Manipulation of apoptosis-related genes to generate novel disease resistances in banana. Bananas are one of Australia's most important fruit crops. However, the industry is continually threatened by numerous diseases including yellow and black Sigatoka, Fusarium wilt and bunchy top. Control of these diseases currently requires either extensive pesticide treatments or complex agronomic procedures, of which most are ineffective, expensive or environmentally damaging. The aim of this project is to ....Manipulation of apoptosis-related genes to generate novel disease resistances in banana. Bananas are one of Australia's most important fruit crops. However, the industry is continually threatened by numerous diseases including yellow and black Sigatoka, Fusarium wilt and bunchy top. Control of these diseases currently requires either extensive pesticide treatments or complex agronomic procedures, of which most are ineffective, expensive or environmentally damaging. The aim of this project is to utilise a novel strategy to generate transgenic bananas with resistance to these diseases. The benefit will be both economic and environmental as implementation should significantly reduce or eliminate pesticide use. Importantly, this strategy can potentially be extrapolated to many other crops.Read moreRead less
Gene identification and genetic marker analysis of herbicide resistance in Lolium rigidum. Herbicide resistance threatens the vibrant, export-focused Australian cropping industry and environmental sustainability. The Australian herbicide resistance problem is far greater than elsewhere in the world. Herbicide resistance must be avoided, managed and reversed to ensure profitable agriculture and an environmentally sustainable landscape. This research will contribute to the national wealth through ....Gene identification and genetic marker analysis of herbicide resistance in Lolium rigidum. Herbicide resistance threatens the vibrant, export-focused Australian cropping industry and environmental sustainability. The Australian herbicide resistance problem is far greater than elsewhere in the world. Herbicide resistance must be avoided, managed and reversed to ensure profitable agriculture and an environmentally sustainable landscape. This research will contribute to the national wealth through helping ensure the profitability of vital Australian export agricultural industries and the sustainability of the soil/land resource. This proposal will ensure that Australia leads international herbicide resistance research and will enable the capture of intellectual property and commercial opportunities. Read moreRead less
Generating broad and stable resistance to gemini- and nanoviruses. The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Toba ....Generating broad and stable resistance to gemini- and nanoviruses. The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Tobacco yellow dwarf mastrevirus. In this project, we aim to further develop this strategy into a broad and stable mechanism for generating resistance to all known geminiviruses and nanoviruses.Read moreRead less
Characterisation of interacting genes that condition avirulence and resistance between Phytophthora pathogens and soybean. Plant pathogens from the genus Phytophthora cause severe crop losses in Australia and worldwide. Resistance in host plants is frequently overcome by mutation of avirulence genes in the pathogen to create virulent new races. We will clone and characterise genes from Phytophthora sojae that control virulence and avirulence, and isolate soybean genes whose products interact w ....Characterisation of interacting genes that condition avirulence and resistance between Phytophthora pathogens and soybean. Plant pathogens from the genus Phytophthora cause severe crop losses in Australia and worldwide. Resistance in host plants is frequently overcome by mutation of avirulence genes in the pathogen to create virulent new races. We will clone and characterise genes from Phytophthora sojae that control virulence and avirulence, and isolate soybean genes whose products interact with the Phytophthora gene products. An understanding of the molecular mechanisms than enable current plant varieties to recognise Phytophthora pathogens leading to resistance or susceptibility will assist the development of novel strategies for durable disease resistance in soybean and other crop plants.Read moreRead less
A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants w ....A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants with nematodes. It is based on an Australian patent which has wide applicability in plant biotechnology. Nematode control will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Genomic Synteny in Legumes; Application to Crop Breeding. Synteny is defined as the degree of colinearity between the order of orthologous genes in chromosomes of related species. New data suggests that legumes share widespread synteny. Extensive genomic resources are being accumulated for the model legume, Medicago truncatula including the full genome sequence. We propose to use these resources to uncover patterns of synteny between M. truncatula and the cultivated legumes chickpeas, lupins, ....Genomic Synteny in Legumes; Application to Crop Breeding. Synteny is defined as the degree of colinearity between the order of orthologous genes in chromosomes of related species. New data suggests that legumes share widespread synteny. Extensive genomic resources are being accumulated for the model legume, Medicago truncatula including the full genome sequence. We propose to use these resources to uncover patterns of synteny between M. truncatula and the cultivated legumes chickpeas, lupins, faba-beans, lentils and lucerne. In addition to revealing patterns of chromosomal evolution in this large plant family, we will generate genetic maps and molecular markers for use in practical plant breeding of these important crops.Read moreRead less
