACUTE LYMPHOBLASTIC LEUKAEMIA

    Funder
    National Health and Medical Research Council
    Funding Amount
    $455,204.00
    Summary
    This project will study the extremely small numbers of leukaemic cells which are found in patients who are apparently healthy, but which sometimes lead to relapse. Very sensitive methods for measuring and studying low levels of leukaemic cells will be developed and used. To develop new better treatments in the long term, we will study why current treatment sometimes fails to eradicate the leukaemia, leading to patients relapsing. Clinicians currently need to obtain samples of bone marrow to asse .... This project will study the extremely small numbers of leukaemic cells which are found in patients who are apparently healthy, but which sometimes lead to relapse. Very sensitive methods for measuring and studying low levels of leukaemic cells will be developed and used. To develop new better treatments in the long term, we will study why current treatment sometimes fails to eradicate the leukaemia, leading to patients relapsing. Clinicians currently need to obtain samples of bone marrow to assess leukaemia, and the research will show whether this needs to be continued, or whether, with sensitive tests, samples of blood can be used instead. The study will involve collaboration with clinicians throughout Australia and overseas.
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    Funded Activity

    Trichomonas Vaginalis: Prevalence In An Urban Indigenous Population And Validation Of New Assays

    Funder
    National Health and Medical Research Council
    Funding Amount
    $88,880.00
    Summary
    The infection called trichomonas is the commonest sexually transmitted infection (STI) world-wide. It is caused by a parasite and is particularly common amongst disadvantaged, poor communities. It is important to diagnose and treat (readily available treatments exist) this infection because it is linked with early birth in pregnant women and increases the spread of HIV-AIDS virus. The diagnosis has not changed for decades until recently, when a new more sensitive test became available.Wehave ada .... The infection called trichomonas is the commonest sexually transmitted infection (STI) world-wide. It is caused by a parasite and is particularly common amongst disadvantaged, poor communities. It is important to diagnose and treat (readily available treatments exist) this infection because it is linked with early birth in pregnant women and increases the spread of HIV-AIDS virus. The diagnosis has not changed for decades until recently, when a new more sensitive test became available.Wehave adapted it to work on self-collected specimens such as in-out tampons or urines. Proof that this new diagnostic tool is better, is needed in Australia in high-risk groups such as Aboriginal and Torres Strait Islander communities where other STIs have already been shown to be at high rates. How this parasite causes disease in humans is poorly studied. We plan to test this new diagnostic method and compare it to traditional tests in a high-risk population and to ultimately study how the parasite causes disease.
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    Funded Activity

    The Use Of Minimal Residual Disease Detection To Improve Treatment Outcome In Childhood Acute Lymphoblastic Leukaemia

    Funder
    National Health and Medical Research Council
    Funding Amount
    $316,650.00
    Summary
    Leukaemia is the most common childhood cancer, representing approximately 35% of all cases. Despite intensive therapy, the disease frequently recurs in the bone marrow and although children are classified into good and poor prognosis groups at diagnosis based on a number of criteria, relapses nevertheless occur in both groups. Available evidence suggests that early detection of poor treatment response in the otherwise good prognosis group, and the implementation of alternative therapy when the c .... Leukaemia is the most common childhood cancer, representing approximately 35% of all cases. Despite intensive therapy, the disease frequently recurs in the bone marrow and although children are classified into good and poor prognosis groups at diagnosis based on a number of criteria, relapses nevertheless occur in both groups. Available evidence suggests that early detection of poor treatment response in the otherwise good prognosis group, and the implementation of alternative therapy when the cancer burden is at a low level, has a high likelihood of improving patient survival. The failure to respond well to treatment is assessed by a novel molecular genetic technique developed in our laboratory that can detect and quantitate very low levels of residual leukaemia with great sensitivity and specificity. The major goal of this project is to conduct a clinical trial in which this testing procedure is used at an early stage of treatment, and patients who have a bad result on this test, will be given more intensive treatment to see if this improves survival rates. In addition, the project is also directed towards investigating a range of genes known to have a role in drug detoxification. A number of naturally occurring variations exist for these drug metabolising genes and there is evidence suggesting that specific variations or patterns may influence a cancer's response to treatment. We will therefore examine the genetic patterns present in a large cohort of leukaemias and correlate these patterns with response to treatment. It is anticipated that these studies will help define the most appropriate treatment strategies for children with leukaemia. This project therefore has major implications for the therapeutic management of children with leukaemia and has the potential of contributing directly to the improved survival of this most common of childhood cancers.
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