Understanding disease resistance gene evolution across the Brassicaceae. Pan genomes represent the diversity of a species, including structural and sequence variation, which cannot be provided by a reference genome alone. In this project we will characterise resistance gene diversity across the Brassicaceae pan genomes. Through comparison with resistance gene diversity in cultivated Brassica species we will understand selection underlying resistance gene evolution in wild species and subsequent ....Understanding disease resistance gene evolution across the Brassicaceae. Pan genomes represent the diversity of a species, including structural and sequence variation, which cannot be provided by a reference genome alone. In this project we will characterise resistance gene diversity across the Brassicaceae pan genomes. Through comparison with resistance gene diversity in cultivated Brassica species we will understand selection underlying resistance gene evolution in wild species and subsequent domestication and breeding. Knowledge on how variation affects disease susceptibility, especially to the devastating fungal pathogen blackleg, and contributes to phenotypic variation, will lead to improved plant protection strategies and increased crop resilience.Read moreRead less
The More the Merrier? Investigating copy number variation in Brassicas. This project intends to develop an understanding of how gene copy number variation affects disease susceptibility to help in the design of novel plant protection strategies. Gene copy number variants (CNVs) are segments of DNA that have been duplicated or lost in the genome of one individual or line with respect to another. CNVs have been shown to contribute significantly to phenotypic differences in humans, including diseas ....The More the Merrier? Investigating copy number variation in Brassicas. This project intends to develop an understanding of how gene copy number variation affects disease susceptibility to help in the design of novel plant protection strategies. Gene copy number variants (CNVs) are segments of DNA that have been duplicated or lost in the genome of one individual or line with respect to another. CNVs have been shown to contribute significantly to phenotypic differences in humans, including disease susceptibility, and the same seems to apply in plants. This project aims to apply the genome sequences for Brassica species to detect CNVs from re-sequencing data. Knowing how this variation affects an individual or line’s disease susceptibility, especially to the devastating fungal pathogen blackleg, could improve plant protection strategies and crop production.Read moreRead less
Proteome mapping of the model fungal plant pathogen Stagonospora nodorum using LC-LC-MS/MS. Stagonospora nodorum is a fungus that causes leaf and glume blotch disease on wheat. This disease alone causes $55 million dollars in yield losses per annum in Australia. This project aims to identify the proteins produced by Stagonospora nodorum through the development of a new proteomics technique. Two clear benefits to the community resulting from this project will emerge. The first will be the expert ....Proteome mapping of the model fungal plant pathogen Stagonospora nodorum using LC-LC-MS/MS. Stagonospora nodorum is a fungus that causes leaf and glume blotch disease on wheat. This disease alone causes $55 million dollars in yield losses per annum in Australia. This project aims to identify the proteins produced by Stagonospora nodorum through the development of a new proteomics technique. Two clear benefits to the community resulting from this project will emerge. The first will be the expert training of a student in proteomics, a skill that is keenly sought. Secondly, the identification of these pathogen proteins will lead to new strategies to better control the disease and secure the supply of wheat.Read moreRead less
Detection and elimination of resting spores of Olpidium vectoring lettuce big-vein disease in lettuce seedling nursery production. This project will devise nucleic acid and serology methods for detection of Olpidium brassicae, vector of lettuce big-vein disease (LBVD), during various phases of lettuce seedling production in nursery environments. It will be the first study to develop sustainable and environmentally responsible nursery best-practice protocols for integrated management of O. brass ....Detection and elimination of resting spores of Olpidium vectoring lettuce big-vein disease in lettuce seedling nursery production. This project will devise nucleic acid and serology methods for detection of Olpidium brassicae, vector of lettuce big-vein disease (LBVD), during various phases of lettuce seedling production in nursery environments. It will be the first study to develop sustainable and environmentally responsible nursery best-practice protocols for integrated management of O. brassicae in routine seedling production and for management within the lettuce industry. These protocols will ensure that the spread of LBVD from contaminated lettuce nurseries to farms is prevented. It will also be a unique study of the establishment of a disease in new land with different soils and agricultural practices.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0230245
