Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561169
Funder
Australian Research Council
Funding Amount
$188,000.00
Summary
Facility for multidimensional fractionation of complex biological mixtures. Acquisition of multidimensional fractionation equipment will allow researchers to separate proteins from complex mixtures, and to compare whole protein profiles of multiple samples. This will permit correlation of specific protein changes associated with infection or disease, a major focus of post-genomic programs of research. The equipment will also provide identification of the key differentiating proteins using mini ....Facility for multidimensional fractionation of complex biological mixtures. Acquisition of multidimensional fractionation equipment will allow researchers to separate proteins from complex mixtures, and to compare whole protein profiles of multiple samples. This will permit correlation of specific protein changes associated with infection or disease, a major focus of post-genomic programs of research. The equipment will also provide identification of the key differentiating proteins using minimal material. Numerous world-class projects and researchers will be able to move more rapidly and reliably from crude cell extracts to identifiable markers, and maintain their competitive positions the recognition of key targets in drug design, disease diagnosis and vaccine development.Read moreRead less
Pilot-scale production of therapeutically-active cannabinoids . The Isolation of minor therapeutically-active cannabinoids from cannabis at pilot scale would establish a commercially competitive Australian industry and lead to a superior position in the global marketplace. This project aims to select elite clones from genetically diverse cannabis strains for yield of minor, but therapeutically-active, cannabinoids, and develop a pilot-scale extraction and separation procedure that can be scaled- ....Pilot-scale production of therapeutically-active cannabinoids . The Isolation of minor therapeutically-active cannabinoids from cannabis at pilot scale would establish a commercially competitive Australian industry and lead to a superior position in the global marketplace. This project aims to select elite clones from genetically diverse cannabis strains for yield of minor, but therapeutically-active, cannabinoids, and develop a pilot-scale extraction and separation procedure that can be scaled-up for commercial production. This would contribute to the growth of the agri-biotechnology sector and a skilled multidisciplinary workforce in rural Australia, thus providing significant economic benefit. The novel scale-up procedure has potential for industry adoption to add value to Australian manufacturing.
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Immobilised Lipid Chromatography for Membrane Protein Isolation and Analysis. Current techniques for membrane protein are inadequate for the emerging proteomic challenge, in which approximately 40% of proteins are predicted to be membrane associated. The aim of this proposal is to develop a new approach to purify membrane proteins using our recently-developed immobilised membrane chromatography materials. The present proposal will provide a new high-resolution separation technique that allows is ....Immobilised Lipid Chromatography for Membrane Protein Isolation and Analysis. Current techniques for membrane protein are inadequate for the emerging proteomic challenge, in which approximately 40% of proteins are predicted to be membrane associated. The aim of this proposal is to develop a new approach to purify membrane proteins using our recently-developed immobilised membrane chromatography materials. The present proposal will provide a new high-resolution separation technique that allows isolation and on-line mass analysis of complex mixtures of membrane proteins for subsequent proteomic analysis, high-throughput screening or structural studies and could form the basis for further development of new commercial tools for membrane protein analysis.Read moreRead less
Characterising post-translational modifications in bacterial proteins. This project represents the first global attempt to characterize post-translational modifications in bacterial proteins using the tools of proteomics. Modifications to proteins are key elements in altering their function. In bacteria, modifications are important in cell-cell adhesion, signalling and triggering of the immune response. Characterisation of modified proteins and their sites of modification represents an opportuni ....Characterising post-translational modifications in bacterial proteins. This project represents the first global attempt to characterize post-translational modifications in bacterial proteins using the tools of proteomics. Modifications to proteins are key elements in altering their function. In bacteria, modifications are important in cell-cell adhesion, signalling and triggering of the immune response. Characterisation of modified proteins and their sites of modification represents an opportunity to understand how bacterial cell populations communicate in the environment, as well as aid in understanding pathogenesis in medical, veterinary and food-borne pathogens. Therefore, improved vaccine targets and therapeutics, as well as method-based products, may be generated by this project.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0237784
Funder
Australian Research Council
Funding Amount
$707,000.00
Summary
Hyphenated Separations/Mass Spectrometry Technology for Protein and Natural Product Characterisation. This proposal consolidates the 3 institutions' collaboration on the ACROSS (Australian Centre for Research on Separation Science) initiative, providing fundamental technology for chemical structural analysis of complex samples involving high resolution protein and natural product characterisation. Requested equipment supports complete characterisation of important novel target molecules. GCxGC-T ....Hyphenated Separations/Mass Spectrometry Technology for Protein and Natural Product Characterisation. This proposal consolidates the 3 institutions' collaboration on the ACROSS (Australian Centre for Research on Separation Science) initiative, providing fundamental technology for chemical structural analysis of complex samples involving high resolution protein and natural product characterisation. Requested equipment supports complete characterisation of important novel target molecules. GCxGC-TOFMS technology will validate our newly patented multidimensional separation techniques. Q-TOF-TOFMS technology, novel patented protein prefractionation approaches, and sample handling with high resolution characterisation / identification of new target proteins allows advanced proteomics developments. Proteomics depends critically upon sophisticated MS techniques. These technologies will: ·enhance the capabilities and expertise in these sciences in the SE Australian area; ·ensure the ACROSS initiative achieves internationally competitive research capabilities; ·provide commercial endpoints in fields associated with analysis of proteins, essential oils and other natural (bio)substances.Read moreRead less
