Advanced high resolution atomic force microscopy of biomolecules in physiological environments. The proposed research is intended to explain high resolution bioimaging problems addressed only phenomenologically before. A correct physical model would help the scientific community to optimise imaging of dynamic biological systems, extending our knowledge about the way living organisms function. With dynamic bioimaging, the mechanism of certain diseases such as Alzheimer's - where biomolecule fiber ....Advanced high resolution atomic force microscopy of biomolecules in physiological environments. The proposed research is intended to explain high resolution bioimaging problems addressed only phenomenologically before. A correct physical model would help the scientific community to optimise imaging of dynamic biological systems, extending our knowledge about the way living organisms function. With dynamic bioimaging, the mechanism of certain diseases such as Alzheimer's - where biomolecule fiber formation plays a key role - can also be addressed, thus the project has even therapeutical relevance. Furthermore, adequate description of liquid phase imaging can help engineers in the design of better hardware and software solutions, for the benefit of the bio-nanotechnological industry.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989615
Funder
Australian Research Council
Funding Amount
$300,000.00
Summary
Melbourne Platform for Surface Characterisation of Structured Materials. The Australian economy is gradually expanding its manufacturing base through the development of the nanotechnology and biotechnology sectors. This will lead to production of a more diverse range of elaborately transformed goods. A key contributor to these export opportunities will be the nanotechnology sector since at the present time no country has a real nanotechnology based economy and there are many niche markets availa ....Melbourne Platform for Surface Characterisation of Structured Materials. The Australian economy is gradually expanding its manufacturing base through the development of the nanotechnology and biotechnology sectors. This will lead to production of a more diverse range of elaborately transformed goods. A key contributor to these export opportunities will be the nanotechnology sector since at the present time no country has a real nanotechnology based economy and there are many niche markets available for smaller countries such as Australia. This proposal helps to build quality control and characterisation infrastructure that will facilitate prototyping and design of nanoscale devices and sensors for next generation manufacturing.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453879
Funder
Australian Research Council
Funding Amount
$184,163.00
Summary
Electron beam induced deposition and ablation nanofabrication facility. Electron beam induced deposition and ablation(EBIDA) is rapidly emerging as a new technology capable of fabricating three-dimensional nanostructures on nearly any substrate with very high precision. This proposal aims to establish a nanoscale EBIDA facility by integrating a specialized nanolithography attachment with an existing state-of-the-art 1nm resolution high current variable pressure scanning electron microscope. This ....Electron beam induced deposition and ablation nanofabrication facility. Electron beam induced deposition and ablation(EBIDA) is rapidly emerging as a new technology capable of fabricating three-dimensional nanostructures on nearly any substrate with very high precision. This proposal aims to establish a nanoscale EBIDA facility by integrating a specialized nanolithography attachment with an existing state-of-the-art 1nm resolution high current variable pressure scanning electron microscope. This combination of instrumentation will enable the high-speed production of conductive and insulating structures with 1-to-10nm dimensions. The unique facility will be used to manufacture and prototype novel nanoscale devices and structures and will enable measurement of their physical and chemical properties.Read moreRead less
Development of direct-write focussed electron beam processing techniques for nano-fabrication applications. The burgeoning disciplines of nanotechnology and biotechnology have the potential to deliver breakthroughs in science and engineering that will revolutionise many aspects of everyday life. Progress in these emerging fields, however, requires parallel advances in the techniques used to fabricate, manipulate and characterise materials and devices at the nanoscale. This project will provide s ....Development of direct-write focussed electron beam processing techniques for nano-fabrication applications. The burgeoning disciplines of nanotechnology and biotechnology have the potential to deliver breakthroughs in science and engineering that will revolutionise many aspects of everyday life. Progress in these emerging fields, however, requires parallel advances in the techniques used to fabricate, manipulate and characterise materials and devices at the nanoscale. This project will provide such enabling tools and fill a major gap in the research infrastructure urgently required by these exciting new technologies.Read moreRead less
Development of a Novel and Quantitative Approach to Phase Imaging with Applications to Functional Nanomaterials. This project will improve and apply an innovative approach to obtaining phase information from electron microscopy images, currently being commercialised by IATIA Ltd. We will develop the approach so that it is fully quantitative, even at the nanoscale, and explore the effect of experimental parameters such as beam coherence, aberrations, specimen contamination and diffraction. We wil ....Development of a Novel and Quantitative Approach to Phase Imaging with Applications to Functional Nanomaterials. This project will improve and apply an innovative approach to obtaining phase information from electron microscopy images, currently being commercialised by IATIA Ltd. We will develop the approach so that it is fully quantitative, even at the nanoscale, and explore the effect of experimental parameters such as beam coherence, aberrations, specimen contamination and diffraction. We will apply the method to both physical and molecular nanomaterials, including a new class of self-organising molecules. Phase imaging can visualise the structures, polarities, charge and conductivity distributions in these materials and so assist in the development of new materials and devices.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0454166
Funder
Australian Research Council
Funding Amount
$1,305,029.00
Summary
Nanoscale Materials Characterization Facility. We request a transmission and a scanning electron microscope, each with specialist electron probes smaller than a nanometre, which can selectively analyse the atomic structure and chemistry of sub-nanometre regions of material.
