Self-destructing CRISPR-constructs For Targeted Genome Editing In The Retina.
Funder
National Health and Medical Research Council
Funding Amount
$679,926.00
Summary
Despite the identification of specific mutations causing many inherited retinal dystrophies, all of these conditions are currently untreatable. We have established gene-editing techniques and have developed a novel mouse model, which will serve as a robust platform for testing different techniques of gene editing in the retina. No other group in the world is known to be using this platform for gene editing and our work will expedite the clinical translation of this technology.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0454133
Funder
Australian Research Council
Funding Amount
$101,000.00
Summary
Upgrade of Infrared Analytical Facility. This application seeks funding to upgrade the existing infrared analytical facility in the Central Science Laboratory (CSL) by installing a near infrared analyser.This instrument will provide analytical support to researchers in the fields of silviculture, agriculture and chemistry by providing a low cost, rapid and accurate analysis of the chemical and physical properties of large sample numbers.This instrument will dramatically reduce the project costs, ....Upgrade of Infrared Analytical Facility. This application seeks funding to upgrade the existing infrared analytical facility in the Central Science Laboratory (CSL) by installing a near infrared analyser.This instrument will provide analytical support to researchers in the fields of silviculture, agriculture and chemistry by providing a low cost, rapid and accurate analysis of the chemical and physical properties of large sample numbers.This instrument will dramatically reduce the project costs, compared with traditional wet analytical methods,and enable larger and statistically more significant data sets to be acquired.The expected outcomes will include a more rapid identification of the genes controlling plant characteristics and the development of targeted plant breeding programs of economic significance to Australia.The development of specific resins for gold extraction will be both economically and environmentally important.Read moreRead less
Quantitative genetics of Eucalyptus globulus. Eucalypt plantations in Australia have expanded rapidly over the last decade. With increasing market competition from overseas plantations for both pulp and solid wood products, Australia must maintain a competitive edge through efficiencies in production and product quality. Breeding and deployment of genetically superior planting stock is part of the solution. With global climate chance and requirements for re-afforestation in drier zones, there ....Quantitative genetics of Eucalyptus globulus. Eucalypt plantations in Australia have expanded rapidly over the last decade. With increasing market competition from overseas plantations for both pulp and solid wood products, Australia must maintain a competitive edge through efficiencies in production and product quality. Breeding and deployment of genetically superior planting stock is part of the solution. With global climate chance and requirements for re-afforestation in drier zones, there is an increasing requirement to genetically improve drought tolerance. This project will provide genetic information and strategies to back Eucalyptus globulus breeding and deployment programs for traditional as well as drier environments. Read moreRead less
Molecular tools for understanding, predicting and managing flowering and reproductive development in Brassica oleracea. This project integrates basic research with application to a significant horticultural industry, building on Australia's position as a leader in world research on molecular and genetic regulation of flowering. The project will strengthen Australia's research reputation in this field, provide new tools for cultivar screening in and management of B. oleracea seed crops. The proj ....Molecular tools for understanding, predicting and managing flowering and reproductive development in Brassica oleracea. This project integrates basic research with application to a significant horticultural industry, building on Australia's position as a leader in world research on molecular and genetic regulation of flowering. The project will strengthen Australia's research reputation in this field, provide new tools for cultivar screening in and management of B. oleracea seed crops. The project will deliver training for 3 PhD students at the interface between basic and applied research. A shortage of skilled scientists with ability to link understanding of plant development at the molecular/genetic level with improved crop management practices has been identified by the horticultural sector as a major challenge. Read moreRead less
Environmental and genetic control over blackwood (Acacia melanoxylon) timber colour, density, and recovery. The project will investigate the roles of environment and genetics in the expression of wood characteristics in blackwood. It will determine the potential heritability of valuable blackwood wood traits and provide a sound understanding of the basis of colour expression. Heartwood characteristics, particularly colour, density, ring width and knottiness determine the commercial value of blac ....Environmental and genetic control over blackwood (Acacia melanoxylon) timber colour, density, and recovery. The project will investigate the roles of environment and genetics in the expression of wood characteristics in blackwood. It will determine the potential heritability of valuable blackwood wood traits and provide a sound understanding of the basis of colour expression. Heartwood characteristics, particularly colour, density, ring width and knottiness determine the commercial value of blackwood. The limited research on these characteristics, all undertaken outside Australia, points to combined genetic and environmental control. By understanding the relative importance that environmental conditions and genetic material have in producing desirable wood traits, growers will be better able to realise the full commercial value of blackwood.Read moreRead less
