Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs. The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics ....Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs. The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics for use in real world applications. The project has two aspects. First, relatively small directed cDNA-bead libraries will be compared to known low-density cDNA microarrays to validate the technique for utility in gene expression profiling. Secondly, large libraries containing short oligonucleotide sequences will be used for de novo sequencing of a complete transcriptome. Proof-of-concept in either case will pave the way for many genomic applications and catapult the technology to 'blockbuster' status.Read moreRead less
Measurement and imaging of pathogenic and diagnostic iron oxide nanoparticles using proton magnetic resonance. This project is likely to result in new and improved technologies to aid in the management and diagnosis of a range of diseases including iron metabolism disorders such as thalassaemia and neurodegenerative diseases such as Alzheimer's disease. Other aspects of the research may lead to technologies for the early detection of some cancers. The technologies will enhance Australia's intern ....Measurement and imaging of pathogenic and diagnostic iron oxide nanoparticles using proton magnetic resonance. This project is likely to result in new and improved technologies to aid in the management and diagnosis of a range of diseases including iron metabolism disorders such as thalassaemia and neurodegenerative diseases such as Alzheimer's disease. Other aspects of the research may lead to technologies for the early detection of some cancers. The technologies will enhance Australia's international standing in the field of advanced medical imaging and have the potential to be commercialised within the Australian biotechnology sector. During the project, research students will receive high quality multidisciplinary training ensuring the supply of personnel with high-level technical expertise into the future.Read moreRead less
Devices that use Ion Channels. The proposed device would supply the community of researchers in Australia and internationally with new techniques to enable them to quickly and conveniently investigate properties of ion channels and to speed the screening of potential ion channel targets for pharmaceutical hits and leads. In addition, the tethered membrane technology will be developed to fill an unmet need for a quick and biologically relevant test of EMC hazards. This will enhance the science an ....Devices that use Ion Channels. The proposed device would supply the community of researchers in Australia and internationally with new techniques to enable them to quickly and conveniently investigate properties of ion channels and to speed the screening of potential ion channel targets for pharmaceutical hits and leads. In addition, the tethered membrane technology will be developed to fill an unmet need for a quick and biologically relevant test of EMC hazards. This will enhance the science and technology infrastructure within Australia, taking it into original and exciting directions, contribute to training young Australian scientists and students, as well as enhance Australia's competitive position in the field of nanobiotechnology.Read moreRead less
Nanoelectromechanical Mass Spectrometry with Molecular Imaging. This project aims to develop new technology to enable simultaneous measurement of the mass and conformation of single molecules. Mass spectrometry and high-resolution microscopy are independent analytical tools used widely to characterise the chemical and physical properties of molecules. This project aims to develop new technology based on advanced nanoelectromechanical systems that combines the capabilities of these complementary ....Nanoelectromechanical Mass Spectrometry with Molecular Imaging. This project aims to develop new technology to enable simultaneous measurement of the mass and conformation of single molecules. Mass spectrometry and high-resolution microscopy are independent analytical tools used widely to characterise the chemical and physical properties of molecules. This project aims to develop new technology based on advanced nanoelectromechanical systems that combines the capabilities of these complementary instruments. This would enable synchronous measurement of molecular mass and conformation with nanometre resolution. In contrast to current mass spectrometry, this technology could be operated in fluid and detect neutral species. This significant change in capability could be applied to advance biological and medical research.Read moreRead less
Advanced high resolution atomic force microscopy of biomolecules in physiological environments. The proposed research is intended to explain high resolution bioimaging problems addressed only phenomenologically before. A correct physical model would help the scientific community to optimise imaging of dynamic biological systems, extending our knowledge about the way living organisms function. With dynamic bioimaging, the mechanism of certain diseases such as Alzheimer's - where biomolecule fiber ....Advanced high resolution atomic force microscopy of biomolecules in physiological environments. The proposed research is intended to explain high resolution bioimaging problems addressed only phenomenologically before. A correct physical model would help the scientific community to optimise imaging of dynamic biological systems, extending our knowledge about the way living organisms function. With dynamic bioimaging, the mechanism of certain diseases such as Alzheimer's - where biomolecule fiber formation plays a key role - can also be addressed, thus the project has even therapeutical relevance. Furthermore, adequate description of liquid phase imaging can help engineers in the design of better hardware and software solutions, for the benefit of the bio-nanotechnological industry.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989615
Funder
Australian Research Council
Funding Amount
$300,000.00
Summary
