Establishing Design Principles Of Polymers For Intracellular Delivery . Engineered polymers have played a central role in the field of bionanotechnology by enabling targeted nanoscale cell interactions. Progress in the field of intracellular delivery is currently affected by a major bottleneck due to the absence of effective polymers that is applicable across the range of bimolecular cargoes. In essence depending on the type of cargo: DNA, RNA or protien, the polymer needs programmability. The l ....Establishing Design Principles Of Polymers For Intracellular Delivery . Engineered polymers have played a central role in the field of bionanotechnology by enabling targeted nanoscale cell interactions. Progress in the field of intracellular delivery is currently affected by a major bottleneck due to the absence of effective polymers that is applicable across the range of bimolecular cargoes. In essence depending on the type of cargo: DNA, RNA or protien, the polymer needs programmability. The limited tunability of traditional polymers agents makes them unsuitable for this particular application. The multidisciplinary project addresses this significant problem by engineering novel sequences of defined polymer based nanoscale agents to achieve efficient delivery in cells.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE230101542
Funder
Australian Research Council
Funding Amount
$450,154.00
Summary
Impact of humoral immunity on nanoparticle–biological interactions. This project aims to improve the biological applications of nanomaterials by understanding their fundamental interactions with proteins and cells in relevant biological environments. This will create new knowledge on how humoral (antibody-mediated) immunity affects nanomaterials using cutting-edge immunoassays, bio–nano characterisation techniques, and bioinformatics. Expected outcomes of the project include an understanding of ....Impact of humoral immunity on nanoparticle–biological interactions. This project aims to improve the biological applications of nanomaterials by understanding their fundamental interactions with proteins and cells in relevant biological environments. This will create new knowledge on how humoral (antibody-mediated) immunity affects nanomaterials using cutting-edge immunoassays, bio–nano characterisation techniques, and bioinformatics. Expected outcomes of the project include an understanding of how specific antibodies modulate the protein coatings on nanomaterials, which will shed light on how immune cells interact with nanomaterials. This will lead to design principles for nanomaterial properties to improve their effectiveness in delivering drugs and gene therapies.Read moreRead less
Bespoke nanomaterials for understanding nano-bio interactions under flow. This project aims to develop innovative scalable synthesis techniques to produce polymeric nanomaterials with controlled properties and characterise interactions between nanomaterials and cells under flow conditions. This project expects to generate new knowledge in priority research areas of nanotechnology, polymer chemistry and immunology. The outcome of this project is an original scalable and environmentally friendly t ....Bespoke nanomaterials for understanding nano-bio interactions under flow. This project aims to develop innovative scalable synthesis techniques to produce polymeric nanomaterials with controlled properties and characterise interactions between nanomaterials and cells under flow conditions. This project expects to generate new knowledge in priority research areas of nanotechnology, polymer chemistry and immunology. The outcome of this project is an original scalable and environmentally friendly technology, new knowledge of cell-nanomaterial interactions and new design principles for nanoparticles with potential future applications in drug delivery, immunology and nanomedicine. This project should provide significant benefits to polymer, nanomaterial and pharmaceutical research and industry in Australia.Read moreRead less
Engineering nanoscale tools for cellular interrogation. The aim is to address fundamental hurdles to engineering seamless nanobiointerfaces between electroactive nanoscale tools and living cells. This is expected to allow efficient delivery of many bioactive cargo types into cells, intracellular sampling of cytosol contents, and probing of action potential, all at the cell—material interface. New, powerful, electroactive nanoscale tools that deliver precise spatio-temporal resolution and minimal ....Engineering nanoscale tools for cellular interrogation. The aim is to address fundamental hurdles to engineering seamless nanobiointerfaces between electroactive nanoscale tools and living cells. This is expected to allow efficient delivery of many bioactive cargo types into cells, intracellular sampling of cytosol contents, and probing of action potential, all at the cell—material interface. New, powerful, electroactive nanoscale tools that deliver precise spatio-temporal resolution and minimal invasiveness and perturbation are likely to transform ex-vivo cellular processes. The intended outcomes are crucial for maximising precision in engineering and implementing of ex-vivo cellular processes. Fundamental advances in knowledge may eventually be a platform for developing cell-based therapies.
