The Cytochrome P450 Gene Super-family in Drosophila melanogaster; Gene Function and Insecticide Resistance. The cytochrome P450 (Cyp) gene super-family is represented by over 90 sequences in the genome of the vinegar fly, Drosophila melanogaster. To date, four Cyp genes are found to be involved in insecticide resistance. The function of the majority of Cyp genes is unknown. This project will investigate the function and regulation of D. melanogaster Cyp genes, linking the fly's genotype to its ....The Cytochrome P450 Gene Super-family in Drosophila melanogaster; Gene Function and Insecticide Resistance. The cytochrome P450 (Cyp) gene super-family is represented by over 90 sequences in the genome of the vinegar fly, Drosophila melanogaster. To date, four Cyp genes are found to be involved in insecticide resistance. The function of the majority of Cyp genes is unknown. This project will investigate the function and regulation of D. melanogaster Cyp genes, linking the fly's genotype to its phenotype. By studying the effects of Cyp genes on fly survival, Cyp gene expression and regulation, and expressing selected Cyp genes in a yeast expression system, we will enhance our understanding of Cyp gene function and evolution.Read moreRead less
Marine urban development: how can ecology inform the design of multifunctional artificial structures? This project aims to experimentally manipulate the design of artificial structures to achieve multipurpose ecological objectives. Artificial structures such as buildings and roads have featured in terrestrial urban landscapes for many years, but have only more recently begun encroaching on aquatic environments. Most marine artificial structures lack the innovative design solutions required to mi ....Marine urban development: how can ecology inform the design of multifunctional artificial structures? This project aims to experimentally manipulate the design of artificial structures to achieve multipurpose ecological objectives. Artificial structures such as buildings and roads have featured in terrestrial urban landscapes for many years, but have only more recently begun encroaching on aquatic environments. Most marine artificial structures lack the innovative design solutions required to mitigate their ecological impacts and provide essential ecosystem services such as pollution abatement. This project will investigate the efficacy of these designs with both classical measures of diversity and structure and novel measures of ecosystem function. The project aims to provide essential information to inform the design of future marine urban developments.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE140101611
Funder
Australian Research Council
Funding Amount
$379,040.00
Summary
Snow, shrub and climate feedbacks: impacts of shrub expansion in the Australian alpine zone. This project aims to understand the mechanisms promoting shrub expansion in alpine areas and the consequences of a shrub-dominated landscape in terms of shrubs as hydrological mediators and as biodiversity and ecosystem modifiers. Some shrub species trap wind-blown snow, thereby facilitating seedling survival through soil insulation and increases to meltwater. However, if adaptive and plastic responses t ....Snow, shrub and climate feedbacks: impacts of shrub expansion in the Australian alpine zone. This project aims to understand the mechanisms promoting shrub expansion in alpine areas and the consequences of a shrub-dominated landscape in terms of shrubs as hydrological mediators and as biodiversity and ecosystem modifiers. Some shrub species trap wind-blown snow, thereby facilitating seedling survival through soil insulation and increases to meltwater. However, if adaptive and plastic responses to climate change allows, shrub expansion will have significant negative impacts on alpine biodiversity and ecosystem function. This project will tease apart the interacting effects of snow, recruitment and adaptation to provide models of shrub increase and determine how shrubs modify alpine ecosystem processes and upper catchment hydrology.Read moreRead less
Defining Genetic And Epigenetic Variation During Early Development
Funder
National Health and Medical Research Council
Funding Amount
$996,075.00
Summary
We all began life with a set of genes inherited from our parents. However, it's now known that from the time we were in the womb onwards that genes can be turned off and on by the environment or even completely lost or gained. Even what your mother ate or how she behaved while she was pregnant could have influenced your future health. Because people are so different, we are studying the subtle differences between twins to tease out the factors that may influence our genes and our health.
Temperature sensitivity of soil respiration and its components. This project aims to demonstrate how temperate evergreen forests could buffer against climate change. Soil respiration returns around half the carbon taken up by forests to the atmosphere. This project will characterise and quantify how microbes and roots in soils depend on temperature and substrate supply, and so predict how rising temperatures and drought will affect forests as natural carbon sequestration sinks. This project will ....Temperature sensitivity of soil respiration and its components. This project aims to demonstrate how temperate evergreen forests could buffer against climate change. Soil respiration returns around half the carbon taken up by forests to the atmosphere. This project will characterise and quantify how microbes and roots in soils depend on temperature and substrate supply, and so predict how rising temperatures and drought will affect forests as natural carbon sequestration sinks. This project will resolve the roles of environmental drivers of soil respiration across forests; integrate mechanistic understanding of differing plant and microbial responses to temperature within a common modelling framework; and evaluate the implications of this knowledge in predictions of climatic impacts on terrestrial carbon cycling.Read moreRead less
Epigenetic Programming Of Immune Development In Utero: Role Of The Maternal Environment In The Allergy Epidemic
Funder
National Health and Medical Research Council
Funding Amount
$764,463.00
Summary
This study will provide new insights into the development of allergic disease. Specifically, we will explore the hypothesis that allergic disease and other disorders or immune dysregulation occur as a result of gene-environmental interactions in early life, and that these events begin in pregnancy when the developing fetus is still developing and most susceptible to these effects.