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Joint controlled environment facility for research and development in plant biotechnolgy in Western Australia. The aim of this proposal is to establish a high quality, controlled-environment growth facility for plant research in Perth, jointly managed by Murdoch University and the University of Western Australia. This facility is needed urgently to support current research and new initiatives in plant molecular biology and biotechnology. It will focus on the molecular bases of plant growth and ....Joint controlled environment facility for research and development in plant biotechnolgy in Western Australia. The aim of this proposal is to establish a high quality, controlled-environment growth facility for plant research in Perth, jointly managed by Murdoch University and the University of Western Australia. This facility is needed urgently to support current research and new initiatives in plant molecular biology and biotechnology. It will focus on the molecular bases of plant growth and defence against pathogens. Outcomes include enhancement of WA plant research and its application to improved agricultural production that will benefit rural industries and promote exports. It will also facilitate postgraduate training in plant biotechnology and enhance career prospects of graduates.Read moreRead less
FUNCTIONAL GENOMICS OF PLANT MITOCHONDRIA: THEIR ROLE IN DEVELOPMENT, OXIDATIVE STRESS AND PLANT DEFENSE. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in th ....FUNCTIONAL GENOMICS OF PLANT MITOCHONDRIA: THEIR ROLE IN DEVELOPMENT, OXIDATIVE STRESS AND PLANT DEFENSE. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen attack, and in production of reactive oxygen molecules. This proposal seeks to investigate how mitochondria are involved in these processes, using the latest plant genome information. Potential outcomes include crops better able to cope with environmental stress.Read moreRead less
Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across sout ....Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across southern Australia. Benefits include prevention of severe losses in canola from downy mildew, and more viable and sustainable production with less reliance upon fungicides. This research addresses the National Research Priority 'An Environmentally Sustainable Australia' and the Priority Goal of 'Transforming existing industries', and will particularly benefit southern Australian rural communities.Read moreRead less
Race status, resistance mechanisms, and new sources of resistance to Phytophthora clandestina, a major threat to subterranean clover production. Devastating outbreaks of disease caused by Phytophthora clandestina (Phytophthora root rot) since the late 1970s demonstrated the capacity of this disease to impact severely on clover pasture production across southern Australia, particularly as new races of Phytophthora have rapidly emerged to overcome the resistance of all commercial cultivars. The p ....Race status, resistance mechanisms, and new sources of resistance to Phytophthora clandestina, a major threat to subterranean clover production. Devastating outbreaks of disease caused by Phytophthora clandestina (Phytophthora root rot) since the late 1970s demonstrated the capacity of this disease to impact severely on clover pasture production across southern Australia, particularly as new races of Phytophthora have rapidly emerged to overcome the resistance of all commercial cultivars. The proposed research seeks to delineate new races of the pathogen, to identify the histological and biochemical mechanisms by which resistance to Phytophthora root rot is expressed, and to identify new sources of host resistance. This proposed research will enable breeders, for the first time, to incorporate multiple types of resistance and against different races into new host varieties.Read moreRead less
The development of mass spectrometry techniques for mapping post-translational modifications in the wheat pathogen Stagonospora nodorum. The fungus Stagonospora nodorum is a significant pathogen of wheat causing in excess of $100 million dollars in yield losses per annum in Australia. This project will develop new analytical methods that can be used to detect important protein modifications in Stagonospora nodorum with the goal of securing Australia's wheat supply.
Biosynthesis and functions of two phytotoxins in Septoria nodorum blotch. This project aims to investigate how a fungal plant pathogen makes and uses small bioactive molecules to facilitate infection. It will characterise the function of the genes and enzymes involved in the biosynthesis of a light-activated phytotoxic molecule and a potential anti-plant defence molecule found in the pathogenic wheat fungus Parastagonospora nodorum, and investigate their contribution to disease development. Expe ....Biosynthesis and functions of two phytotoxins in Septoria nodorum blotch. This project aims to investigate how a fungal plant pathogen makes and uses small bioactive molecules to facilitate infection. It will characterise the function of the genes and enzymes involved in the biosynthesis of a light-activated phytotoxic molecule and a potential anti-plant defence molecule found in the pathogenic wheat fungus Parastagonospora nodorum, and investigate their contribution to disease development. Expected outcomes include better understanding of plant-microbe interactions, disease management strategies, technologies for identifying biosynthetic pathways in other fungi, and enzyme technology for synthesising molecules. This could lead to new herbicides, biopesticides and drugs.Read moreRead less