Does a novel class of small RNA molecules control self-incompatibility in solanaceous plants? Self-incompatibility is a simple and genetically defined cell recognition system that prevents inbreeding in many plant species. Flowers of self-incompatible plants can distinguish self pollen from foreign pollen, and allow only foreign pollen to fertilise their egg cells. This proposal will investigate the possibility that the part of the genetic self-incompatibility locus controlling recognition of ....Does a novel class of small RNA molecules control self-incompatibility in solanaceous plants? Self-incompatibility is a simple and genetically defined cell recognition system that prevents inbreeding in many plant species. Flowers of self-incompatible plants can distinguish self pollen from foreign pollen, and allow only foreign pollen to fertilise their egg cells. This proposal will investigate the possibility that the part of the genetic self-incompatibility locus controlling recognition of pollen is a novel type of gene that encodes a small RNA molecule but no protein. Knowledge gained by studying the self-incompatibility genes will help us to understand how plant cells recognise each other, and may allow us to manipulate seed (and hence crop) production.Read moreRead less
The Role of High-Frequency Antigenic Variation in The Pathogenesis of Mycoplasma infection. The main goal of the proposed project is to understand the molecular mechanisms of phase/antigenic variation and its effects on mycoplasma pathogenesis. In this context I will use the well-characterised Mycoplasma synoviae haemagglutinin, MSPA, to establish the role of its phase-variable expression in the type and extent of M. synoviae disease. Additionally, the relationship between MSPA phase variation a ....The Role of High-Frequency Antigenic Variation in The Pathogenesis of Mycoplasma infection. The main goal of the proposed project is to understand the molecular mechanisms of phase/antigenic variation and its effects on mycoplasma pathogenesis. In this context I will use the well-characterised Mycoplasma synoviae haemagglutinin, MSPA, to establish the role of its phase-variable expression in the type and extent of M. synoviae disease. Additionally, the relationship between MSPA phase variation and gene rearrangements in the MSPB-encoding gene will be elucidated. The results will contribute to our understanding of the pathogenesis of bacterial disease and of the evolution of pathogenic mechanisms in bacterial pathogens.Read moreRead less
An Analysis of the Cultural, Social and Symbolic Performance of Computer-Generated, Post-Euclidean, Architecture in Australia. Computer Aided Design software has recently provided architects with the ability to use post-Euclidean geometry for the creation of buildings. As the first of these buildings are only now being completed the social, cultural and symbolic performance of this new approach to design remains unknown.
The present research seeks to redress this situation by analysing computer ....An Analysis of the Cultural, Social and Symbolic Performance of Computer-Generated, Post-Euclidean, Architecture in Australia. Computer Aided Design software has recently provided architects with the ability to use post-Euclidean geometry for the creation of buildings. As the first of these buildings are only now being completed the social, cultural and symbolic performance of this new approach to design remains unknown.
The present research seeks to redress this situation by analysing computer-generated public buildings, completed in Australia after 1998. These buildings represent a substantial outlay of public funding and a significant social investment in Australian cities.
This research will result in a critical evaluation strategy for public and institutional bodies interested in procuring such buildings.Read moreRead less
Modelling greenhouse gas emissions associated with commercial building construction. A reliable model of greenhouse gas emissions for commercial building construction is required. Construction product manufacturing emissions are well known, but those associated with the construction process (representing up to 25% of the energy embodied in construction products) have not been fully elucidated. Commercial building construction requires more energy per square metre than residential building constr ....Modelling greenhouse gas emissions associated with commercial building construction. A reliable model of greenhouse gas emissions for commercial building construction is required. Construction product manufacturing emissions are well known, but those associated with the construction process (representing up to 25% of the energy embodied in construction products) have not been fully elucidated. Commercial building construction requires more energy per square metre than residential building construction, due mainly to lifting of heavy items. The results will be used to develop cost effective strategies for optimising greenhouse gas emissions associated with the total emissions from commercial building construction and operation. This will improve the environmental performance of the Australian construction industry.Read moreRead less
CesA (cellulose synthase) genes of Arabidopsis; all doing the same job or specialists cooperating to make the most abundant biopolymer. The biosphere makes more cellulose than any other polymer with fibre industries depending on its physical properties and atmospheric carbon dioxide levels depending on its stability as a carbon sink. Demonstrations that cellulose production needs CesA genes drove recent progress in elucidating the mechanism of synthesis. CesA proteins all look very similar but i ....CesA (cellulose synthase) genes of Arabidopsis; all doing the same job or specialists cooperating to make the most abundant biopolymer. The biosphere makes more cellulose than any other polymer with fibre industries depending on its physical properties and atmospheric carbon dioxide levels depending on its stability as a carbon sink. Demonstrations that cellulose production needs CesA genes drove recent progress in elucidating the mechanism of synthesis. CesA proteins all look very similar but if all do the same job, why do plants need so many and why do none seem redundant? We will make gene interchanges in transgenic plants, build chimeric genes and identify where each CesA protein operates. This will identify their individual and cooperative contributions to cellulose production.Read moreRead less
