Discovery Early Career Researcher Award - Grant ID: DE230101081
Funder
Australian Research Council
Funding Amount
$458,238.00
Summary
Developing CRISPR Prime Editing for highly efficient precise gene editing. This project will further develop a recent breakthrough in gene editing technology named CRISPR prime editing to improve its performance in generating specific genome modifications in cells and organisms. This project expects to generate new knowledge regarding optimal strategies for its deployment as well as create novel enhanced versions of the technology. This would significantly enhance our ability to perform precise ....Developing CRISPR Prime Editing for highly efficient precise gene editing. This project will further develop a recent breakthrough in gene editing technology named CRISPR prime editing to improve its performance in generating specific genome modifications in cells and organisms. This project expects to generate new knowledge regarding optimal strategies for its deployment as well as create novel enhanced versions of the technology. This would significantly enhance our ability to perform precise genome modification of organisms and lead to substantial benefits for a vast array of applications in fundamental and applied biology. Future applications will include generating mutations in cells and model organisms for basic research and creating genetically enhanced agricultural animals or plants.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150101863
Funder
Australian Research Council
Funding Amount
$372,000.00
Summary
Strained alkenes as chemical probes for cysteine sulfenic acid. This project aims to introduce strained alkenes as probes for cysteine sulfenic acid, a poorly understood biomarker for oxidative stress. This probe will enable rapid detection of cysteine sulfenic acid and meet an urgent need for tools to map cysteine redox signalling. Moreover, since many enzymes feature a cysteine sulfenic acid at their active site, the strained alkene probes will also serve as useful inhibitor probes of these en ....Strained alkenes as chemical probes for cysteine sulfenic acid. This project aims to introduce strained alkenes as probes for cysteine sulfenic acid, a poorly understood biomarker for oxidative stress. This probe will enable rapid detection of cysteine sulfenic acid and meet an urgent need for tools to map cysteine redox signalling. Moreover, since many enzymes feature a cysteine sulfenic acid at their active site, the strained alkene probes will also serve as useful inhibitor probes of these enzymes. Such inhibitor probes will provide critical information for potential therapeutic applications in human conditions associated with oxidative stress such as ageing, cancer, and heart disease.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE210100604
Funder
Australian Research Council
Funding Amount
$436,600.00
Summary
How do cells sense and react to mechanical forces? There is accumulating evidence that mechanical forces exerted on tissues and cells strongly influences their behaviour. My research aims to understand how cells sense and respond to forces experienced throughout life. Using a combination of three-dimensional cell and tissue culture methods, I will investigate how compressive forces change the biochemistry of cells and their functionality. This work is aimed at generating fundamental knowledge to ....How do cells sense and react to mechanical forces? There is accumulating evidence that mechanical forces exerted on tissues and cells strongly influences their behaviour. My research aims to understand how cells sense and respond to forces experienced throughout life. Using a combination of three-dimensional cell and tissue culture methods, I will investigate how compressive forces change the biochemistry of cells and their functionality. This work is aimed at generating fundamental knowledge to improve our comprehension of how cells respond to force. The expected outcome is a greater understanding of mechanical and biochemical relationships between cells and the environment, to inform fields of tissue engineering of culture scaffolds to better mimic natural cell-tissue settings.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE160100620
Funder
Australian Research Council
Funding Amount
$378,000.00
Summary
Mechanisms of controlled gene expression in cells and organisms. The goal of this project is to reveal the nature of a cellular mechanism that has a major influence on gene expression in all eukaryotic cells. How gene expression is controlled is of fundamental importance to all life forms. The project plans to develop molecular tools that enable the visualisation and interrogation of this gene regulatory mechanism in live cells, tissues and whole organisms. The outcomes are anticipated to lead t ....Mechanisms of controlled gene expression in cells and organisms. The goal of this project is to reveal the nature of a cellular mechanism that has a major influence on gene expression in all eukaryotic cells. How gene expression is controlled is of fundamental importance to all life forms. The project plans to develop molecular tools that enable the visualisation and interrogation of this gene regulatory mechanism in live cells, tissues and whole organisms. The outcomes are anticipated to lead to an essential understanding of how cells respond to physiological and environmental cues by coordinating changes in gene expression, and to provide potential avenues towards manipulation for pharmaceutical, agricultural and biotechnology purposes.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE180100784
Funder
Australian Research Council
Funding Amount
$365,058.00
Summary
Improving cereal grain quality using epigenetic regulators. The project aims to determine the epigenetic regulatory mechanisms that control cereal grain quality and yield under water-deficit and heat stress. The project will use next-generation sequencing to identify key epigenetic regulators and their functional target genes, which confer superior grain quality to elite genotypes under adverse environments. Project outcomes will benefit cereal breeding by providing more-tailored screening stra ....Improving cereal grain quality using epigenetic regulators. The project aims to determine the epigenetic regulatory mechanisms that control cereal grain quality and yield under water-deficit and heat stress. The project will use next-generation sequencing to identify key epigenetic regulators and their functional target genes, which confer superior grain quality to elite genotypes under adverse environments. Project outcomes will benefit cereal breeding by providing more-tailored screening strategies and superior parental germplasm with enhanced quality and yield. The development of nutritionally improved crops will benefit the Australian cereal industry and export opportunities.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE230100377
Funder
Australian Research Council
Funding Amount
$423,659.00
Summary
Facilitating control of Queensland fruit fly and other insect pests. This project aims to address the need for a Queensland fruit fly male-only sterile release strain for the national Sterile Insect Technique program to control this devastating Australian horticulture pest. By combining two molecular technologies in a new strain that responds to temperature cues to trigger development of only male flies, this project expects to produce twice as many sterile males for release to mate with wild fe ....Facilitating control of Queensland fruit fly and other insect pests. This project aims to address the need for a Queensland fruit fly male-only sterile release strain for the national Sterile Insect Technique program to control this devastating Australian horticulture pest. By combining two molecular technologies in a new strain that responds to temperature cues to trigger development of only male flies, this project expects to produce twice as many sterile males for release to mate with wild females in fruit fly outbreak areas, preventing production of the next generation. Expected outcomes include significant reduction in production costs and increased efficiency of the national sterile release program, facilitating control of this damaging pest to protect Australia's billion dollar horticultural industry.Read moreRead less