Ecosystem resilience of Shark Bay under changing ocean climate. This project aims to investigate the resilience of the Shark Bay World Heritage Site to projected climate change. This project will generate new knowledge for marine conservation through analyses of habitat loss on nutrient budgets and productivity in seagrass and microbialite ecosystems. Expected outcomes are an improved understanding of climate-driven shifts on ecosystem processes in Shark Bay, incorporating science-based evidence ....Ecosystem resilience of Shark Bay under changing ocean climate. This project aims to investigate the resilience of the Shark Bay World Heritage Site to projected climate change. This project will generate new knowledge for marine conservation through analyses of habitat loss on nutrient budgets and productivity in seagrass and microbialite ecosystems. Expected outcomes are an improved understanding of climate-driven shifts on ecosystem processes in Shark Bay, incorporating science-based evidence for better conservation and management. This will provide significant benefits by contributing to the future-proofing of Shark Bay’s World Heritage values to climate change, and more broadly by demonstrating the consequences of the continued tropicalisation of Australia’s coastline.Read moreRead less
ARC Centre for Kangaroo Genome. In this Australian-led Kangaroo Genome Project, we will map and characterize the tammar wallaby genome at the molecular level. Marsupial genomes are uniquely valuable because they provide comparisons that reveal new human genes, regulatory sequences and marsupial-specific genes. These will deliver new products and information useful for medicine, industry, agriculture and conservation. We will construct integrated genetic and physical maps of the genome, clone the ....ARC Centre for Kangaroo Genome. In this Australian-led Kangaroo Genome Project, we will map and characterize the tammar wallaby genome at the molecular level. Marsupial genomes are uniquely valuable because they provide comparisons that reveal new human genes, regulatory sequences and marsupial-specific genes. These will deliver new products and information useful for medicine, industry, agriculture and conservation. We will construct integrated genetic and physical maps of the genome, clone the whole genome as large inserts in BAC vectors, and build a "golden path" with minimal overlap. We will construct libraries of expressed genes from tammar tissues and array them for use in analysing gene expression.Read moreRead less
Development Of Therapeutically Useful Human Artificial Chromosomes For Gene Delivery And Optimal Gene Expression
Funder
National Health and Medical Research Council
Funding Amount
$496,986.00
Summary
Gene therapy is an exciting new form of treatment for genetic disorders aimed at providing long-term correction of the problems at source - namely the affected gene. The biggest technical hurdle facing gene therapy is to be able to deliver the therapeutic genes efficiently and safely into patient cells. Many gene therapy protocols are currently being trialled clinically. These protocols, based mostly on the use of attenuated viruses to deliver the genes, carry potential risks to the patients in ....Gene therapy is an exciting new form of treatment for genetic disorders aimed at providing long-term correction of the problems at source - namely the affected gene. The biggest technical hurdle facing gene therapy is to be able to deliver the therapeutic genes efficiently and safely into patient cells. Many gene therapy protocols are currently being trialled clinically. These protocols, based mostly on the use of attenuated viruses to deliver the genes, carry potential risks to the patients in terms of infection, immune response, and germline modification. We have developed the first stage of a new technology for gene delivery that does not require the use of viruses. This technology is based on the generation of human artificial chromosomes, which are smaller versions of the naturally occurring chromosomes that carry all the genes inside our cells. Safety in these artificial chromosomes comes from the use of entirely human materials for their engineering. These artificial chromosomes also have other advantages over the viral approaches, including allowing large genes to be carried, and providing a permanent cure in a single treatment. We have already successfully constructed, published, and patented a number of first-generation human artificial chromosomes. The current project aims to complete the next proof-of-concept milestone towards the further development of this technology. Specifically, we propose to demonstrate the ability of the artificial chromosomes to carry genes and provide sustainable expression of these genes in cells and in animal models. Success in this study will allow the technology to proceed rapidly into commercialisation and clinical trial as a new improved tool for gene delivery and gene therapy.Read moreRead less
Cascading effects of Australia's ecological extinctions on biodiversity and ecosystem function. The current rate of species extinctions is so extensive that it has been described as the “sixth mass extinction”. In Australian ecosystems, extinctions and declines of mammals have been dramatic, with formerly abundant species now “ecologically extinct”, meaning they are too rare to continue to play important ecological roles. The loss of entire functional guilds may have cascading effects on biodive ....Cascading effects of Australia's ecological extinctions on biodiversity and ecosystem function. The current rate of species extinctions is so extensive that it has been described as the “sixth mass extinction”. In Australian ecosystems, extinctions and declines of mammals have been dramatic, with formerly abundant species now “ecologically extinct”, meaning they are too rare to continue to play important ecological roles. The loss of entire functional guilds may have cascading effects on biodiversity and ecosystem function. This project uses a multi-scalar experimental approach to investigate the broader impacts of mammal declines on Australian ecosystems, accounting for interactions with climate. The outcomes will include new insights into the pre-European state of Australian ecosystems and more realistic targets for ecosystem restoration.Read moreRead less