Genetic dissection of a regulatory deubiquitlyation network. The potential impact of this work is widespread, because although it is known that ubiquitlyation has regulatory consequences in multicellular eukaryotes, individual networks have not been completely described in higher eukaryotes. Knowledge gained about fundamental processes in the A. nidulans model system is directly applicable to fungi used in biotechnology in the food, beverage, enzyme and pharmaceutical production industries, and ....Genetic dissection of a regulatory deubiquitlyation network. The potential impact of this work is widespread, because although it is known that ubiquitlyation has regulatory consequences in multicellular eukaryotes, individual networks have not been completely described in higher eukaryotes. Knowledge gained about fundamental processes in the A. nidulans model system is directly applicable to fungi used in biotechnology in the food, beverage, enzyme and pharmaceutical production industries, and to fungal pathogens. Since the fungal genes that form the basis of this project are conserved in higher eukaryotes including humans, the knowledge will be transferable to these systems. A further benefit that cannot be overstated is the research education and training opportunities provided.
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Development and regulation of thermogenesis in thermoregulating flowers. Flowers of certain primitive plants produce enough heat to raise their temperatures up to 40 C above the air, and regulate it at a nearly constant level. Like warm-blooded mammals, the flowers increase heat production as environmental temperature falls. However, they thermoregulate on a cellular level, unlike mammals with their complex nervous system. We aim to elucidate the mechanisms involved in regulation of heat-prod ....Development and regulation of thermogenesis in thermoregulating flowers. Flowers of certain primitive plants produce enough heat to raise their temperatures up to 40 C above the air, and regulate it at a nearly constant level. Like warm-blooded mammals, the flowers increase heat production as environmental temperature falls. However, they thermoregulate on a cellular level, unlike mammals with their complex nervous system. We aim to elucidate the mechanisms involved in regulation of heat-production, with molecular, biochemical and stable isotope techniques. We will investigate spatial and temporal patterns of gene expression and activity of putative regulatory enzymes. The results will have implications for human physiology and agriculture.Read moreRead less
Small is beautiful: Did gene-rich regions of mammal chromosomes evolve from microchromosomes? Most birds and reptile genomes feature many tiny microchromosomes. These are not junk, as previously thought, but contain most of the genes. Mammals lack microchromosomes, but contain gene-rich regions with similar attributes. We suggest that microchromosomes originated by genome duplication, and evolved into the gene-rich regions of mammalian chromosomes. We will test this hypothesis by comparing seque ....Small is beautiful: Did gene-rich regions of mammal chromosomes evolve from microchromosomes? Most birds and reptile genomes feature many tiny microchromosomes. These are not junk, as previously thought, but contain most of the genes. Mammals lack microchromosomes, but contain gene-rich regions with similar attributes. We suggest that microchromosomes originated by genome duplication, and evolved into the gene-rich regions of mammalian chromosomes. We will test this hypothesis by comparing sequences and genes in microchromosomes of birds, reptiles and monotremes. This will clarify the origin and evolution of the ?microgenome?, establish its suitability as a model for vertebrate genome organisation, and demonstrate whether microchromosomes are the ancestors of the gene-rich regions of mammalian chromosomes.Read moreRead less
Microgenomics - a tool to dissect effects of salinity on gene expression in specific cell types of Arabidopsis and rice. This project will provide novel, fundamental understanding of the cell type-specific processes involved in salinity tolerance in higher plants. As such, it will impact on our understanding of a range of processes relevant to salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The increased understanding arising from this ....Microgenomics - a tool to dissect effects of salinity on gene expression in specific cell types of Arabidopsis and rice. This project will provide novel, fundamental understanding of the cell type-specific processes involved in salinity tolerance in higher plants. As such, it will impact on our understanding of a range of processes relevant to salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The increased understanding arising from this project will underpin future work to increase agricultural productivity and the quality of life for all in the Australian and international communities.Read moreRead less
Calcium compartmentation in leaves: testing an integrated model of water and calcium transport with cell specific functional genomics. Calcium is a vital nutrient to animals and humans and its storage in vegetation is important for its accessibility. We believe this storage is linked to water flow in the leaf by a novel mechanism. This project will provide fundamental understanding of the cell type-specific processes involved in calcium storage and water flow in plants. High calibre PhD and Hono ....Calcium compartmentation in leaves: testing an integrated model of water and calcium transport with cell specific functional genomics. Calcium is a vital nutrient to animals and humans and its storage in vegetation is important for its accessibility. We believe this storage is linked to water flow in the leaf by a novel mechanism. This project will provide fundamental understanding of the cell type-specific processes involved in calcium storage and water flow in plants. High calibre PhD and Honours students will be educated to maintain the momentum of international excellence within Australia in the field of plant nutrient relations. The increase in understanding will allow future work to improve calcium availability and water use by plants to the benefit of agricultural productivity and quality of life.Read moreRead less
