Unsaturation of vapour pressure inside leaves: fundamental, but unknown. This project aims to determine when and to what extent the air inside leaves becomes unsaturated with water vapour. All current interpretation and modelling of leaf gas exchange assumes saturation under all circumstances. Compelling evidence has been obtained that suggests this is not true under moderate air vapour pressure deficits. A novel technique will be employed to assess the water vapour concentration of the air insi ....Unsaturation of vapour pressure inside leaves: fundamental, but unknown. This project aims to determine when and to what extent the air inside leaves becomes unsaturated with water vapour. All current interpretation and modelling of leaf gas exchange assumes saturation under all circumstances. Compelling evidence has been obtained that suggests this is not true under moderate air vapour pressure deficits. A novel technique will be employed to assess the water vapour concentration of the air inside leaves based on stable isotope analysis of carbon dioxide and water vapour exchanged between leaves and air. The project is expected to provide fundamental knowledge about how stomata regulate photosynthesis and water use, with significant implications for modelling vegetation function and for improving the performance of crop plants.Read moreRead less
Feasting on protein? Strategies of organic nitrogen acquisition by plant roots. Crops require large amounts of nitrogen for growth. Application of nitrogen fertiliser enhances yield, but causes off-site nitrogen pollution, a main threat to ecosystem integrity. Most nitrogen in soil occurs as organic complexes that are broken down by soil organism into small compounds, which are taken up roots or lost from the soil. This project will generate fundamental knowledge of how an Australian species and ....Feasting on protein? Strategies of organic nitrogen acquisition by plant roots. Crops require large amounts of nitrogen for growth. Application of nitrogen fertiliser enhances yield, but causes off-site nitrogen pollution, a main threat to ecosystem integrity. Most nitrogen in soil occurs as organic complexes that are broken down by soil organism into small compounds, which are taken up roots or lost from the soil. This project will generate fundamental knowledge of how an Australian species and a crop species with unusual root specialisations access soil organic nitrogen, thus increasing the efficiency of nitrogen use and reducing nitrogen loss. The research employs cutting-edge techniques for sustainable resource use, improved efficiency of crops and farming systems, and preservation of Australia's biodiversity.Read moreRead less
Will stomatal responses to humidity and carbon dioxide constrain tropical forest productivity as atmospheric carbon dioxide rises? This project will investigate two physiological processes that will partly determine growth responses of tropical forest trees to rising atmospheric carbon dioxide. The project will produce equations summarising physiological responses that can be incorporated into process-based models of tropical forest productivity.
Reading the isotopic archive: carbon and oxygen stable isotope ratios as recorders of plant physiological processes. This project will investigate how plant physiological processes are reflected in stable isotope ratios of carbon and oxygen in plant tissues. Results will contribute towards a mechanistic understanding of the processes that cause isotopic modifications, thereby enabling an improved interpretation of naturally occurring stable isotope signals.
Beyond the gene: Linking herbivore behaviour to plant defense gene expression. This collaborative project investigates insect herbivore avoidance of plant defence mechanisms. Our project is novel because it integrates changes in the plant at a number of different levels and links them to insect foraging behaviour. Researchers assume that insects respond to plant defences by changing their foraging behaviour. This has not been tested directly. We use the genetically well characterised plant Arabi ....Beyond the gene: Linking herbivore behaviour to plant defense gene expression. This collaborative project investigates insect herbivore avoidance of plant defence mechanisms. Our project is novel because it integrates changes in the plant at a number of different levels and links them to insect foraging behaviour. Researchers assume that insects respond to plant defences by changing their foraging behaviour. This has not been tested directly. We use the genetically well characterised plant Arabidopsis and the world-wide pest Helicoverpa (heliothis) as a model system. Damage caused to crops by insect herbivores is a direct function of behaviour. Understanding this behaviour will lead to improved pest management and reduced economic losses.Read moreRead less
Regulation of ion homeostasis by two-component signalling elements. Dryland salinity is continuously claiming Australian lands and is a serious threat to our agricultural industry, native flora and fauna, and infrastructure. Attempting to feed an increasing population whilst agricultural land is disappearing, places an ever-increasing burden on our remaining land. While there is no simple solution to this problem, understanding how plants regulate cellular ion concentrations will help to generat ....Regulation of ion homeostasis by two-component signalling elements. Dryland salinity is continuously claiming Australian lands and is a serious threat to our agricultural industry, native flora and fauna, and infrastructure. Attempting to feed an increasing population whilst agricultural land is disappearing, places an ever-increasing burden on our remaining land. While there is no simple solution to this problem, understanding how plants regulate cellular ion concentrations will help to generate plants that are suitable for restoration of damaged lands or crop plants that are more tolerant to saline soils. Furthermore, knowledge acquired from the proposed project will also be useful for generating healthier crop plants with enhanced levels of ions essential for the human diet. Read moreRead less
