DNA Replication fork processing and recovery in living Escherichia coli cells. DNA is the genetic blueprint for all life. When cells divide their DNA has to be copied completely, and exactly, to avoid mutations or death. When the process of copying breaks down, the DNA needs to be repaired and the process of copying restarted. This project will investigate living cells, to understand the mechanisms and pathways involved.
Intersections between science and law: the implementation of threatened species legislation in Australia. This project will evaluate the effectiveness of techniques currently used in threatened species legislation in Australia. By combining the skills of experts in law and ecology, we will assess the role of science and scientists in decision-making processes and the extent to which decision-makers apply the precautionary principle when faced with scientific uncertainty. The project will analy ....Intersections between science and law: the implementation of threatened species legislation in Australia. This project will evaluate the effectiveness of techniques currently used in threatened species legislation in Australia. By combining the skills of experts in law and ecology, we will assess the role of science and scientists in decision-making processes and the extent to which decision-makers apply the precautionary principle when faced with scientific uncertainty. The project will analyse the existing legislative techniques and possible alternatives in the context of the current theoretical debate about the respective advantages of reflexive law (which facilitates self-regulation) and top-down command-and-control regulation.Read moreRead less
Old genes learning new tricks: characterising regulatory changes driving increased heart complexity during vertebrate evolution. The heart has dramatically increased in morphological complexity during vertebrate evolution but the molecular basis driving these major changes remains unknown. Using comparative genomics approaches, this project will explore changes in the regulation of genes involved in heart formation that lead to changes in cardiac structure. It will elucidate for the first time t ....Old genes learning new tricks: characterising regulatory changes driving increased heart complexity during vertebrate evolution. The heart has dramatically increased in morphological complexity during vertebrate evolution but the molecular basis driving these major changes remains unknown. Using comparative genomics approaches, this project will explore changes in the regulation of genes involved in heart formation that lead to changes in cardiac structure. It will elucidate for the first time the cardiac regulatory repertoire in zebrafish and will compare it with that of fly and mouse using cutting-edge bioinformatics pipelines. This work will unravel cardiac-specific regulatory modifications that give rise to evolutionary changes. On a broader scale, it will shed new light on the role of regulatory innovations over gene innovations in the emergence of new traits.Read moreRead less
Links between DNA replication and chromosome end maintenance. This project aims to increase knowledge of the way in which cells maintain their genomes, including the ends of their chromosomes, to enable their own survival. The ends of chromosomes (telomeres) are essential for survival and proliferation of the cells of most organisms. This project aims to determine the molecular details of a recently discovered link between telomere maintenance and the way cells maintain the integrity of their ge ....Links between DNA replication and chromosome end maintenance. This project aims to increase knowledge of the way in which cells maintain their genomes, including the ends of their chromosomes, to enable their own survival. The ends of chromosomes (telomeres) are essential for survival and proliferation of the cells of most organisms. This project aims to determine the molecular details of a recently discovered link between telomere maintenance and the way cells maintain the integrity of their genome. This is likely to lead to increased understanding of the fundamental biological process of genome maintenance, representing a significant scientific advance. The project expects to have far-reaching implications for biotechnology applications that require the survival of cells.Read moreRead less
Structural domains of beta-tubulin and their role in microtubule dynamics and transport. This study aims to obtain a fundamental understanding of how the structural domains of the cytoskeletal protein beta-tubulin are involved in microtubule structures during cell division and vesicular transport. Using gene-editing technology and coupling this with cell biological approaches and high-resolution cell imaging will enable detailed analysis of the role of beta-tubulin domains in these important cel ....Structural domains of beta-tubulin and their role in microtubule dynamics and transport. This study aims to obtain a fundamental understanding of how the structural domains of the cytoskeletal protein beta-tubulin are involved in microtubule structures during cell division and vesicular transport. Using gene-editing technology and coupling this with cell biological approaches and high-resolution cell imaging will enable detailed analysis of the role of beta-tubulin domains in these important cellular processes. The outcomes will include fundamental new knowledge in cell biology and lead to the development of unique biological models that can be used to understand disease.Read moreRead less
