Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs. The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics ....Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs. The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics for use in real world applications. The project has two aspects. First, relatively small directed cDNA-bead libraries will be compared to known low-density cDNA microarrays to validate the technique for utility in gene expression profiling. Secondly, large libraries containing short oligonucleotide sequences will be used for de novo sequencing of a complete transcriptome. Proof-of-concept in either case will pave the way for many genomic applications and catapult the technology to 'blockbuster' status.Read moreRead less
Classification of Microarray Gene-Expression Data. The broad aim is to provide statistical methodology for the classification of microarray gene-expression data. Microarrays are part of a new biotechnology that allows the monitoring of expression levels for thousands of genes simultaneously. The explosion in microarrays has produced massive quantities of data that require new statistical techniques for analysis in order to exploit their enormous scientific potential. One of the main uses of ....Classification of Microarray Gene-Expression Data. The broad aim is to provide statistical methodology for the classification of microarray gene-expression data. Microarrays are part of a new biotechnology that allows the monitoring of expression levels for thousands of genes simultaneously. The explosion in microarrays has produced massive quantities of data that require new statistical techniques for analysis in order to exploit their enormous scientific potential. One of the main uses of the methodology to be developed is to expedite the discovery of new subclasses of diseases. Another is to provide prediction rules for the diagnosis and treatment of diseases.Read moreRead less
Sequencing the mammalian transcriptome in toto. The mammalian genome projects have provided a huge leap forward for biological research by giving the framework to study genes on a global scale. This project will provide human genome project scale information for every major tissue in mammals and will provide the research community with a world class resource to further genomic research. It will also investigate parts of gene regulation which have been invisible to researchers in the past. Discov ....Sequencing the mammalian transcriptome in toto. The mammalian genome projects have provided a huge leap forward for biological research by giving the framework to study genes on a global scale. This project will provide human genome project scale information for every major tissue in mammals and will provide the research community with a world class resource to further genomic research. It will also investigate parts of gene regulation which have been invisible to researchers in the past. Discovering these rules will provide novel insights in pathology with a genetic component and provide a further boost to biotechnological approaches to obtain expression of specific sets of genes in model systems. Read moreRead less
Beyond Microarrays: Nano-Scaled Devices for High Throughput Biomolecular Sensing. Current developments in Nanoscience and Nanotechnology hold many promises in terms of revolutionising our industrial base, transforming biology, medical science and practice. This project strives to achieve some of these aims by, for the first time, building and testing nano-scaled devices with the capability to 'read' massive amounts of biological information. With the recent completion of the Human Genome proje ....Beyond Microarrays: Nano-Scaled Devices for High Throughput Biomolecular Sensing. Current developments in Nanoscience and Nanotechnology hold many promises in terms of revolutionising our industrial base, transforming biology, medical science and practice. This project strives to achieve some of these aims by, for the first time, building and testing nano-scaled devices with the capability to 'read' massive amounts of biological information. With the recent completion of the Human Genome project, major opportunities exist to provide spectacular advances in human health care (eg, via personalised medicine) provided that appropriate high-throughput biological reading devices can be developed. In developing such devices, this project also aims to substantially catalyse the Australian Nanotechnology/Biotechnology industry.Read moreRead less
Nanotechnology in nature: the evolutionary significance of iridescent ultraviolet colouration in butterflies. Nanostructural colour is a novel and interesting biological phenomenon that has potential application in textile and paint industries. This research has the potential to uncover knowledge relevant to future genetic manipulation and/or artificial synthesis of this trait for industry. Fundamental benefits will include a contribution to our understanding of evolution and biological diversit ....Nanotechnology in nature: the evolutionary significance of iridescent ultraviolet colouration in butterflies. Nanostructural colour is a novel and interesting biological phenomenon that has potential application in textile and paint industries. This research has the potential to uncover knowledge relevant to future genetic manipulation and/or artificial synthesis of this trait for industry. Fundamental benefits will include a contribution to our understanding of evolution and biological diversity, enhancement of Australia's research profile, and the cultivation of new scientific expertise. This proposal also promises to benefit the Australian scientific community through the establishment of collaborative links with universities in the USA and UK, and to increase mainstream awareness of Australia's stunning natural resources.Read moreRead less
