Molecular, physiological and environmental regulation of toxic prussic acid levels (cyanogenesis) in forage sorghum. Forage sorghum is grown widely in dry, tropical areas of Australia. The leaves contain dhurrin, a natural defence product that liberates prussic acid (cyanide) when leaf tissue is disrupted (eg when chewed). The problem is that young plants or those experiencing drought are highly toxic, resulting in financial loss through reduced nutritive value, livestock loss and wasted feed. U ....Molecular, physiological and environmental regulation of toxic prussic acid levels (cyanogenesis) in forage sorghum. Forage sorghum is grown widely in dry, tropical areas of Australia. The leaves contain dhurrin, a natural defence product that liberates prussic acid (cyanide) when leaf tissue is disrupted (eg when chewed). The problem is that young plants or those experiencing drought are highly toxic, resulting in financial loss through reduced nutritive value, livestock loss and wasted feed. Using new, non-GM technology we will identify novel genetically altered sorghum lines with negligible prussic acid. Lines with enhanced levels could be used as soil biofumigants. Breeders can use this germplasm to develop varieties tailored for increasingly dry Australian conditions. The new varieties with controlled dhurrin content will be suitable for export.Read moreRead less
Integrated genetic regulation of photomorphogenesis in Pisum. This project will use a molecular genetic approach in garden pea to investigate the roles of photoreceptors that mediate developmental responses to light. It will define gene families encoding phytochrome, cryptochrome and phototropin photoreceptors, characterise photoreceptor gene expression, and identify mutants with impaired response to light. The mutants will be used in molecular, physiological and biochemical studies to examine h ....Integrated genetic regulation of photomorphogenesis in Pisum. This project will use a molecular genetic approach in garden pea to investigate the roles of photoreceptors that mediate developmental responses to light. It will define gene families encoding phytochrome, cryptochrome and phototropin photoreceptors, characterise photoreceptor gene expression, and identify mutants with impaired response to light. The mutants will be used in molecular, physiological and biochemical studies to examine how photoreceptors control and co-ordinate development throughout the plant via effects on plant hormone synthesis and response. Results from the project will be of practical importance in manipulating key aspects of plant growth to better suit particular environmental and agronomic objectives.Read moreRead less
IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the A ....IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the AMT family of ammonium transporters and associated signalling pathways which control the uptake and assimilation of ammonium in plants. This project will confirm the mechanisms involved in ammonium uptake from the soil and lead to the development of ammonium-nitrogen efficient crop plants.Read moreRead less
The role of plant hormones in arbuscular mycorrhizal symbiosis. The vast majority of plant species can form a beneficial symbiosis with specialised soil fungi, an association that can enhance the uptake of nutrients from the soil, improve tolerance to drought and disease and minimise soil erosion. An understanding of how plants establish and regulate this important symbiosis has the potential to contribute to the development of productive and sustainable farming systems by making efficient use o ....The role of plant hormones in arbuscular mycorrhizal symbiosis. The vast majority of plant species can form a beneficial symbiosis with specialised soil fungi, an association that can enhance the uptake of nutrients from the soil, improve tolerance to drought and disease and minimise soil erosion. An understanding of how plants establish and regulate this important symbiosis has the potential to contribute to the development of productive and sustainable farming systems by making efficient use of the limited water resources, reducing soil erosion, reducing reliance on pesticides and fertilisers and producing more nutritious fruits, vegetables and grains.Read moreRead less
Stomatal function in transgenic plants with altered guard cell metabolism. Guard cells on the surface of leaves control the rate of water loss and CO2 uptake by changing stomatal aperture in response to environmental signals such light, CO2, humidity and water status. Guard cells therefore play a major role in determining plant productivity and water use efficiency. This project aims to examine the contribution of guard cell energy and carbon metabolism in mediating stomatal responses to the env ....Stomatal function in transgenic plants with altered guard cell metabolism. Guard cells on the surface of leaves control the rate of water loss and CO2 uptake by changing stomatal aperture in response to environmental signals such light, CO2, humidity and water status. Guard cells therefore play a major role in determining plant productivity and water use efficiency. This project aims to examine the contribution of guard cell energy and carbon metabolism in mediating stomatal responses to the environment in intact plants through the generation and analysis of transgenic plants with altered guard cell function. This will aid in the development of strategies for direct manipulation of stomatal function.Read moreRead less
Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We ....Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We will generate antibody fragments that bind these MP and express them in tomato. We will analyse antibody expression in tomato and study their association with the phytoplasma membrane. Transgenic plants will be inoculated with phytoplasma and monitored for resistance. This work represents a major step towards engineering resistance to phytoplasmas.Read moreRead less
The Potential of the Fungicide Phosphite to Control the Autonomous Spread of Phytophthora cinnamomi in Natural and Rehabilitated Ecosystems. Phytophthora cinnamomi is recognised by the Federal Government as a key threatening process to Australia's biodiversity. This project will enhance the existing methodologies and protocols to improve the effectiveness and persistence of phosphite to reduce or contain the autonomous spread of this pathogen through susceptible and threatened plant communities. ....The Potential of the Fungicide Phosphite to Control the Autonomous Spread of Phytophthora cinnamomi in Natural and Rehabilitated Ecosystems. Phytophthora cinnamomi is recognised by the Federal Government as a key threatening process to Australia's biodiversity. This project will enhance the existing methodologies and protocols to improve the effectiveness and persistence of phosphite to reduce or contain the autonomous spread of this pathogen through susceptible and threatened plant communities. It will provide environmental, mining and land-care organisations with improved techniques to control P. cinnamomi in a range of plant communities and environments associated with mining and natural ecosystems.Read moreRead less
Are flavonoids metabolic regulators of plant development? This project will investigate the mechanisms of action of flavonoids, which are abundant and diverse plant products contained in all fruits and vegetables. We have very little knowledge on the range of activities this large class of natural compounds has in plants. This research will investigate the role of flavonoids in regulating plant development to identify flavonoids and their target proteins and genes that could alter plant develo ....Are flavonoids metabolic regulators of plant development? This project will investigate the mechanisms of action of flavonoids, which are abundant and diverse plant products contained in all fruits and vegetables. We have very little knowledge on the range of activities this large class of natural compounds has in plants. This research will investigate the role of flavonoids in regulating plant development to identify flavonoids and their target proteins and genes that could alter plant development in specific ways to create improved crops. This project will also strengthen Australia's expertise in proteomics, an important tool for the advancement of knowledge and application in biotechnology.Read moreRead less
Characterisation of interacting genes that condition avirulence and resistance between Phytophthora pathogens and soybean. Plant pathogens from the genus Phytophthora cause severe crop losses in Australia and worldwide. Resistance in host plants is frequently overcome by mutation of avirulence genes in the pathogen to create virulent new races. We will clone and characterise genes from Phytophthora sojae that control virulence and avirulence, and isolate soybean genes whose products interact w ....Characterisation of interacting genes that condition avirulence and resistance between Phytophthora pathogens and soybean. Plant pathogens from the genus Phytophthora cause severe crop losses in Australia and worldwide. Resistance in host plants is frequently overcome by mutation of avirulence genes in the pathogen to create virulent new races. We will clone and characterise genes from Phytophthora sojae that control virulence and avirulence, and isolate soybean genes whose products interact with the Phytophthora gene products. An understanding of the molecular mechanisms than enable current plant varieties to recognise Phytophthora pathogens leading to resistance or susceptibility will assist the development of novel strategies for durable disease resistance in soybean and other crop plants.Read moreRead less
Transcriptome profiling of Phytophthora pathogenicity genes: regulation of cell wall degrading enzyme synthesis during plant infection. This project will catalogue the repertoire of enzymes produced by plant pathogens to break down plant cell walls during initial penetration and later establishment of disease. This project will determine how production of these enzymes is regulated and how their function is optimised to achieve successful plant infection.