The other half of the G-protein story: Functional analysis of the plant G-protein gamma subunits. It is now established that G-proteins are involved in the transduction of a number of important processes in plants (Cell division, stomata control, defence, light perception, etc). Nevertheless the data accumulated to date is based on the study of one of the two subunits (alpha) of the G-proteins. Almost nothing is known about the role of the second (and independent) subunit: beta-gamma. We will st ....The other half of the G-protein story: Functional analysis of the plant G-protein gamma subunits. It is now established that G-proteins are involved in the transduction of a number of important processes in plants (Cell division, stomata control, defence, light perception, etc). Nevertheless the data accumulated to date is based on the study of one of the two subunits (alpha) of the G-proteins. Almost nothing is known about the role of the second (and independent) subunit: beta-gamma. We will study for the first time the role of the beta-gamma subunit in plants. We will use a comprehensive approach combining biochemical, physiological, phenotypic, genomic and proteomic studies.Read moreRead less
Discovery of novel circular proteins in bacteria, plants and animals: applications in drug design and crop protection. The overall goal is to use innovative methods in chemistry, biochemistry and molecular biology to determine the structures and functions of several families of circular proteins that have exciting pharmaceutical and agricultural applications. These molecules differ from conventional proteins in that they have no ends: their termini are seamlessly joined, thereby making them exce ....Discovery of novel circular proteins in bacteria, plants and animals: applications in drug design and crop protection. The overall goal is to use innovative methods in chemistry, biochemistry and molecular biology to determine the structures and functions of several families of circular proteins that have exciting pharmaceutical and agricultural applications. These molecules differ from conventional proteins in that they have no ends: their termini are seamlessly joined, thereby making them exceptionally stable and resistant to enzyme digestion. The big question is ?How and why does Nature produce circular proteins?" Increasing our knowledge of why circular proteins have evolved will facilitate their applications in drug design and in the development of novel insecticides.Read moreRead less
Discovering the activity of novel CLE peptide hormones that regulate legume nodulation. This project aims to functionally characterise novel peptide hormones that regulate the number of nitrogen-fixing root nodules that legumes form. Findings will enhance the current nodulation model and could help to alleviate our reliance on expensive, often polluting, nitrogen-fertilisers by helping to optimise the nodulation process in agriculture.
Discovery of the systemic regulator of legume nodulation. This project aims to discover the novel, shoot-derived factor that legumes produce to regulate the number of nitrogen-fixing root nodules they form. Outcomes will enhance the current nodulation model and could help optimise the process in agriculture, which would help alleviate current reliance on nitrogen-fertilisers that are expensive and pollute.
Systemic control of nodule proliferation. We aim to clone and characterize the functions of the supernodulation (NTS-1) locus of soybean using positional cloning and functional genomics approaches. Supernodulation fascinatingly results from a mutant Nts-1 gene functioning in the shoot, although the phenotype is expressed as excessive nodule proliferation in the root. The cloned gene will be used to monitor expression changes after inoculation with Bradyrhizobium, treatment with nitrate, nod-fac ....Systemic control of nodule proliferation. We aim to clone and characterize the functions of the supernodulation (NTS-1) locus of soybean using positional cloning and functional genomics approaches. Supernodulation fascinatingly results from a mutant Nts-1 gene functioning in the shoot, although the phenotype is expressed as excessive nodule proliferation in the root. The cloned gene will be used to monitor expression changes after inoculation with Bradyrhizobium, treatment with nitrate, nod-factor, xylem exudates and phytohormones. We will use RT-PCR, in situ hybridisation and reporter gene expression in transgenic plants. Microarray analysis of soybean ESTs (4200 arrayed) will analyse concurrent gene expression changes in both root and shoot.Read moreRead less
Genetic and molecular analysis of long-distance gene silencing in Arabidopsis. Gene silencing is a surveillance mechanism in plants and animals to ensure that all genes are switched on or off at the right time. It is also a defence mechanism against viruses. Perturbation of gene silencing can be a cause of genetic diseases, and conversely, gene silencing has immense potential as a therapeutic tool for correcting genetic diseases and curing viral diseases. When silencing is triggered against a ge ....Genetic and molecular analysis of long-distance gene silencing in Arabidopsis. Gene silencing is a surveillance mechanism in plants and animals to ensure that all genes are switched on or off at the right time. It is also a defence mechanism against viruses. Perturbation of gene silencing can be a cause of genetic diseases, and conversely, gene silencing has immense potential as a therapeutic tool for correcting genetic diseases and curing viral diseases. When silencing is triggered against a gene or virus in plants, genetic signals are transmitted throughout the organism to systemically switch off the specific gene or virus. Expected long-term national/community benefits from understanding gene silencing are wide-ranging, from improved crops through to drugs and gene therapy.Read moreRead less