How protein and RNA cargo are sorted into exosomes. This project aims to understand how proteins and RNA are selected for packaging into exosomes and participate in the biological functions mediated by these vesicles. Exosomes are small membranous extracellular vesicles released by cells which contain protein and RNA cargo and are involved in intercellular communication. Determining how the exosome cargo is selected and related to its function in intercellular communication is expected to show h ....How protein and RNA cargo are sorted into exosomes. This project aims to understand how proteins and RNA are selected for packaging into exosomes and participate in the biological functions mediated by these vesicles. Exosomes are small membranous extracellular vesicles released by cells which contain protein and RNA cargo and are involved in intercellular communication. Determining how the exosome cargo is selected and related to its function in intercellular communication is expected to show how these vesicles maintain cellular homeostasis. The findings will expand knowledge in the area of microRNA biology, proteomics and develop expertise in bioinformatics.Read moreRead less
Designer DNA-binding factors. This project aims to use a natural transcription factor family to enhance the efficiency and functionality of designer DNA-binding factors. Research into the structure and function of zinc finger transcription factors, TAL effectors and CRISPR created designer DNA-binding factors. However, though research has improved the specificity of these factors’ genome-wide binding, their efficacy in regulating the expression of genes requires improvement. Using sequencing, th ....Designer DNA-binding factors. This project aims to use a natural transcription factor family to enhance the efficiency and functionality of designer DNA-binding factors. Research into the structure and function of zinc finger transcription factors, TAL effectors and CRISPR created designer DNA-binding factors. However, though research has improved the specificity of these factors’ genome-wide binding, their efficacy in regulating the expression of genes requires improvement. Using sequencing, the project intends to enhance the efficiency and function of these factors by designing modules to improve the stability of DNA binding and effectiveness in functionally regulating gene expression. The project outcomes could include knowledge enabling the use of genetically engineered DNA-binding proteins to artificially control gene expression, with significant scientific and economic implications.Read moreRead less
Spatial Knowledge and the Built Environment: The Design Implications of Making, Processing and Digitally Prototyping Architectural Models. The construction industry accounts for 6.7% of Australia's GDP. Beyond the more pragmatic economic benefits, however, lies the potential to influence the physical quality and appearance of our nation's building within an existing economic framework. The evaluation of differing modelling methods in design will have considerable implications for the way in whic ....Spatial Knowledge and the Built Environment: The Design Implications of Making, Processing and Digitally Prototyping Architectural Models. The construction industry accounts for 6.7% of Australia's GDP. Beyond the more pragmatic economic benefits, however, lies the potential to influence the physical quality and appearance of our nation's building within an existing economic framework. The evaluation of differing modelling methods in design will have considerable implications for the way in which designing is carried out. It will lead to new kinds of computer strategies that will guide the direction of development of programs used in design modelling by showing that current tools do not properly facilitate certain kinds of design inquiry available by more traditionally established means.Read moreRead less
Proteomic and Transcriptional Profiling of Cartilage. Gene expression and signalling pathways that regulate cartilage formation, and its orderly transition to bone, are poorly described. Our studies will, for the first time, combine two complementary cutting-edge approaches, protein identification by proteomic analysis, and mRNA profiling by microarray analysis, to define these pathways and develop a comprehensive catalogue of proteins and gene expression patterns during cartilage development a ....Proteomic and Transcriptional Profiling of Cartilage. Gene expression and signalling pathways that regulate cartilage formation, and its orderly transition to bone, are poorly described. Our studies will, for the first time, combine two complementary cutting-edge approaches, protein identification by proteomic analysis, and mRNA profiling by microarray analysis, to define these pathways and develop a comprehensive catalogue of proteins and gene expression patterns during cartilage development and bone formation. This information will provide insight into the regulation of cartilage differentiation, maturation and structure, and will provide a critical platform for the development of more sophisticated cartilage and bone biomaterials for improved tissue repair and regeneration.Read moreRead less
Decorum as an Idea in Architecture, from the Renaissance to the Present. Examines the theory and practice of decorum in architecture from the Renaissance to the present day. The principal aims are to set decorum within the history of ideas and to establish the relevance of the concept to modernism and contemporary debates on the nature of architecture. The project will be empirically innovative and significant to both historians and theorists. The expected outcome is a book-length study.