Gating, specificity and regulation of the YggB channel protein from Corynebacterium glutamicum. The proposed research will greatly contribute to our understanding of the functioning of a bacterial membrane channel/transporter, which has played a significant role in biotechnology of commercially important amino acids. A direct national benefit will result from establishing collaboration with a leading German laboratory providing expertise in protein biochemistry and molecular microbiology not ava ....Gating, specificity and regulation of the YggB channel protein from Corynebacterium glutamicum. The proposed research will greatly contribute to our understanding of the functioning of a bacterial membrane channel/transporter, which has played a significant role in biotechnology of commercially important amino acids. A direct national benefit will result from establishing collaboration with a leading German laboratory providing expertise in protein biochemistry and molecular microbiology not available in Australia. The acquired knowledge will present an original contribution which will have a strong impact on a very competitive field of molecular microbiology and biotechnology.Read moreRead less
Particle Design for Recovery and Delivery of Bioactives. This project will develop new strategies for economically viable recovery of bioactives from complex solutions, slurries and sludges of biomaterials eg. waste streams from milk and soy bean processing, and fermentation broths. These bioactives, often proteins, have growing applications as high value drugs, nutriceuticals and food additives but are difficult to separate and to maintain in an active form. Crystallisation will be used as a ....Particle Design for Recovery and Delivery of Bioactives. This project will develop new strategies for economically viable recovery of bioactives from complex solutions, slurries and sludges of biomaterials eg. waste streams from milk and soy bean processing, and fermentation broths. These bioactives, often proteins, have growing applications as high value drugs, nutriceuticals and food additives but are difficult to separate and to maintain in an active form. Crystallisation will be used as a primary separation technique. Molecular studies of protein interactions will be used to predict good crystallisation conditions and linked to process crystallisation studies. Both standard and novel particle design strategies will be used to control crystal size and morphology, as well as package the boactive in a deliverable form without loss of activity.Read moreRead less