Molecular dissection of systemic regulation of nodulation in legumes. This project aims to discover and characterise critical new factors that control legume nodule numbers. Legume plants can increase crop productivity and improve agricultural sustainability by forming specialised root nodules that house nitrogen-fixing rhizobia bacteria. The project will use a multidisciplinary approach to identify the elusive Shoot Derived Inhibitor molecule and define its interaction with novel genes, microRN ....Molecular dissection of systemic regulation of nodulation in legumes. This project aims to discover and characterise critical new factors that control legume nodule numbers. Legume plants can increase crop productivity and improve agricultural sustainability by forming specialised root nodules that house nitrogen-fixing rhizobia bacteria. The project will use a multidisciplinary approach to identify the elusive Shoot Derived Inhibitor molecule and define its interaction with novel genes, microRNAs and phytohormones in nodulation control. Findings will considerably enhance the current nodulation models and will benefit strategies to generate new compounds and crop varieties that mitigate fertiliser requirements, improve soil conditions and increase food security.Read moreRead less
CENTRE for INTEGRATIVE LEGUME RESEARCH. Legumes are essential for environmental sustainability and are important for maintaining human health. The Centre combines innovative genomic approaches to investigate the causal phenotypic links required for regulation of legume growth. The unique coexistence of multiple pluripotent meristems in shoots, roots, flowers and nodules permits the discovery of new paradigms governing legume architecture, reproductive differentiation and root-nodule developmen ....CENTRE for INTEGRATIVE LEGUME RESEARCH. Legumes are essential for environmental sustainability and are important for maintaining human health. The Centre combines innovative genomic approaches to investigate the causal phenotypic links required for regulation of legume growth. The unique coexistence of multiple pluripotent meristems in shoots, roots, flowers and nodules permits the discovery of new paradigms governing legume architecture, reproductive differentiation and root-nodule development. New knowledge of the plant growth processes through mechanistic analysis of organ induction provides the tools to optimise the legume's productivity, quality, and environment adaptation.Read moreRead less
Australian Laureate Fellowships - Grant ID: FL180100139
Funder
Australian Research Council
Funding Amount
$2,973,547.00
Summary
Processes of plant growth that impact agriculture and horticulture. The project aims to discover the genes and processes that control plant shoot architecture, which is a major driver of yield in field, horticultural and forestry crops. Shoot branching is the result of the complex interplay of genes, environment and crop management. By investigating cellular processes governing growth and development, as well as physiology and molecular genetics, this project will enhance Australian capacity and ....Processes of plant growth that impact agriculture and horticulture. The project aims to discover the genes and processes that control plant shoot architecture, which is a major driver of yield in field, horticultural and forestry crops. Shoot branching is the result of the complex interplay of genes, environment and crop management. By investigating cellular processes governing growth and development, as well as physiology and molecular genetics, this project will enhance Australian capacity and multidisciplinary innovation. An improved understanding of shoot branching and how it may be manipulated will improve our knowledge of plant sciences that could contribute to agricultural expansion and food security in Australia and internationally.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE200100800
Funder
Australian Research Council
Funding Amount
$415,693.00
Summary
Legume meristem signalling peptides: an untapped niche. This project aims to characterise novel signalling peptides regulating legume stem cell niches to enhance molecular-genetic networks and uncover potential key targets for crop improvement. Legumes represent agricultural sustainability through their decreased fertiliser requirements resulting in reduced carbon and nitrogen footprints. However, their unique gene signalling networks are poorly understood in comparison to traditional cereal cro ....Legume meristem signalling peptides: an untapped niche. This project aims to characterise novel signalling peptides regulating legume stem cell niches to enhance molecular-genetic networks and uncover potential key targets for crop improvement. Legumes represent agricultural sustainability through their decreased fertiliser requirements resulting in reduced carbon and nitrogen footprints. However, their unique gene signalling networks are poorly understood in comparison to traditional cereal crops. The proposed research intends to generate new knowledge in peptide signalling, plant development and legume symbiosis using multidisciplinary techniques. Expected project outcomes will increase understanding of peptide signalling in legume growth and adaption with useful findings for crop enhancement.Read moreRead less