Understanding and avoiding transgene silencing in sugarcane. Sugarcane is one of the world's major crops for food (sugar) and fuel (ethanol, electricity co-generation). It is one of the most appealing target crops for metabolic engineering aimed at renewable biomaterials and biofuels. Australia has invested strongly to achieve scientific leadership in gene technologies in our major export crops including sugarcane. Field tests show that development of methods to avoid unstable expression or 'sil ....Understanding and avoiding transgene silencing in sugarcane. Sugarcane is one of the world's major crops for food (sugar) and fuel (ethanol, electricity co-generation). It is one of the most appealing target crops for metabolic engineering aimed at renewable biomaterials and biofuels. Australia has invested strongly to achieve scientific leadership in gene technologies in our major export crops including sugarcane. Field tests show that development of methods to avoid unstable expression or 'silencing' of introduced genes is now a critical requirement for practical application. The current project emerges from industry recognition of the need to understand and avoid transgene silencing. The methods developed using sugarcane are expected to have rapid applicability for wider benefits in agriculture.Read moreRead less
The structure and function of cyanobacterial carboxysome multi-protein complexes and their role in carbon sequestration in cyanobacteria. Cyanobacteria are important contributors to global photosynthesis and have evolved unique mechanisms for capturing CO2 from their aquatic environments. Understanding these molecular mechanisms is important for both predicting how cyanobacteria affect carbon fixation at the global scale, and how their genetic specialisation may be used for improving photosynthe ....The structure and function of cyanobacterial carboxysome multi-protein complexes and their role in carbon sequestration in cyanobacteria. Cyanobacteria are important contributors to global photosynthesis and have evolved unique mechanisms for capturing CO2 from their aquatic environments. Understanding these molecular mechanisms is important for both predicting how cyanobacteria affect carbon fixation at the global scale, and how their genetic specialisation may be used for improving photosynthesis in agricultural plants. This project aims to examine one particular aspect of this specialisation, the multi-protein carboxysome complex, where CO2 fixation occurs. Using recent whole-genome information we will take a proteogenomic approach to understanding the structure and function of the carboxysome and how it contributes to the photosynthesis of the cell.Read moreRead less
Nano-molecular structure and function of protein mini-compartments known as carboxysomes. Intriguing protein nano-structures, present in blue-green algae and known as carboxysomes, act as tiny compartments where CO2 can be fixed into simple sugars at high efficiency. This important photosynthetic process forms the basis of global primary productivity on this planet, but most land-based CO2 fixation lacks the efficiency seen in blue-greens. This research aims to determine how the several proteins ....Nano-molecular structure and function of protein mini-compartments known as carboxysomes. Intriguing protein nano-structures, present in blue-green algae and known as carboxysomes, act as tiny compartments where CO2 can be fixed into simple sugars at high efficiency. This important photosynthetic process forms the basis of global primary productivity on this planet, but most land-based CO2 fixation lacks the efficiency seen in blue-greens. This research aims to determine how the several proteins that make up carboxysomes come together to makeup carboxysome nano-structures and how these function to enhance rates of CO2 fixation. A more thorough understanding of the carboxysome is likely to have potential applications in industrial nano-technology and improvements in crop productivity.Read moreRead less