Development of an ultrasensitive assay for human prion proteins. The aim of this work is to enable the detection of prion proteins in human blood and other tissues. The assay system to be developed will detect much lower levels of these disease-causing proteins than is possible at present; it will be more rapid and will measure prion protein levels more accurately than existing assays. The outcome of the work is expected to facilitate the production and certification of prion-free blood and bl ....Development of an ultrasensitive assay for human prion proteins. The aim of this work is to enable the detection of prion proteins in human blood and other tissues. The assay system to be developed will detect much lower levels of these disease-causing proteins than is possible at present; it will be more rapid and will measure prion protein levels more accurately than existing assays. The outcome of the work is expected to facilitate the production and certification of prion-free blood and blood products. Prions cause Bovine Spongiform Encaphalopathy and Creutzfeld Jacob Disease and attempts to control of these diseases would be greatly abetted by an optimal test for the disease-causing agent.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453920
Funder
Australian Research Council
Funding Amount
$108,680.00
Summary
Molecular diagnostics based on real-time polymerase chain reactions for emerging tropical infectious diseases aimed at protecting Australia from invasive diseases. The project aims to use the technique of real-time polymerase chain reaction to rapidly detect and quantify the organisms associated with emerging and re-emerging infectious diseases of man and animals. It will also be used to determine related gene expression.
The equipment will be used to support a wide range of projects that req ....Molecular diagnostics based on real-time polymerase chain reactions for emerging tropical infectious diseases aimed at protecting Australia from invasive diseases. The project aims to use the technique of real-time polymerase chain reaction to rapidly detect and quantify the organisms associated with emerging and re-emerging infectious diseases of man and animals. It will also be used to determine related gene expression.
The equipment will be used to support a wide range of projects that require the detection of specific RNA or DNA and it will allow the rapid, cost effective and efficient processing of either RNA or DNA from large numbers of samples. Minor variations in organisms will be detected using this equipment.
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Control of Protein Attachment and its Optical Detection. Protein array technologies have applications in the rapid diagnosis of disease. Biosensors can detect traces of biohazards. Before widespread implementation of these technologies can occur however, a rapid, sensitive and convenient readout method for the control and readout of attachment of proteins to antibodies is needed. I will use electric fields, combined with array imaging at surface plasmon resonance to achieve this aim. This protei ....Control of Protein Attachment and its Optical Detection. Protein array technologies have applications in the rapid diagnosis of disease. Biosensors can detect traces of biohazards. Before widespread implementation of these technologies can occur however, a rapid, sensitive and convenient readout method for the control and readout of attachment of proteins to antibodies is needed. I will use electric fields, combined with array imaging at surface plasmon resonance to achieve this aim. This protein diagnostic array technology will enable accurate and rapid diagnosis of disease, generating savings on health costs and improving public health. Manufacture in Australia will bring further economic benefits.Read moreRead less