A rational approach to a high-resolution structure of the multidrug transporter EmrE. Membrane proteins form only 0.3% of the available protein structures in the protein data bank (PDB), yet 30% of the proteins in the human genome and 50% of human drug targets are membrane proteins. Multidrug transporters are membrane proteins responsible for antibiotic resistance in humans. A high-resolution structure of a multidrug resistance protein, together with comprehensive biochemical characterization, w ....A rational approach to a high-resolution structure of the multidrug transporter EmrE. Membrane proteins form only 0.3% of the available protein structures in the protein data bank (PDB), yet 30% of the proteins in the human genome and 50% of human drug targets are membrane proteins. Multidrug transporters are membrane proteins responsible for antibiotic resistance in humans. A high-resolution structure of a multidrug resistance protein, together with comprehensive biochemical characterization, would enable a detailed understanding of how these protein functions. Potentially it could also aid in the development of specific inhibitors that would prevent EmrE (and perhaps other similar proteins) from carry out its harmful mission. Read moreRead less
Nanostructures of Surfactants at Solid-Liquid and Gas-Liquid Interfaces and Interfacial Properties. The proposed research aims to investigate nanostructures of surface-active agents at interfaces and related interfacial properties. Specifically, the proposal focuses on quantitative models for the self-assembly of surfactants at solid-liquid and gas-liquid interfaces, experimental validation of the models and interfacial properties produced by the surfactants for industrial applications. The stud ....Nanostructures of Surfactants at Solid-Liquid and Gas-Liquid Interfaces and Interfacial Properties. The proposed research aims to investigate nanostructures of surface-active agents at interfaces and related interfacial properties. Specifically, the proposal focuses on quantitative models for the self-assembly of surfactants at solid-liquid and gas-liquid interfaces, experimental validation of the models and interfacial properties produced by the surfactants for industrial applications. The study will increase our understanding of surfactant-interface interaction to allow us to have greater control over the interfacial properties and improve the interface-based industrial processes. The research is relevant to the minerals and coal processing industry worth hundreds of billions dollars annually to Australia and USA.Read moreRead less
Development of ultrastable high flux hydrophobic pervaporation membranes for organic separations. Perfluorinated glassy polymers (Teflons AF) as membrane materials are endowed with unique fluxes and excellent stability when in contact with various liquid organic mixtures. However, they have never been explored as membrane materials for pervaporation. The aims of this project are therefore to evaluate the applicability of composite membranes based on amorphous Teflons AF to the separation of orga ....Development of ultrastable high flux hydrophobic pervaporation membranes for organic separations. Perfluorinated glassy polymers (Teflons AF) as membrane materials are endowed with unique fluxes and excellent stability when in contact with various liquid organic mixtures. However, they have never been explored as membrane materials for pervaporation. The aims of this project are therefore to evaluate the applicability of composite membranes based on amorphous Teflons AF to the separation of organic mixtures and the removal of organic pollutants from water. The outcomes of this project will be the development of novel environmentally friendly processes in the petrochemical and biotechnology industries as well as ecologically efficient methods of waste water treatment.Read moreRead less
In situ measurements of the electrostatic properties inside photosynthetic reaction centres: correlation with the energy conversion function of the protein. The photochemical reaction centre is a key protein complex involved in energy conversion. It converts solar energy into chemical energy as a transmembrane charge separation. Coupling of electron and proton transfer is catalysed at the level of a ubiquinone cofactor. In order to understand how the redox properties of this cofactor are fine tu ....In situ measurements of the electrostatic properties inside photosynthetic reaction centres: correlation with the energy conversion function of the protein. The photochemical reaction centre is a key protein complex involved in energy conversion. It converts solar energy into chemical energy as a transmembrane charge separation. Coupling of electron and proton transfer is catalysed at the level of a ubiquinone cofactor. In order to understand how the redox properties of this cofactor are fine tuned by the protein environment, we plan to probe the ubiquinone site using a voltage-sensitive fluorescent dye. This exciting multidisciplinary project will contribute to the understanding of how protein matrices influence and govern the midpoint redox potential of their cofactors and the environments of theirRead moreRead less
The response of mitochondria to oxidative stress in plants. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen atta ....The response of mitochondria to oxidative stress in plants. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen attack, and in production of reactive oxygen molecules. This proposal seeks to investigate how mitochondria are involved in these processes, focusing on the role of terminal oxidases. Potential outcomes include crops better able to cope with environmental stress.Read moreRead less