Visualising molecular level detail in single cells and intact tissues. The goal of this project is to deliver a new toolkit for imaging cells at an unprecedented resolution and level of chemical detail. We will expand the capabilities of two existing, but complementary, methods: optical fluorescence microscopy with responsive probes and X-ray fluorescence imaging. Expected outcomes include improved techniques and benchmarks for visualising bacterial and mammalian cells; development of new molecu ....Visualising molecular level detail in single cells and intact tissues. The goal of this project is to deliver a new toolkit for imaging cells at an unprecedented resolution and level of chemical detail. We will expand the capabilities of two existing, but complementary, methods: optical fluorescence microscopy with responsive probes and X-ray fluorescence imaging. Expected outcomes include improved techniques and benchmarks for visualising bacterial and mammalian cells; development of new molecules for elucidating cellular chemistry; better utilisation of valuable synchrotron resources; and greater understanding of the strengths and limitations of current microscopy workflows. Results should benefit the biotechnology sector, and may lead to improved medical, diagnostic, and bioremediation capacity.Read moreRead less
Carbon conundrum: Functional characterisation of organic matter-clay mineral interactions in relation to carbon sequestration. Carbon sequestration in soil has been recognised as one of the possible measures through which greenhouse gas emissions can be mitigated. The major processes involved in carbon sequestration in soil include chemical immobilisation of carbon with soil particles and physical protection in the pores of soil microaggregates. These two processes are mediated through the funct ....Carbon conundrum: Functional characterisation of organic matter-clay mineral interactions in relation to carbon sequestration. Carbon sequestration in soil has been recognised as one of the possible measures through which greenhouse gas emissions can be mitigated. The major processes involved in carbon sequestration in soil include chemical immobilisation of carbon with soil particles and physical protection in the pores of soil microaggregates. These two processes are mediated through the functional relationships of soil organic matter and clay mineral interactions in soils. This project investigates nanoscale organomineral association underlying microaggregate formation and stability, as well as the distribution and microbial decomposition of carbon within microaggregates using a suite of advanced spectroscopic, molecular and isotopic techniques.Read moreRead less
Characterisation of the beneficial vs toxic forms of selenium in the diet. This project aims to examine how dietary selenium is converted into essential proteins and beneficial compounds that mitigate against a broad range of human diseases; or alternatively, into toxic molecules. Cutting-edge methodologies should resolve significant unknowns in selenium metabolism, to provide definitive dietary guidelines and to explore how selenium can treat and protect against disease. Expected outcomes from ....Characterisation of the beneficial vs toxic forms of selenium in the diet. This project aims to examine how dietary selenium is converted into essential proteins and beneficial compounds that mitigate against a broad range of human diseases; or alternatively, into toxic molecules. Cutting-edge methodologies should resolve significant unknowns in selenium metabolism, to provide definitive dietary guidelines and to explore how selenium can treat and protect against disease. Expected outcomes from this national and international collaboration include expert training for young biochemical researchers and refinements to novel analytical techniques. Results should benefit the food and agricultural sectors to provide tailored products locally and for export, as well as enhanced health opportunities for all Australians.Read moreRead less