Selection and deployment of browsing resistant Eucalyptus globulus and Eucalyptus nitens. Marsupial browsing is a significant problem for eucalypt plantation establishment in Australia. To date, browsing control has relied on the use of 1080 pesticide, but through political and social pressure this will cease in Tasmanian State Forests by December 2005. To develop new approaches to integrated pest management, we will explore the use of natural plant resistance to marsupial browsers. We will dete ....Selection and deployment of browsing resistant Eucalyptus globulus and Eucalyptus nitens. Marsupial browsing is a significant problem for eucalypt plantation establishment in Australia. To date, browsing control has relied on the use of 1080 pesticide, but through political and social pressure this will cease in Tasmanian State Forests by December 2005. To develop new approaches to integrated pest management, we will explore the use of natural plant resistance to marsupial browsers. We will determine the genetic and chemical basis of seedling resistance in Eucalyptus globulus and E. nitens, and investigate the stability of resistance though tree development and across different sites. Resistant germplasm will be identified and tested in operational plantings.Read moreRead less
The role of phytotoxins in ray blight disease of pyrethrum and significance of toxin tolerance to disease resistance. Ray blight disease, caused by Phoma ligulicola, is the greatest threat to the Australian pyrethrum industry causing $2.0M in annual losses. We aim to clarify the pathology of P.ligulicola in pyrethrum, focussing on the role of fungal toxins during infection. Toxin importance in disease will be determined through histology and induction of toxin- pathogen mutants. Toxins will be ....The role of phytotoxins in ray blight disease of pyrethrum and significance of toxin tolerance to disease resistance. Ray blight disease, caused by Phoma ligulicola, is the greatest threat to the Australian pyrethrum industry causing $2.0M in annual losses. We aim to clarify the pathology of P.ligulicola in pyrethrum, focussing on the role of fungal toxins during infection. Toxin importance in disease will be determined through histology and induction of toxin- pathogen mutants. Toxins will be characterised and used in tissue culture to select obtain toxin tolerant pyrethrum clones that will be evaluated for agronomic performance and disease resistance. Successful development of resistant clones will decrease production costs, minimise risk of fungicide resistance development and reduce environmental pollution.Read moreRead less
Breeding for improved Australian hop varieties by induced mutations. Hop (Humulus lupulus) plants are vital to the brewing industry. Their main commercial products are alpha-acids and oils, which contribute to the bitter taste and aroma of beer. Hops possess different alpha-acids, including humulone (desirable) and cohumulone (non-desirable), and different oils, including humulene (desirable). Recently, beta-acids have been identified as useful compounds for emerging markets. Induced mutagen ....Breeding for improved Australian hop varieties by induced mutations. Hop (Humulus lupulus) plants are vital to the brewing industry. Their main commercial products are alpha-acids and oils, which contribute to the bitter taste and aroma of beer. Hops possess different alpha-acids, including humulone (desirable) and cohumulone (non-desirable), and different oils, including humulene (desirable). Recently, beta-acids have been identified as useful compounds for emerging markets. Induced mutagenesis will be used to alter the genetic make-up of hops, to obtain varieties with: (i) higher humulone; (ii) lower cohumulone; (iii) higher humulene; and (iv) higher beta-acids. This research will greatly assist the Australian hop industry by short-cutting traditional breeding performed by crossing.Read moreRead less
Unravelling the relationship between growth and wood properties in temperate eucalypts. The most important biological traits affecting profit in eucalypt plantations grown for pulpwood are growth, wood density and pulp yield. Shrinkage and collapse are important for timber production. Little information is available on the size and direction of the correlations between these traits, yet they are important in determining selection response in breeding as well as environmental impacts on pulp and ....Unravelling the relationship between growth and wood properties in temperate eucalypts. The most important biological traits affecting profit in eucalypt plantations grown for pulpwood are growth, wood density and pulp yield. Shrinkage and collapse are important for timber production. Little information is available on the size and direction of the correlations between these traits, yet they are important in determining selection response in breeding as well as environmental impacts on pulp and sawlog yield. The collaborating growers and breeders have brought together a unique base of Eucalyptus globulus and E. nitens field trials that will allow an integrated quantitative and molecular genetic study of the interrelationships amongst these key profit traits.Read moreRead less
Investigation of the impact of malt haze active proteins to improve brewing efficiency and beer quality. Australia is a major world exporter of malting barley (~2 millon t/pa) and malt (600,000 t/pa), primarily to the rapidly expanding Asian economic development region. An additional 200,000 t/pa of malt is provided to the Australian domestic brewing industry. By improving the quality of Australian malting barley and optimising the cost of brewery colloidal stabilisation measures, we expect hi ....Investigation of the impact of malt haze active proteins to improve brewing efficiency and beer quality. Australia is a major world exporter of malting barley (~2 millon t/pa) and malt (600,000 t/pa), primarily to the rapidly expanding Asian economic development region. An additional 200,000 t/pa of malt is provided to the Australian domestic brewing industry. By improving the quality of Australian malting barley and optimising the cost of brewery colloidal stabilisation measures, we expect higher demand and prices for Australian malting barley and malt. This will help support the viability of rural communities and the value adding involved in the malting and brewing of their produce in Australia.Read moreRead less