Putting smells into context: Using in vivo technologies to understand plant-insect odour signalling. How insects find host plants is central to understanding their basic ecology and management. We use a novel cross-disciplinary approach to target a key pest insect's responses to a host-plant, genetically modified to smell different. This insect pest, the cotton bollworm, costs the Australian economy millions in lost revenue every year. Reliance on insecticides to control insect pests is exacerba ....Putting smells into context: Using in vivo technologies to understand plant-insect odour signalling. How insects find host plants is central to understanding their basic ecology and management. We use a novel cross-disciplinary approach to target a key pest insect's responses to a host-plant, genetically modified to smell different. This insect pest, the cotton bollworm, costs the Australian economy millions in lost revenue every year. Reliance on insecticides to control insect pests is exacerbated by increasing insecticidal resistance and is a major economic, environmental and health concern. Our research will open the door to novel control strategies that use the natural odours of plants to reduce their attractiveness to insect pests.Read moreRead less
Molecular dissection of resistance to subterranean clover mottle virus using Medicago truncatula. Plant virus diseases cause economic losses in most crop plants. Grain and pasture legumes are an important component of Australian agricultural cropping sytems, and provide human food and animal feed: they also contribute to higher yields of crops like cereals when grown in rotation, by providing nitrogen and a disease break. In this project, the 'model' legume, Medicago truncatula ('Barrel Medic') ....Molecular dissection of resistance to subterranean clover mottle virus using Medicago truncatula. Plant virus diseases cause economic losses in most crop plants. Grain and pasture legumes are an important component of Australian agricultural cropping sytems, and provide human food and animal feed: they also contribute to higher yields of crops like cereals when grown in rotation, by providing nitrogen and a disease break. In this project, the 'model' legume, Medicago truncatula ('Barrel Medic') will be used to identify a new virus resistance gene. The knowledge gained will be used to improve resistance to viruses in crop legumes, so reducing losses and aiding sustainability of production. This will support rural communities and the national economy.Read moreRead less
Isolation and characterization of cell signalling systems that activate or suppress apoptosis in pathogenic and symbiotic fungal:plant interactions. The key roles of programmed cell death (PCD) in plant disease are becoming apparent. This project will test the hypotheses that successful colonisation by arbuscular mycorrhizae in plant roots requires the plant to up-regulate inhibitors of PCD; that susceptibility in plants to necrotrophic fungal pathogens requires triggering of PCD; and that resis ....Isolation and characterization of cell signalling systems that activate or suppress apoptosis in pathogenic and symbiotic fungal:plant interactions. The key roles of programmed cell death (PCD) in plant disease are becoming apparent. This project will test the hypotheses that successful colonisation by arbuscular mycorrhizae in plant roots requires the plant to up-regulate inhibitors of PCD; that susceptibility in plants to necrotrophic fungal pathogens requires triggering of PCD; and that resistance is the result of activation of inhibitors of PCD. This international project will have important implications biologically and economically for control of plant disease and symbiosis, will train early career researchers in an area of international expertise and will strengthen collaboration between the US and Australian research groupRead moreRead less
Seed quality and disease resistance trait mapping in lentil (Lens culinaris ssp. culinaris). To protect and increase our world lentil market share, the mission of the Australian lentil breeders is to develop lentil varieties with superior seed qualities. Three highly desirable seed quality traits are; fatter and larger shape, better splitting efficiency and resistance to blemishing by ascochyta blight infection. To speed up the development of varieties with these traits, the genetic loci governi ....Seed quality and disease resistance trait mapping in lentil (Lens culinaris ssp. culinaris). To protect and increase our world lentil market share, the mission of the Australian lentil breeders is to develop lentil varieties with superior seed qualities. Three highly desirable seed quality traits are; fatter and larger shape, better splitting efficiency and resistance to blemishing by ascochyta blight infection. To speed up the development of varieties with these traits, the genetic loci governing them will be characterised and closely associated molecular markers identified. Markers will be validated for their use in marker-assisted breeding programs via back-crossing and field and glass house testing.Read moreRead less