Directed evolution of ancestral bacterial flagellar motors. This project aims to produce new knowledge concerning the adaptation of bacterial species to wide environmental changes. The bacterial flagellar motor (BFM) is a motor 40 nanometers in diameter that builds itself into bacterial membranes, rotates five times faster than a Formula One engine, and switches directions in milliseconds. . This project will combine ancestral reconstruction of ancient motor components with protein engineering t ....Directed evolution of ancestral bacterial flagellar motors. This project aims to produce new knowledge concerning the adaptation of bacterial species to wide environmental changes. The bacterial flagellar motor (BFM) is a motor 40 nanometers in diameter that builds itself into bacterial membranes, rotates five times faster than a Formula One engine, and switches directions in milliseconds. . This project will combine ancestral reconstruction of ancient motor components with protein engineering to understand how the different ion channels that power the BFM in different species are selective for different positive ions. It will inspire and inform future manufacturing in bionanotechnology.Read moreRead less
Molecular Cell Biology and Comparative Genomics Of Planctomycetes and Verrucomicrobia In Relation To Evolution Of Cytoskeletal Proteins and Membrane-bounded Compartments. Planctomycetes and verrucomicrobia are evolutionarily distinct groups of bacteria which possess unusual cell structure and which share some significant genes important in cell biology with eukaryotes e.g. in verrucomicrobia the cytoskeleton protein tubulin. These bacteria are important for understanding the transition from no ....Molecular Cell Biology and Comparative Genomics Of Planctomycetes and Verrucomicrobia In Relation To Evolution Of Cytoskeletal Proteins and Membrane-bounded Compartments. Planctomycetes and verrucomicrobia are evolutionarily distinct groups of bacteria which possess unusual cell structure and which share some significant genes important in cell biology with eukaryotes e.g. in verrucomicrobia the cytoskeleton protein tubulin. These bacteria are important for understanding the transition from non-nucleated cells with simple cell division to nucleated cells with chromosome separation via cytoskeletal protein movement.The project will compare genomes of of planctomycetes and verrucomicrobia to determine their relationship, determine whether the tubulin homolog of verrucomicrobia can form cytoskeleton structures, and characterize the cytoskeleton of ammonium-oxidizing planctomycetes used in wastewater treatment.Read moreRead less