Intravital super-resolution imaging via Stimulated Emission Depletion microscopy (STED)-microendoscopy. We will develop a new technology to enable the imaging of sub-cellular structures within a biological specimen, with super-resolution. This intravital super-resolution imaging technology will build off world leading techniques to image objects with super-resolution and to perform this within a specimen, with minimal invasion. The broad ramifications of this technology apply to biology, medical ....Intravital super-resolution imaging via Stimulated Emission Depletion microscopy (STED)-microendoscopy. We will develop a new technology to enable the imaging of sub-cellular structures within a biological specimen, with super-resolution. This intravital super-resolution imaging technology will build off world leading techniques to image objects with super-resolution and to perform this within a specimen, with minimal invasion. The broad ramifications of this technology apply to biology, medical science, imaging and sensing. Important applications include the early detection of debilitating diseases and the advancement of understanding of cellular biology. This research will raise Australia's profile as a world leader in science and technology, building on our emerging presence in the biophysical sciences.Read moreRead less
Quantum coherence of electronic transport in layered magnetoresistive materials. The continued rapid expansion of information technology requires new materials and devices for information storage. State of the art computer memories are based on new materials which consist of layers of complex arrays of atoms. These materials have metallic properties quite unlike those of simple metals such as copper and steel. This research will lead to a greater understanding of and ability to design better ma ....Quantum coherence of electronic transport in layered magnetoresistive materials. The continued rapid expansion of information technology requires new materials and devices for information storage. State of the art computer memories are based on new materials which consist of layers of complex arrays of atoms. These materials have metallic properties quite unlike those of simple metals such as copper and steel. This research will lead to a greater understanding of and ability to design better materials. Australia's capacity for research and development in this scientifically challenging and technologically important field will be enhanced by this project. Read moreRead less
Light scattering in complex mesoscale systems. The aim of the proposed project is to develop tested theoretic models of, and test applications of, the scattering of light in complex mesoscale systems. This will include: light scattering by biological cells, including optical cancer detection, remote sensing of cell populations, flow cytometry and optical tweezers; optically driven micromachines, and nano- and micro-structured materials. These applications are of considerable technological, socia ....Light scattering in complex mesoscale systems. The aim of the proposed project is to develop tested theoretic models of, and test applications of, the scattering of light in complex mesoscale systems. This will include: light scattering by biological cells, including optical cancer detection, remote sensing of cell populations, flow cytometry and optical tweezers; optically driven micromachines, and nano- and micro-structured materials. These applications are of considerable technological, social, and economic importance, and significant advances are possible. Methods developed during this project will allow accurate theoretical calculations to be performed where only inadequate approximations have previously been possible.Read moreRead less
Multiplexed Molecular Reading of Protein Associations via Nanoscaled Devices. Current developments in Nanoscience and Nanotechnology hold many promises in terms of revolutionising our industrial base, transforming biology, medical science and practice. This project strives to achieve some of these goals by, for the first time, building and testing nano-scaled devices with the capability to rapidly ?read? information about complex protein associations. With the recent completion of the Human Ge ....Multiplexed Molecular Reading of Protein Associations via Nanoscaled Devices. Current developments in Nanoscience and Nanotechnology hold many promises in terms of revolutionising our industrial base, transforming biology, medical science and practice. This project strives to achieve some of these goals by, for the first time, building and testing nano-scaled devices with the capability to rapidly ?read? information about complex protein associations. With the recent completion of the Human Genome project, major opportunities exist to provide spectacular advances in human health care (eg, via novel diagnostics) provided that appropriate high-throughput biological reading devices can be developed. In developing such devices, this project also aims to catalyse the Australian Nanotechnology/Biotechnology industry.Read moreRead less