Systemic control of nodule proliferation. We aim to clone and characterize the functions of the supernodulation (NTS-1) locus of soybean using positional cloning and functional genomics approaches. Supernodulation fascinatingly results from a mutant Nts-1 gene functioning in the shoot, although the phenotype is expressed as excessive nodule proliferation in the root. The cloned gene will be used to monitor expression changes after inoculation with Bradyrhizobium, treatment with nitrate, nod-fac ....Systemic control of nodule proliferation. We aim to clone and characterize the functions of the supernodulation (NTS-1) locus of soybean using positional cloning and functional genomics approaches. Supernodulation fascinatingly results from a mutant Nts-1 gene functioning in the shoot, although the phenotype is expressed as excessive nodule proliferation in the root. The cloned gene will be used to monitor expression changes after inoculation with Bradyrhizobium, treatment with nitrate, nod-factor, xylem exudates and phytohormones. We will use RT-PCR, in situ hybridisation and reporter gene expression in transgenic plants. Microarray analysis of soybean ESTs (4200 arrayed) will analyse concurrent gene expression changes in both root and shoot.Read moreRead less
Genetic and molecular analysis of long-distance gene silencing in Arabidopsis. Gene silencing is a surveillance mechanism in plants and animals to ensure that all genes are switched on or off at the right time. It is also a defence mechanism against viruses. Perturbation of gene silencing can be a cause of genetic diseases, and conversely, gene silencing has immense potential as a therapeutic tool for correcting genetic diseases and curing viral diseases. When silencing is triggered against a ge ....Genetic and molecular analysis of long-distance gene silencing in Arabidopsis. Gene silencing is a surveillance mechanism in plants and animals to ensure that all genes are switched on or off at the right time. It is also a defence mechanism against viruses. Perturbation of gene silencing can be a cause of genetic diseases, and conversely, gene silencing has immense potential as a therapeutic tool for correcting genetic diseases and curing viral diseases. When silencing is triggered against a gene or virus in plants, genetic signals are transmitted throughout the organism to systemically switch off the specific gene or virus. Expected long-term national/community benefits from understanding gene silencing are wide-ranging, from improved crops through to drugs and gene therapy.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE140100189
Funder
Australian Research Council
Funding Amount
$191,095.00
Summary
A shared mass spectrometer with compound-specific capabilities to support innovative research in biology, the environment and geology. A shared mass spectrometer with compound-specific capabilities to support innovative research in biology, the environment and geology: Stable isotope studies have huge and increasing relevance to environmental studies, many of which form the backbone of understanding Australia's terrestrial and marine systems. Compound-specific isotope analysis yields much more i ....A shared mass spectrometer with compound-specific capabilities to support innovative research in biology, the environment and geology. A shared mass spectrometer with compound-specific capabilities to support innovative research in biology, the environment and geology: Stable isotope studies have huge and increasing relevance to environmental studies, many of which form the backbone of understanding Australia's terrestrial and marine systems. Compound-specific isotope analysis yields much more information than is available through bulk methods. The problem has been that the separations were labour-intensive and employed complex wet chemistry. New methods reduce the work-load enough to make compound-specific studies possible. In the case of carbon isotopes, new liquid chromatographic technology removes the need for derivatisations which dilute the natural signal and can render it unusable.Read moreRead less
CENTRE for INTEGRATIVE LEGUME RESEARCH. Legumes are essential for environmental sustainability and are important for maintaining human health. The Centre combines innovative genomic approaches to investigate the causal phenotypic links required for regulation of legume growth. The unique coexistence of multiple pluripotent meristems in shoots, roots, flowers and nodules permits the discovery of new paradigms governing legume architecture, reproductive differentiation and root-nodule developmen ....CENTRE for INTEGRATIVE LEGUME RESEARCH. Legumes are essential for environmental sustainability and are important for maintaining human health. The Centre combines innovative genomic approaches to investigate the causal phenotypic links required for regulation of legume growth. The unique coexistence of multiple pluripotent meristems in shoots, roots, flowers and nodules permits the discovery of new paradigms governing legume architecture, reproductive differentiation and root-nodule development. New knowledge of the plant growth processes through mechanistic analysis of organ induction provides the tools to optimise the legume's productivity, quality, and environment adaptation.Read moreRead less