Developmental regulation of plant mitochondrial genome structure and copy number. Recombination is a major driving force behind mitochondrial DNA evolution and is responsible for the occurrence of cytoplasmic male sterile plants that are used by plant breeders to obtain high yield hybrids. A better understanding of the mechanisms that underlie mitochondrial and chloroplast genome maintenance and segregation will be a major fundamental scientific advance that will permit an integrated picture of ....Developmental regulation of plant mitochondrial genome structure and copy number. Recombination is a major driving force behind mitochondrial DNA evolution and is responsible for the occurrence of cytoplasmic male sterile plants that are used by plant breeders to obtain high yield hybrids. A better understanding of the mechanisms that underlie mitochondrial and chloroplast genome maintenance and segregation will be a major fundamental scientific advance that will permit an integrated picture of the interactions between the three plant genomes (nuclear, mitochondrial and chloroplastic). It is also a pre-requisite for the future manipulation of the cytoplasmic genomes leading to new ways to develop varieties with modified cytoplasms.Read moreRead less
Conductance states of a brain glutamine transporter. Brain transporters are the target for many neuroactive drugs that are used to treat anxiety, depression and other psychotic disorders. Transport processes are also targeted to deliver neurotransmitter precursors to the brain to treat disorders such as Parkinson's disease. In this project we will study a transport process crucial for the function of neurons that release glutamate and GABA (gamma-aminobutyric acid) as neurotransmitters. The stud ....Conductance states of a brain glutamine transporter. Brain transporters are the target for many neuroactive drugs that are used to treat anxiety, depression and other psychotic disorders. Transport processes are also targeted to deliver neurotransmitter precursors to the brain to treat disorders such as Parkinson's disease. In this project we will study a transport process crucial for the function of neurons that release glutamate and GABA (gamma-aminobutyric acid) as neurotransmitters. The study of this transport process will be important for understanding disorders like epilepsy and other disorders affecting neuronal excitability.Read moreRead less
The role of turgor in hyphal extension of the Ascomycete Neurospora crassa. Cellular expansion is an absolute necessity during the growth and development of plants and fungi. This process relies heavily upon the accumulation of inorganic ions. Osmotically driven water influx then creates the hydrostatic pressure that underlies the increase in cell volume. Cellular expansion is normally asymmetric and localised in one small region, such as hyphal tip. How does the cell maintain the turgor that dr ....The role of turgor in hyphal extension of the Ascomycete Neurospora crassa. Cellular expansion is an absolute necessity during the growth and development of plants and fungi. This process relies heavily upon the accumulation of inorganic ions. Osmotically driven water influx then creates the hydrostatic pressure that underlies the increase in cell volume. Cellular expansion is normally asymmetric and localised in one small region, such as hyphal tip. How does the cell maintain the turgor that drives expansion? How is expansion controlled spatially? These questions will be addressed in this project by comprehensive study of ion transport processes in a model organism, Neurospora crassa, using osmotic sensitive and transport mutants.Read moreRead less
Development of ultrastable high flux hydrophobic pervaporation membranes for organic separations. Perfluorinated glassy polymers (Teflons AF) as membrane materials are endowed with unique fluxes and excellent stability when in contact with various liquid organic mixtures. However, they have never been explored as membrane materials for pervaporation. The aims of this project are therefore to evaluate the applicability of composite membranes based on amorphous Teflons AF to the separation of orga ....Development of ultrastable high flux hydrophobic pervaporation membranes for organic separations. Perfluorinated glassy polymers (Teflons AF) as membrane materials are endowed with unique fluxes and excellent stability when in contact with various liquid organic mixtures. However, they have never been explored as membrane materials for pervaporation. The aims of this project are therefore to evaluate the applicability of composite membranes based on amorphous Teflons AF to the separation of organic mixtures and the removal of organic pollutants from water. The outcomes of this project will be the development of novel environmentally friendly processes in the petrochemical and biotechnology industries as well as ecologically efficient methods of waste water treatment.Read moreRead less
A high-through-put method for unlocking the mitochondrial genomes of significant pathogens. The national/community benefits of this research are: (1) to develop a long-term, high quality scientific and technological program contributing to national objectives, including the maintenance of a strong capability in basic research, the development of new scientific concepts and the enhancement of international collaborative links; (2) to strengthen the links between basic and applied research; (3) to ....A high-through-put method for unlocking the mitochondrial genomes of significant pathogens. The national/community benefits of this research are: (1) to develop a long-term, high quality scientific and technological program contributing to national objectives, including the maintenance of a strong capability in basic research, the development of new scientific concepts and the enhancement of international collaborative links; (2) to strengthen the links between basic and applied research; (3) to develop excellence in research by promoting collaborative research, resulting in a more efficient use of resources in a national and international context; (4) to enhance the skills-base in biology and biotechnology; and (5) to substantially increase global visibility through quality research, leading to an increased investment in Australian science.Read moreRead less