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Impact of Biological Coatings on Nanoparticle–Immune Cell Interactions. Nanomaterials exposed to biological environments such as blood or lymph fluids rapidly adsorb a layer of biomolecules on their surface, forming a biomolecular corona, and profoundly altering their properties. This project aims to resolve the influence of biomolecular coronas on nanoparticle–immune cell interactions by combining particle engineering, immunology, proteomics and bioinformatic analysis. The project expected outc ....Impact of Biological Coatings on Nanoparticle–Immune Cell Interactions. Nanomaterials exposed to biological environments such as blood or lymph fluids rapidly adsorb a layer of biomolecules on their surface, forming a biomolecular corona, and profoundly altering their properties. This project aims to resolve the influence of biomolecular coronas on nanoparticle–immune cell interactions by combining particle engineering, immunology, proteomics and bioinformatic analysis. The project expected outcomes are to generate new knowledge in nanomaterial–immune cell behaviour and design principles for nanoparticles with prospective applications in the agricultural, veterinary and biomedical sectors.Read moreRead less
Precise, Cytosolic Dendrimer Delivery Systems. This project aims to use precisely targeted dendrimer technology to improve the delivery of poorly permeable molecules to their subcellular sites of action. Our cutting edge approach combines innovative phage screening techniques and advanced dendrimer synthesis. The outcomes of this proposal will be: 1) a targeting system that is manufacturable at scale and reasonable cost, 2) a dendrimer delivery system that is rapidly internalised into specifc ta ....Precise, Cytosolic Dendrimer Delivery Systems. This project aims to use precisely targeted dendrimer technology to improve the delivery of poorly permeable molecules to their subcellular sites of action. Our cutting edge approach combines innovative phage screening techniques and advanced dendrimer synthesis. The outcomes of this proposal will be: 1) a targeting system that is manufacturable at scale and reasonable cost, 2) a dendrimer delivery system that is rapidly internalised into specifc target cells and 3) bio-responsive dendrimers that promote delivery of their cargo into the cytosol. This work will strengthen a highly successful collaboration between the Australian biotech company Starpharma and Monash University, to design the next generation of nanomaterials delivery systems.Read moreRead less
Bioprogramming the behaviour of nanoparticles in live cells by nanoscopy . The project aims to develop safer materials that are sustainably sourced from sweet corn, and investigate using advanced imaging technologies, how these materials are processed in biological systems, including human and plant cells. This project expects to generate new knowledge in the optimal design of materials that can be used safely and effectively in biological applications in medicine and in agriculture. Expected ou ....Bioprogramming the behaviour of nanoparticles in live cells by nanoscopy . The project aims to develop safer materials that are sustainably sourced from sweet corn, and investigate using advanced imaging technologies, how these materials are processed in biological systems, including human and plant cells. This project expects to generate new knowledge in the optimal design of materials that can be used safely and effectively in biological applications in medicine and in agriculture. Expected outcomes of this multidisciplinary project include a library of highly biocompatible nanomaterials and expanded knowledge on imaging technologies and structure-function relationship of nanomaterials in biological cells. This should provide significant benefits, such as improved crop yields and safer transfection agents.Read moreRead less
Soft Plasmene Nanosheets for Stretchable Plasmonic Skins. Conventional plasmonic sensors and devices are rigid, planar, and not stretchable. This project aims to apply plasmene materials developed at Monash's Nanobionics lab to design highly stretchable plasmonic devices (artificial plasmonic skins). Systematic experimental and theoretical studies will be undertaken to understand how the plasmonic skins respond to strains and how they can be used for fabricating novel stretchable devices. Such s ....Soft Plasmene Nanosheets for Stretchable Plasmonic Skins. Conventional plasmonic sensors and devices are rigid, planar, and not stretchable. This project aims to apply plasmene materials developed at Monash's Nanobionics lab to design highly stretchable plasmonic devices (artificial plasmonic skins). Systematic experimental and theoretical studies will be undertaken to understand how the plasmonic skins respond to strains and how they can be used for fabricating novel stretchable devices. Such studies will generate important new knowledge of fabrication, characterisation, and modelling of stretchable plasmene, hence, contributing to further Australian standing in the field of nanotechnology and plasmonics. It may also incubate patentable technologies, bringing potential economic gains.Read moreRead less
Ultrastretchable, Highly Transparent, Wearable Gold Nanowire Generators. Next-generation wearable electronics should be thin, soft and even transparent, enabling applications impossible to achieve with traditional rigid electronics. Such future electronics will require disruptive soft skin-conformal energy devices to power. This project aims to develop a bi-modal gold nanowire percolation strategy to design ultrathin conductors that are electrically conductive, optically transparent and mechanic ....Ultrastretchable, Highly Transparent, Wearable Gold Nanowire Generators. Next-generation wearable electronics should be thin, soft and even transparent, enabling applications impossible to achieve with traditional rigid electronics. Such future electronics will require disruptive soft skin-conformal energy devices to power. This project aims to develop a bi-modal gold nanowire percolation strategy to design ultrathin conductors that are electrically conductive, optically transparent and mechanically stretchable. It expects to generate new knowledge in nanomaterials design and new technologies to fabricate skin-like invisible wearable generators. This should provide significant benefits in advancing Australian standing in the fields of nanotechnology and energy science, and bringing potential economic gains.Read moreRead less
Novel tools and nanotechnology to navigate intracellular trafficking. This project aims to investigate how material accesses different compartments inside cells, also known as trafficking. Using immunology, cell biology and nanotechnology, the project will manipulate intracellular trafficking to achieve specific cellular functions. Outcomes will also form the basis of intellectual property development for new products by Australian biotechnology companies. These products will improve veterinary ....Novel tools and nanotechnology to navigate intracellular trafficking. This project aims to investigate how material accesses different compartments inside cells, also known as trafficking. Using immunology, cell biology and nanotechnology, the project will manipulate intracellular trafficking to achieve specific cellular functions. Outcomes will also form the basis of intellectual property development for new products by Australian biotechnology companies. These products will improve veterinary and human health services, leading to increased productivity.Read moreRead less