These capabilities are essential to advance a large range of research projects at the cutting-edge of materials science and engineering, undertaken by Victoria's leading research institutions: five Victorian universities, ....Nanoscale Materials Characterization Facility. We request a transmission and a scanning electron microscope, each with specialist electron probes smaller than a nanometre, which can selectively analyse the atomic structure and chemistry of sub-nanometre regions of material.
These capabilities are essential to advance a large range of research projects at the cutting-edge of materials science and engineering, undertaken by Victoria's leading research institutions: five Victorian universities, the CSIRO, Nanotechnology Victoria Ltd, the Victorian Centre for Advanced Materials Manufacturing and the CRC for Microtechnology. Together they have contributed $2.58 million to this project.
This state-of-the-art facility will include the highest spatial resolution microscope in Australia.
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Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0882821
Funder
Australian Research Council
Funding Amount
$750,000.00
Summary
Ion Beam Nanofabrication and Characterisation Facility for Advanced Materials Research. The requested instrumentation is essential to advance a range of activities at the cutting-edge of materials research and in order to maintain word class research activities in Victoria. The instrumentation requested will build on collaborative links and cultivate enhanced usage of existing facilities between partner organisations. The facility will enhance progress in nanotechnology, biotechnology and materi ....Ion Beam Nanofabrication and Characterisation Facility for Advanced Materials Research. The requested instrumentation is essential to advance a range of activities at the cutting-edge of materials research and in order to maintain word class research activities in Victoria. The instrumentation requested will build on collaborative links and cultivate enhanced usage of existing facilities between partner organisations. The facility will enhance progress in nanotechnology, biotechnology and materials sciences, not only training the next generation of researchers to drive these critical areas, but maintaining Australia's track record as an innovator and developer of advanced materials.Read moreRead less
Nanoparticle fluorescent labels as a platform for high throughput data gathering. Recent developments in genomics require gathering of vast amounts of information in a rapid and cost-effective fashion. To this aim we will develop a new fluorescent labelling technology with adequate throughput and high level of multiplexing. We will exploit recent advances in nanoparticle science combined with our own discoveries concerning rare earth ions as fluorescence activators. Our approach promises a combi ....Nanoparticle fluorescent labels as a platform for high throughput data gathering. Recent developments in genomics require gathering of vast amounts of information in a rapid and cost-effective fashion. To this aim we will develop a new fluorescent labelling technology with adequate throughput and high level of multiplexing. We will exploit recent advances in nanoparticle science combined with our own discoveries concerning rare earth ions as fluorescence activators. Our approach promises a combination of great technological simplicity, low fabrication costs, cutting edge performance and gives an additional time-resolved modality. Our fluorescent labels will be field-tested to search for yet unknown microorganisms.Read moreRead less
Quantitative Atom Probe Tomography for Nanostructural Analysis of Materials. The ultimate in microscopy would involve the ability to image and chemically identify every atom or molecule in a specimen. This project involves the development of reconstruction and analysis methodologies for more accurately determining relative atomic positions in atom probe tomography. We will develop a comprehensive, platform-independent approach to enable quantitative atom probe tomography for the Australian resea ....Quantitative Atom Probe Tomography for Nanostructural Analysis of Materials. The ultimate in microscopy would involve the ability to image and chemically identify every atom or molecule in a specimen. This project involves the development of reconstruction and analysis methodologies for more accurately determining relative atomic positions in atom probe tomography. We will develop a comprehensive, platform-independent approach to enable quantitative atom probe tomography for the Australian research community. This development will be used to address current questions on the influence of the chemistry, crystallography, type and dispersion of sub-critical atomic clusters and supra-critical nanoscale precipitates on the strengthening mechanisms in light alloys used for structural applications in transport.Read moreRead less
Development of a light detection system for enhanced scanning electron microscopy imaging of fully hydrated biological specimens at room temperature. This project aims to develop instrumentation and techniques to image fully hydrated cellS for prolonged periods at room temperature in an environmental scanning electron microscope. Multiple fluorescent nano-particle labels will be used to image complex cellular structure and dynamics at ultra high spatial resolution. This innovative bio-imaging te ....Development of a light detection system for enhanced scanning electron microscopy imaging of fully hydrated biological specimens at room temperature. This project aims to develop instrumentation and techniques to image fully hydrated cellS for prolonged periods at room temperature in an environmental scanning electron microscope. Multiple fluorescent nano-particle labels will be used to image complex cellular structure and dynamics at ultra high spatial resolution. This innovative bio-imaging technique will be facilitated by the construction of a high speed light collection system that can efficiently operate at the pressures required to stablise water within the cell. Applications for this new bio-imaging technique will also be explored.Read moreRead less