A study of the nongenomic action of Vitamin D: proposed role of the nuclear VDR and downstream signalling molecules. Vitamin D (1,25D) activates genes in the nucleus through the vitamin D receptor (VDR). 1,25D can also elicit rapid responses at the plasma membrane. This action is critical to the activation of nuclear genes. We hypothesise that a proportion of the nuclear VDR is located at the plasma membrane where it stimulates downstream signalling molecules eg Ras, ERK1/2 and ERK5. We plan to ....A study of the nongenomic action of Vitamin D: proposed role of the nuclear VDR and downstream signalling molecules. Vitamin D (1,25D) activates genes in the nucleus through the vitamin D receptor (VDR). 1,25D can also elicit rapid responses at the plasma membrane. This action is critical to the activation of nuclear genes. We hypothesise that a proportion of the nuclear VDR is located at the plasma membrane where it stimulates downstream signalling molecules eg Ras, ERK1/2 and ERK5. We plan to explore this hypothesis and to identify the signalling molecules. We will also investigate our novel finding that a specific Ras isoform is involved in ERK5 activation. The work will provide new information on signalling pathways.Read moreRead less
The origins of electroreception and nocturnality in the earliest known jawed vertebrates and their bearing on vertebrate diversification. This project aims to discover primary new data to pinpoint the timing, anatomical origins and phylogenetic significance when two key sensory systems first appeared in modern vertebrates: electroreception and specialised nocturnal vision. Such abilities today allow high diversity of vertebrates to co-exist within the same geographical range, for example on trop ....The origins of electroreception and nocturnality in the earliest known jawed vertebrates and their bearing on vertebrate diversification. This project aims to discover primary new data to pinpoint the timing, anatomical origins and phylogenetic significance when two key sensory systems first appeared in modern vertebrates: electroreception and specialised nocturnal vision. Such abilities today allow high diversity of vertebrates to co-exist within the same geographical range, for example on tropical reefs or rainforest communities, through careful temporal niche partitioning where reliance on other sensory systems takes over from vision and olfaction as the principal method of prey detection. This project aims to elucidate how the modern fish diversity was shaped by such significant early evolutionary events.Read moreRead less
Defining New Building Blocks for the Construction of Artificial Genetic Circuits. By characterising the components of a natural genetic switch, we will make available a set of well defined genetic building blocks for construction of rationally designed biological circuits. The ability to build such circuits would have significant economic benefit in areas such as metabolic engineering, to improve the efficiency of production of natural compounds from micro-organisms, and in biomedicine, for the ....Defining New Building Blocks for the Construction of Artificial Genetic Circuits. By characterising the components of a natural genetic switch, we will make available a set of well defined genetic building blocks for construction of rationally designed biological circuits. The ability to build such circuits would have significant economic benefit in areas such as metabolic engineering, to improve the efficiency of production of natural compounds from micro-organisms, and in biomedicine, for the controlled release of therapeutic compounds. The involvement of Honours and Ph.D students in this project will expose the next generation of Australian scientists to this emerging discipline. International collaboration leading to publications in high impact scientific journals will enhance Australia's scientific reputation.Read moreRead less
Tree-mediated methane fluxes: A new frontier in the global carbon cycle. Methane is an extremely potent greenhouse gas. Recent evidence suggests that tree-mediated fluxes may be a significant, but overlooked source of methane to the atmosphere. This project aims to quantify the magnitude and drivers of tree-mediated methane fluxes from Australia’s dominant forest types. Innovatively, we will be using a novel combination of empirical field based measurements, gas tracer experiments, microbial ana ....Tree-mediated methane fluxes: A new frontier in the global carbon cycle. Methane is an extremely potent greenhouse gas. Recent evidence suggests that tree-mediated fluxes may be a significant, but overlooked source of methane to the atmosphere. This project aims to quantify the magnitude and drivers of tree-mediated methane fluxes from Australia’s dominant forest types. Innovatively, we will be using a novel combination of empirical field based measurements, gas tracer experiments, microbial analysis and modelling methods. Expected outcomes are a mechanistic understanding of tree-mediated methane fluxes, helping to constrain regional, national and global methane budgets. The results of this study will help inform publicly funded greenhouse gas abatement strategies, ensuring a maximal return on investment.Read moreRead less
Single Minded 2: Cross coupling or specificity within the bHLH/PAS transcription factor family? Understanding the mechanisms of action of SIM2 may lead to novel ideas towards drug development for diseases such as Down syndrome and cancer. The SIM2 protein can interfere with activity of the related Hypoxia Inducible Factor (HIF), a protein important in stress response and recovery from stroke. Understanding the molecular basis of this interference could aid current strategies being used to manipu ....Single Minded 2: Cross coupling or specificity within the bHLH/PAS transcription factor family? Understanding the mechanisms of action of SIM2 may lead to novel ideas towards drug development for diseases such as Down syndrome and cancer. The SIM2 protein can interfere with activity of the related Hypoxia Inducible Factor (HIF), a protein important in stress response and recovery from stroke. Understanding the molecular basis of this interference could aid current strategies being used to manipulate HIF for pharmaceutical benefit.Read moreRead less