Regulation of the EphA3 receptor tyrosine kinase in vertebrate development. The Eph/ephrin system has a critical role in normal embryonic development. Amongst vertebrates, the EphA3 gene is one of the most highly conserved genes in this system with critical roles in development of the visual system and in other developmental processes. Understanding how this gene is regulated will help us to understand the critical role of EphA3 in the basic biology of humans and other animals. This knowledge ma ....Regulation of the EphA3 receptor tyrosine kinase in vertebrate development. The Eph/ephrin system has a critical role in normal embryonic development. Amongst vertebrates, the EphA3 gene is one of the most highly conserved genes in this system with critical roles in development of the visual system and in other developmental processes. Understanding how this gene is regulated will help us to understand the critical role of EphA3 in the basic biology of humans and other animals. This knowledge may also shed light on the basis of congenital abnormalities and other pathological processes and possibly help us to understand how to prevent or treat these conditions.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0775587
Funder
Australian Research Council
Funding Amount
$532,000.00
Summary
Correlating Genomics and Proteomics for Systems Biology: integrating the '-omics'. Acquisition of the infrastructure requested will maintain and extend the expertise developed by researchers in NSW and will allow retention and attraction of leading researchers who can contribute to understanding how genes and proteins interact in the development of the organism - the central focus of systems biology. The enhancement of the facility will allow a better understanding of biomolecular interactions ....Correlating Genomics and Proteomics for Systems Biology: integrating the '-omics'. Acquisition of the infrastructure requested will maintain and extend the expertise developed by researchers in NSW and will allow retention and attraction of leading researchers who can contribute to understanding how genes and proteins interact in the development of the organism - the central focus of systems biology. The enhancement of the facility will allow a better understanding of biomolecular interactions in health and disease, providing both community and national benefits. The focus of this LIEF application is to provide infrastructure platforms for the study of the systems biology of organisms and additional capacity by the facility for the expected increased demand for this technology in this new area. Read moreRead less
A Genomic analysis of macrophage differentiation: Epigenetic factors that determine transcriptional choices in a lineage dependant manner. Our genetic information is fundamental to who we are, how we develop, & how we age. This project will build the research capacity of Australia's genome sciences, providing an analytical framework to describe & study the many products expressed from any single gene and to assess the function of genetic variation & test genome regulatory events. An immediate ou ....A Genomic analysis of macrophage differentiation: Epigenetic factors that determine transcriptional choices in a lineage dependant manner. Our genetic information is fundamental to who we are, how we develop, & how we age. This project will build the research capacity of Australia's genome sciences, providing an analytical framework to describe & study the many products expressed from any single gene and to assess the function of genetic variation & test genome regulatory events. An immediate outcome is a better understanding of the regulation of our immune system. This approach will fuel the discovery of new signalling molecules & their effects on a population of cells, & likewise provides a novel approach to study the dysregulation of cell signalling pathways.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0347245
Funder
Australian Research Council
Funding Amount
$630,000.00
Summary
Functional Genomics Analysis - linking a multicentred facility. The aim of this project is to enhance and network the functions and activities of the Clive and Vera Ramaciotti Centre for Gene Function Analysis (CGRCGFA), a joint venture that services five major universities in the Sydney-Newcastle area. This application is for equipment that will improve the speed of DNA analyses, and for a laboratory information management system that will standardise the handling of data and sample information ....Functional Genomics Analysis - linking a multicentred facility. The aim of this project is to enhance and network the functions and activities of the Clive and Vera Ramaciotti Centre for Gene Function Analysis (CGRCGFA), a joint venture that services five major universities in the Sydney-Newcastle area. This application is for equipment that will improve the speed of DNA analyses, and for a laboratory information management system that will standardise the handling of data and sample information at all nodes of the CVRCGFA.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0232455
Funder
Australian Research Council
Funding Amount
$545,000.00
Summary
The Molecular Analysis of Variation and Gene Function. The aim of this project is to establish the nodes of the Clive and Vera Ramaciotti Centre for Gene Function Analysis (CVRCGFA) which is a joint venture that serves the five major universities and three Institutes in the Sydney-Newcastle region. The primary focus of this application is to create new facilities at the hubs of CVRCFGA that are integral to the analysis of molecular variation in a range of organisms. The study of molecular vari ....The Molecular Analysis of Variation and Gene Function. The aim of this project is to establish the nodes of the Clive and Vera Ramaciotti Centre for Gene Function Analysis (CVRCGFA) which is a joint venture that serves the five major universities and three Institutes in the Sydney-Newcastle region. The primary focus of this application is to create new facilities at the hubs of CVRCFGA that are integral to the analysis of molecular variation in a range of organisms. The study of molecular variation will enable researchers to understand better how organisms interact with each other, how they respond to environmental stress and aid in the identification of complez traits.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453724