Visualising below the tip of the proteome iceberg. Proteomics attempts to understand human biology by examining the protein components of cells and tissues. Unfortunately, currently available technology only allows approximately 10% of the complexity of these cells and tissues to be concurrently investigated. This project will the physical, chemical and functional properties of protein classes for enrichment, as well as improve technologies for protein visualization, identification and character ....Visualising below the tip of the proteome iceberg. Proteomics attempts to understand human biology by examining the protein components of cells and tissues. Unfortunately, currently available technology only allows approximately 10% of the complexity of these cells and tissues to be concurrently investigated. This project will the physical, chemical and functional properties of protein classes for enrichment, as well as improve technologies for protein visualization, identification and characterization. These approaches will allow the scientific community to further mine beneath the surface of the proteomics 'iceberg'.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE210100140
Funder
Australian Research Council
Funding Amount
$275,000.00
Summary
Quarantined ion chromatography mass spectrometry (IC-MS) facility. This proposal seeks to establish a quarantined facility for 'ion chromatography-mass spectrometry', to provide high resolution ion chromatographic and mass spectrometric analytical capability to the environmental, analytical/bioanalyical, and industrial science research communities. The state-of-the-art facility will represent the only quarantined high-resolution IC-MS facility within Australia, and therefore not only support the ....Quarantined ion chromatography mass spectrometry (IC-MS) facility. This proposal seeks to establish a quarantined facility for 'ion chromatography-mass spectrometry', to provide high resolution ion chromatographic and mass spectrometric analytical capability to the environmental, analytical/bioanalyical, and industrial science research communities. The state-of-the-art facility will represent the only quarantined high-resolution IC-MS facility within Australia, and therefore not only support the above communities within Australia, but the potential to facilitate research collaboration internationally, including supporting Australia's leading Antarctic Science programs. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453295
Funder
Australian Research Council
Funding Amount
$369,697.00
Summary
NMR cryosystem for structural and functional biology. State-of-the-art hardware is requested for the 600-MHz NMR spectrometers situated at University of Sydney and UNSW. A cryosystem installed at USyd. will provide a massive boost in productivity and will allow projects previously inaccessible due to excessive turn-around times, or sensitivity or solubility problems to become tractable. This system will provide new opportunities to researchers from USyd., UNSW and ANU, but will restrict the ver ....NMR cryosystem for structural and functional biology. State-of-the-art hardware is requested for the 600-MHz NMR spectrometers situated at University of Sydney and UNSW. A cryosystem installed at USyd. will provide a massive boost in productivity and will allow projects previously inaccessible due to excessive turn-around times, or sensitivity or solubility problems to become tractable. This system will provide new opportunities to researchers from USyd., UNSW and ANU, but will restrict the versatility of the USyd. instrument. The installation of a TBI probe at UNSW will counter this, and provide a REAL network of NMR instruments across NSW and the ACT.Read moreRead less
Identification of Proteins that Regulate Apoptosis Through Interaction With IAPS. Apoptosis is the process by which multicellular organisms eliminate unwanted cells. Identifying proteins involved in cell death regulation is central to our understanding of disease states arising from aberrations in this process. The mammalian protein DIABLO, promotes cell death by interacting with and antagonising inhibitor of apoptosis proteins (IAPS). Given the existence of several IAP regulatory proteins (IRPs ....Identification of Proteins that Regulate Apoptosis Through Interaction With IAPS. Apoptosis is the process by which multicellular organisms eliminate unwanted cells. Identifying proteins involved in cell death regulation is central to our understanding of disease states arising from aberrations in this process. The mammalian protein DIABLO, promotes cell death by interacting with and antagonising inhibitor of apoptosis proteins (IAPS). Given the existence of several IAP regulatory proteins (IRPs) in insects, other mammalian IRPs probably also exist. These may be of equal importance in regulating apoptosis, especially in tissues where DIABLO is not expressed. The main aim of the proposed study is to idenitify and characterise other IRPs in mammalian cells.Read moreRead less
Functional and structural characterisation of Defective embryo and meristems (Dem) proteins involved in plant development. The proposed research will lead to advances in understanding the regulation of plant development, a process impacting on agriculture, environmental management and human health, areas designated as national research priorities. This understanding is required for modifying plant growth and architecture to fit particular environments, for example generating plants with more ext ....Functional and structural characterisation of Defective embryo and meristems (Dem) proteins involved in plant development. The proposed research will lead to advances in understanding the regulation of plant development, a process impacting on agriculture, environmental management and human health, areas designated as national research priorities. This understanding is required for modifying plant growth and architecture to fit particular environments, for example generating plants with more extensive and deeper roots to mine the soil moisture and nutrients to enhance crop productivity in Australia, and maintaining the competitive advantage of Australian agriculture in view of the range of environmental conditions encountered in this country. The project will also contribute to the health of the Australian population through consumable plants in the diet.Read moreRead less