The LINK to Regulating Lysine Levels in Wheat. This project aims to characterise a recently discovered allosteric mechanism called 'Ligand-Induced association by Lysine (K)' (LINK) model. LINK model regulates the function of a key biosynthetic enzyme in bacteria and plants, including agriculturally-important species such as wheat. Also, it represents a highly significant discovery to the field of biochemistry. The expected outcomes of this project include an in-depth understanding of the molecul ....The LINK to Regulating Lysine Levels in Wheat. This project aims to characterise a recently discovered allosteric mechanism called 'Ligand-Induced association by Lysine (K)' (LINK) model. LINK model regulates the function of a key biosynthetic enzyme in bacteria and plants, including agriculturally-important species such as wheat. Also, it represents a highly significant discovery to the field of biochemistry. The expected outcomes of this project include an in-depth understanding of the molecular basis of a new allosteric mechanism for regulating intracellular lysine levels, which in the longer term offers excellent potential to be manipulated for agricultural benefits.Read moreRead less
Molecular mechanisms governing the role of calcium in aluminium toxicity and tolerance in plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis and plasma membrane potential ....Molecular mechanisms governing the role of calcium in aluminium toxicity and tolerance in plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis and plasma membrane potential in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
Characterisation of PQ loop proteins in plants: are they voltage insensitive nonselective cation channels? Millions of hectares of Australian agricultural land are affected by salinity. This results in the loss of hundreds of millions of dollars in revenue each year. The identification of the pathway for the initial influx of Na+ into plants from the soil will be important in helping to develop crop plants that are salt tolerant. This will increase our understanding of the mechanisms of salinity ....Characterisation of PQ loop proteins in plants: are they voltage insensitive nonselective cation channels? Millions of hectares of Australian agricultural land are affected by salinity. This results in the loss of hundreds of millions of dollars in revenue each year. The identification of the pathway for the initial influx of Na+ into plants from the soil will be important in helping to develop crop plants that are salt tolerant. This will increase our understanding of the mechanisms of salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The future applications of this work will increase agricultural productivity and enhance the quality of life for both Australians and the international community.Read moreRead less
Determining how the soluble dietary fibre beta-glucan is made in cereals. This Project aims to define the molecular mechanisms that control the processes involved in the biosynthesis and regulation of mixed linkage glucan, a major soluble dietary fibre in the cell walls of cereal grains. Plant cell walls determine the quality of most plant-based products used in modern human societies, yet the regulatory mechanisms responsible for their modulation are not well understood. Key distinguishing feat ....Determining how the soluble dietary fibre beta-glucan is made in cereals. This Project aims to define the molecular mechanisms that control the processes involved in the biosynthesis and regulation of mixed linkage glucan, a major soluble dietary fibre in the cell walls of cereal grains. Plant cell walls determine the quality of most plant-based products used in modern human societies, yet the regulatory mechanisms responsible for their modulation are not well understood. Key distinguishing features of the Project will be the international, integrative, and multidisciplinary approach towards addressing this major challenge in plant biology and the potential of the fundamental scientific discoveries to benefit end-users in the food, feed and beverage industries.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0775503
Funder
Australian Research Council
Funding Amount
$255,000.00
Summary
Robotics for plant genomics: Increasing throughput in plant genetic analyses. Plant genomics has direct benefit to crop improvement, especially as focussed in the applicants' laboratories. Thus, the Australian agri-food sector will benefit substantially from the acceleration in plant functional genomics that will arise from the installation of the robotics equipment described in the current application, by both underpinning more applied research and also being used directly in crop improvement p ....Robotics for plant genomics: Increasing throughput in plant genetic analyses. Plant genomics has direct benefit to crop improvement, especially as focussed in the applicants' laboratories. Thus, the Australian agri-food sector will benefit substantially from the acceleration in plant functional genomics that will arise from the installation of the robotics equipment described in the current application, by both underpinning more applied research and also being used directly in crop improvement programs such as are based at the Waite Campus. The outputs will include crops with increased tolerance to biotic and abiotic stresses, a reduced dependence on chemical inputs such as fertilisers and improved food quality, with consequent benefits to the environment and human health and nutrition.Read moreRead less
Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, ....Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, cytosolic pH and aluminium uptake across the plasma membrane in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop culti ....Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop cultivars are important part of sustainable farming in Australia. This project will characterise early triggers of Al toxicity in plants, providing a foundation for increasing Al resistance in crop cultivars. Understanding the physiological basis of Al toxicity will lead to improved crop breeding strategies.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100092
Funder
Australian Research Council
Funding Amount
$240,000.00
Summary
A high-throughput protein production and structure facility. Making proteins and studying their structures and properties is a key activity in biotechnology, drug design, food security and bio-nanotechnology. The Protein Production and Structure Facility will provide Western Australian researchers and their international partners with world-class resources to pursue this research for the benefit of all Australians.
Unravelling the secrets of the rhizosphere of crops. Phosphate is one of the most important limiting nutrients for crop growth and production. Plant acquisition of soil phosphate largely depends on root proliferation to accelerate soil exploration, and on phosphate bioavailability mediated by root exudates and rhizosphere microorganisms. Central to this is the need for a better understanding of the complex biogeochemical interfaces in the rhizosphere. This project explores recently developed non ....Unravelling the secrets of the rhizosphere of crops. Phosphate is one of the most important limiting nutrients for crop growth and production. Plant acquisition of soil phosphate largely depends on root proliferation to accelerate soil exploration, and on phosphate bioavailability mediated by root exudates and rhizosphere microorganisms. Central to this is the need for a better understanding of the complex biogeochemical interfaces in the rhizosphere. This project explores recently developed non-destructive imaging, isotope, and metabolism techniques to generate a systematic research tool in tracking rhizosphere interactions and imaging phosphate dynamics from macroscale to nanoscale levels. This study will provide new opportunities to improve crop nutrient use efficiency and crop production.Read moreRead less
Are plants wasting water in the dark? This project aims to measure stomatal conductance to water vapour in the dark in economically important species to understand how conductance is regulated in the dark, and its adaptive significance. Leaves of most plants continue to lose water in the dark because stomata remain open. No photosynthetic carbon fixation can occur in the dark so water-use efficiency is reduced, and this reduction influences crop yield, forest growth, catchment water yield and c ....Are plants wasting water in the dark? This project aims to measure stomatal conductance to water vapour in the dark in economically important species to understand how conductance is regulated in the dark, and its adaptive significance. Leaves of most plants continue to lose water in the dark because stomata remain open. No photosynthetic carbon fixation can occur in the dark so water-use efficiency is reduced, and this reduction influences crop yield, forest growth, catchment water yield and climate feedback. Existing mechanistic models of stomatal conductance will be extended to include responses in the dark, and aim to be used to predict the reduction in potential daytime water loss (which is coupled to carbon gain) due to nocturnal stomatal conductance for crops and forests.Read moreRead less