Funder
Australian Research Council
Funding Amount
$532,824.00
Summary
Functional Genomics - Linking Genomics and Proteomics. This application is to enhance the capacity of the Ramaciotti Centre for Gene Function Analaysis (RCFGA) and its Nodes to support research in Functional Genomics by expanding its ability to link genomic analysis (including transcriptomics) with proteomic and cell biological analysis. It will provide access to novel methods for analysing interactions of proteins with other molecules by enabling the manufacture of protein arrays as well as DN ....Functional Genomics - Linking Genomics and Proteomics. This application is to enhance the capacity of the Ramaciotti Centre for Gene Function Analaysis (RCFGA) and its Nodes to support research in Functional Genomics by expanding its ability to link genomic analysis (including transcriptomics) with proteomic and cell biological analysis. It will provide access to novel methods for analysing interactions of proteins with other molecules by enabling the manufacture of protein arrays as well as DNA microarrays and to analyse the data obtained from them. This is a vital component to maintain gene function analysis at the cutting edge of current technology.Read moreRead less
Genetic variation of transcriptional control. Genetic variation is a key cause phenotype differences in humans, animals, and plants and so of great economic importance. Despite its proven importance to human diseases, ?quantitative? variation in the amount of gene expression rather than ?qualitative? protein sequence changes, has not been systematically studied. We have developed a powerful method to identify genetic causes of quantitative variation using crosses of inbred mice in conjunction wi ....Genetic variation of transcriptional control. Genetic variation is a key cause phenotype differences in humans, animals, and plants and so of great economic importance. Despite its proven importance to human diseases, ?quantitative? variation in the amount of gene expression rather than ?qualitative? protein sequence changes, has not been systematically studied. We have developed a powerful method to identify genetic causes of quantitative variation using crosses of inbred mice in conjunction with microarray techniques to analyse expression of thousands of genes simultaneously. These studies will be extended to humans and be significant to wide areas of biological and commercial activity.Read moreRead less
Co-ordinated Action of ATM and DNA-PK in DNA damage recognition. The aim of this project is to investigate the mechanism of repair of double straind breaks in DNA sustained after radiation damage. Specifically we will focus on two proteins ATM (mutated in the genetic disorder ataxia-telangiectasia) and DNA-PK mutated in scid mice. There two proteins recognize double straind breaks in DNA and signal this damage to the DNA repair machinery of the cell and to cell cycle checkpoints. The emphasis ....Co-ordinated Action of ATM and DNA-PK in DNA damage recognition. The aim of this project is to investigate the mechanism of repair of double straind breaks in DNA sustained after radiation damage. Specifically we will focus on two proteins ATM (mutated in the genetic disorder ataxia-telangiectasia) and DNA-PK mutated in scid mice. There two proteins recognize double straind breaks in DNA and signal this damage to the DNA repair machinery of the cell and to cell cycle checkpoints. The emphasis here will be in the relationship between the two proteins in co-ordinating the repair of breaks in DNA. This information will be important in understanding mechanisms for maintaining the integrity of the genome.Read moreRead less
Mouse models for the identification of factors involved in muscle adaptation. The ability of muscle to adapt to meet functional demands is essential for mobility in normal daily life, in ageing well, in individuals with muscle diseases and nerve damage and in athletes. The ability of muscle to change its cellular composition is desirable for the livestock industry. Knowledge of how genes in muscle cells are regulated to adapt to demands has significant implications for public health and economic ....Mouse models for the identification of factors involved in muscle adaptation. The ability of muscle to adapt to meet functional demands is essential for mobility in normal daily life, in ageing well, in individuals with muscle diseases and nerve damage and in athletes. The ability of muscle to change its cellular composition is desirable for the livestock industry. Knowledge of how genes in muscle cells are regulated to adapt to demands has significant implications for public health and economic benefits. We have devised model systems that will allow us to identify the regulators of these genes in order to develop therapies to combat these changes in ageing and damaged muscle, to improve the quality of meat and optimise sport performance.Read moreRead less
Investigating the role of gene loops in regulating gene expression. The ability to identify functional variants in regulatory elements will have implications for researchers in multiple fields of biology, from molecular medicine to agriculture. Transfer of expertise and application of the knowledge generated by our research to such fields stands to improve diagnosis of disease predisposition and to improve quality of animal and plant products. These outcomes will benefit all Australians. This kn ....Investigating the role of gene loops in regulating gene expression. The ability to identify functional variants in regulatory elements will have implications for researchers in multiple fields of biology, from molecular medicine to agriculture. Transfer of expertise and application of the knowledge generated by our research to such fields stands to improve diagnosis of disease predisposition and to improve quality of animal and plant products. These outcomes will benefit all Australians. This knowledge will also improve the education of Australian University students as it contributes to the development of advanced curricula and access to more powerful research methods. In addition, the project will foster important collaborations between Australian researchers and those overseas.Read moreRead less