Evolving urban structure, mode choice, travel behaviour and energy consumption: A study of the Sydney planning strategy. The project will analyse the emerging Sydney Strategic Plan which is designed to address excessive car reliance that is claimed to cause inefficient energy use, congestion, adverse health and excessive greenhouse gas emissions. The Project's policy framework will contribute to of an environmentally sustainable Australia and will promote public debate. While other funding will ....Evolving urban structure, mode choice, travel behaviour and energy consumption: A study of the Sydney planning strategy. The project will analyse the emerging Sydney Strategic Plan which is designed to address excessive car reliance that is claimed to cause inefficient energy use, congestion, adverse health and excessive greenhouse gas emissions. The Project's policy framework will contribute to of an environmentally sustainable Australia and will promote public debate. While other funding will have expired, the professional academic network will continue to flourish at spatially oriented workshops and conferences which will provide the means of disseminating the analytical techniques, empirical results and policy analysis from the project, thereby enhancing the research capacities of network members.Read moreRead less
Institutional transitions to sustainable agriculture: An inter-disciplinary analysis of a novel common-property resource governance system. Efforts to overcome the severe degradation of land resources supporting agriculture and human communities continue to be constrained by institutions and social norms. The knowledge needed for a transition to sustainable agricultural landscapes through institutional change is inadequate. A collaboration between specialists in economics, politics, sociology, ....Institutional transitions to sustainable agriculture: An inter-disciplinary analysis of a novel common-property resource governance system. Efforts to overcome the severe degradation of land resources supporting agriculture and human communities continue to be constrained by institutions and social norms. The knowledge needed for a transition to sustainable agricultural landscapes through institutional change is inadequate. A collaboration between specialists in economics, politics, sociology, ecology and soil science at the University of New England and graziers in an upper Murray-Darling subcatchment will analyse the transition to a common-property institution on this subcatchment. It will make a distinctively Australian contribution to international efforts to understand such transitions, and serve as a model for progress towards sustainability elsewhere in Australia.
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Development of a behavioural system of stated choice models: modelling behavioural, pricing and technological opportunities to reduce automobile energy levels. Automobile use is attributed with over 70% of CO2 emissions from the transport sector. This project delivers a new framework to assess the impact of policies to reduce CO2 and other energy sources associated with existing ICE fuel sources and a range of scenarios that involve futures with alternative fuels. The proposed framework will rad ....Development of a behavioural system of stated choice models: modelling behavioural, pricing and technological opportunities to reduce automobile energy levels. Automobile use is attributed with over 70% of CO2 emissions from the transport sector. This project delivers a new framework to assess the impact of policies to reduce CO2 and other energy sources associated with existing ICE fuel sources and a range of scenarios that involve futures with alternative fuels. The proposed framework will radically change the approach used by practitioners in prediction, and provide a way of capturing behavioural responses of car users to new environmental futures, in which price, performance, distribution and maintenance play a crucial role in adopting environmentally friendly fuels and vehicle designs. Australia lacks this behavioural capability.Read moreRead less
The Regulatory Network of Histone Methylating and Demethylating Enzymes. This project aims to discover how cells regulate histone methylation enzymes. This process ultimately affects which genes can be turned on or off inside cells; something which is central to growth and development in all animals, all plants and some microbes. Expected outcomes include new knowledge on the regulation of histone methylation, improved techniques for the study of this process and enhanced capacity for internatio ....The Regulatory Network of Histone Methylating and Demethylating Enzymes. This project aims to discover how cells regulate histone methylation enzymes. This process ultimately affects which genes can be turned on or off inside cells; something which is central to growth and development in all animals, all plants and some microbes. Expected outcomes include new knowledge on the regulation of histone methylation, improved techniques for the study of this process and enhanced capacity for international collaboration. New avenues for the artificial regulation of genes may also emerge for synthetic epigenetics. The project should provide significant new findings for the research community, generate research citations and contribute to a highly skilled workforce by the training of staff and students.
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Designer DNA-binding factors. This project aims to use a natural transcription factor family to enhance the efficiency and functionality of designer DNA-binding factors. Research into the structure and function of zinc finger transcription factors, TAL effectors and CRISPR created designer DNA-binding factors. However, though research has improved the specificity of these factors’ genome-wide binding, their efficacy in regulating the expression of genes requires improvement. Using sequencing, th ....Designer DNA-binding factors. This project aims to use a natural transcription factor family to enhance the efficiency and functionality of designer DNA-binding factors. Research into the structure and function of zinc finger transcription factors, TAL effectors and CRISPR created designer DNA-binding factors. However, though research has improved the specificity of these factors’ genome-wide binding, their efficacy in regulating the expression of genes requires improvement. Using sequencing, the project intends to enhance the efficiency and function of these factors by designing modules to improve the stability of DNA binding and effectiveness in functionally regulating gene expression. The project outcomes could include knowledge enabling the use of genetically engineered DNA-binding proteins to artificially control gene expression, with significant scientific and economic implications.Read moreRead less