Nanostructures of Surfactants at Solid-Liquid and Gas-Liquid Interfaces and Interfacial Properties. The proposed research aims to investigate nanostructures of surface-active agents at interfaces and related interfacial properties. Specifically, the proposal focuses on quantitative models for the self-assembly of surfactants at solid-liquid and gas-liquid interfaces, experimental validation of the models and interfacial properties produced by the surfactants for industrial applications. The stud ....Nanostructures of Surfactants at Solid-Liquid and Gas-Liquid Interfaces and Interfacial Properties. The proposed research aims to investigate nanostructures of surface-active agents at interfaces and related interfacial properties. Specifically, the proposal focuses on quantitative models for the self-assembly of surfactants at solid-liquid and gas-liquid interfaces, experimental validation of the models and interfacial properties produced by the surfactants for industrial applications. The study will increase our understanding of surfactant-interface interaction to allow us to have greater control over the interfacial properties and improve the interface-based industrial processes. The research is relevant to the minerals and coal processing industry worth hundreds of billions dollars annually to Australia and USA.Read moreRead less
A rational approach to a high-resolution structure of the multidrug transporter EmrE. Membrane proteins form only 0.3% of the available protein structures in the protein data bank (PDB), yet 30% of the proteins in the human genome and 50% of human drug targets are membrane proteins. Multidrug transporters are membrane proteins responsible for antibiotic resistance in humans. A high-resolution structure of a multidrug resistance protein, together with comprehensive biochemical characterization, w ....A rational approach to a high-resolution structure of the multidrug transporter EmrE. Membrane proteins form only 0.3% of the available protein structures in the protein data bank (PDB), yet 30% of the proteins in the human genome and 50% of human drug targets are membrane proteins. Multidrug transporters are membrane proteins responsible for antibiotic resistance in humans. A high-resolution structure of a multidrug resistance protein, together with comprehensive biochemical characterization, would enable a detailed understanding of how these protein functions. Potentially it could also aid in the development of specific inhibitors that would prevent EmrE (and perhaps other similar proteins) from carry out its harmful mission. Read moreRead less
University of Queensland/Arizona State University partnership to design industrially suitable zeolite membranes for desalination. For desalination, the highest costs are organic-based membrane replacement (lasting ~1 year) and energy requirement. Functionalised zeolitic membranes are low-cost, high performing, chemically tolerant and thermally stable. New zeolite membranes in principle could perform the separation outlasting their organic counterparts, while at the same time offering major energ ....University of Queensland/Arizona State University partnership to design industrially suitable zeolite membranes for desalination. For desalination, the highest costs are organic-based membrane replacement (lasting ~1 year) and energy requirement. Functionalised zeolitic membranes are low-cost, high performing, chemically tolerant and thermally stable. New zeolite membranes in principle could perform the separation outlasting their organic counterparts, while at the same time offering major energy reductions from higher fluxes. Current zeolite membrane research for desalination however is lacking. The proposed team offers experience in bringing highly significant lab scale technologies to industrial scales. The outcomes will address mutual priorities between Australia and USA for reliable low cost supply of fresh water.Read moreRead less
Frictional and viscous effects during transport in nanopores. Gas mixtures exposed to materials containing molecular sized pores (nanopores) are adsorbed, forming states of matter not existing in the bulk. Differential forces acting on the components of mixtures promote industrially and environmentally important separations. However, transport of confined fluids has been poorly understood. Recently we have made progress using computer simulation, and have proposed a highly successful theory. ....Frictional and viscous effects during transport in nanopores. Gas mixtures exposed to materials containing molecular sized pores (nanopores) are adsorbed, forming states of matter not existing in the bulk. Differential forces acting on the components of mixtures promote industrially and environmentally important separations. However, transport of confined fluids has been poorly understood. Recently we have made progress using computer simulation, and have proposed a highly successful theory. These investigations have revealed the crucial role of scattering from the surface atoms of the confining solid. Theoretical investigation of single spherical species has been initiated, and extension to other species and to mixtures is now envisaged.Read moreRead less
In situ measurements of the electrostatic properties inside photosynthetic reaction centres: correlation with the energy conversion function of the protein. The photochemical reaction centre is a key protein complex involved in energy conversion. It converts solar energy into chemical energy as a transmembrane charge separation. Coupling of electron and proton transfer is catalysed at the level of a ubiquinone cofactor. In order to understand how the redox properties of this cofactor are fine tu ....In situ measurements of the electrostatic properties inside photosynthetic reaction centres: correlation with the energy conversion function of the protein. The photochemical reaction centre is a key protein complex involved in energy conversion. It converts solar energy into chemical energy as a transmembrane charge separation. Coupling of electron and proton transfer is catalysed at the level of a ubiquinone cofactor. In order to understand how the redox properties of this cofactor are fine tuned by the protein environment, we plan to probe the ubiquinone site using a voltage-sensitive fluorescent dye. This exciting multidisciplinary project will contribute to the understanding of how protein matrices influence and govern the midpoint redox potential of their cofactors and the environments of theirRead moreRead less