Melbourne Platform for Surface Characterisation of Structured Materials. The Australian economy is gradually expanding its manufacturing base through the development of the nanotechnology and biotechnology sectors. This will lead to production of a more diverse range of elaborately transformed goods. A key contributor to these export opportunities will be the nanotechnology sector since at the present time no country has a real nanotechnology based economy and there are many niche markets availa ....Melbourne Platform for Surface Characterisation of Structured Materials. The Australian economy is gradually expanding its manufacturing base through the development of the nanotechnology and biotechnology sectors. This will lead to production of a more diverse range of elaborately transformed goods. A key contributor to these export opportunities will be the nanotechnology sector since at the present time no country has a real nanotechnology based economy and there are many niche markets available for smaller countries such as Australia. This proposal helps to build quality control and characterisation infrastructure that will facilitate prototyping and design of nanoscale devices and sensors for next generation manufacturing.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453879
Funder
Australian Research Council
Funding Amount
$184,163.00
Summary
Electron beam induced deposition and ablation nanofabrication facility. Electron beam induced deposition and ablation(EBIDA) is rapidly emerging as a new technology capable of fabricating three-dimensional nanostructures on nearly any substrate with very high precision. This proposal aims to establish a nanoscale EBIDA facility by integrating a specialized nanolithography attachment with an existing state-of-the-art 1nm resolution high current variable pressure scanning electron microscope. This ....Electron beam induced deposition and ablation nanofabrication facility. Electron beam induced deposition and ablation(EBIDA) is rapidly emerging as a new technology capable of fabricating three-dimensional nanostructures on nearly any substrate with very high precision. This proposal aims to establish a nanoscale EBIDA facility by integrating a specialized nanolithography attachment with an existing state-of-the-art 1nm resolution high current variable pressure scanning electron microscope. This combination of instrumentation will enable the high-speed production of conductive and insulating structures with 1-to-10nm dimensions. The unique facility will be used to manufacture and prototype novel nanoscale devices and structures and will enable measurement of their physical and chemical properties.Read moreRead less
Development of direct-write focussed electron beam processing techniques for nano-fabrication applications. The burgeoning disciplines of nanotechnology and biotechnology have the potential to deliver breakthroughs in science and engineering that will revolutionise many aspects of everyday life. Progress in these emerging fields, however, requires parallel advances in the techniques used to fabricate, manipulate and characterise materials and devices at the nanoscale. This project will provide s ....Development of direct-write focussed electron beam processing techniques for nano-fabrication applications. The burgeoning disciplines of nanotechnology and biotechnology have the potential to deliver breakthroughs in science and engineering that will revolutionise many aspects of everyday life. Progress in these emerging fields, however, requires parallel advances in the techniques used to fabricate, manipulate and characterise materials and devices at the nanoscale. This project will provide such enabling tools and fill a major gap in the research infrastructure urgently required by these exciting new technologies.Read moreRead less
Development of a Novel and Quantitative Approach to Phase Imaging with Applications to Functional Nanomaterials. This project will improve and apply an innovative approach to obtaining phase information from electron microscopy images, currently being commercialised by IATIA Ltd. We will develop the approach so that it is fully quantitative, even at the nanoscale, and explore the effect of experimental parameters such as beam coherence, aberrations, specimen contamination and diffraction. We wil ....Development of a Novel and Quantitative Approach to Phase Imaging with Applications to Functional Nanomaterials. This project will improve and apply an innovative approach to obtaining phase information from electron microscopy images, currently being commercialised by IATIA Ltd. We will develop the approach so that it is fully quantitative, even at the nanoscale, and explore the effect of experimental parameters such as beam coherence, aberrations, specimen contamination and diffraction. We will apply the method to both physical and molecular nanomaterials, including a new class of self-organising molecules. Phase imaging can visualise the structures, polarities, charge and conductivity distributions in these materials and so assist in the development of new materials and devices.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0454166
Funder
Australian Research Council
Funding Amount
$1,305,029.00
Summary
Nanoscale Materials Characterization Facility. We request a transmission and a scanning electron microscope, each with specialist electron probes smaller than a nanometre, which can selectively analyse the atomic structure and chemistry of sub-nanometre regions of material.
These capabilities are essential to advance a large range of research projects at the cutting-edge of materials science and engineering, undertaken by Victoria's leading research institutions: five Victorian universities, ....Nanoscale Materials Characterization Facility. We request a transmission and a scanning electron microscope, each with specialist electron probes smaller than a nanometre, which can selectively analyse the atomic structure and chemistry of sub-nanometre regions of material.
These capabilities are essential to advance a large range of research projects at the cutting-edge of materials science and engineering, undertaken by Victoria's leading research institutions: five Victorian universities, the CSIRO, Nanotechnology Victoria Ltd, the Victorian Centre for Advanced Materials Manufacturing and the CRC for Microtechnology. Together they have contributed $2.58 million to this project.
This state-of-the-art facility will include the highest spatial resolution microscope in Australia.
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