Untangling the matrix of bacterial biofilms. This research aims to use forefront molecular microbiology and biophysical approaches to advance fundamental knowledge on bacterial biofilms. These bacterial clusters are held together by an extracellular matrix comprised of bacterial-derived fibrous protein and the polysaccharide cellulose, which imparts structural integrity and resistance to antimicrobials. The major goals of this project are to dissect how bacteria regulate production of the biofil ....Untangling the matrix of bacterial biofilms. This research aims to use forefront molecular microbiology and biophysical approaches to advance fundamental knowledge on bacterial biofilms. These bacterial clusters are held together by an extracellular matrix comprised of bacterial-derived fibrous protein and the polysaccharide cellulose, which imparts structural integrity and resistance to antimicrobials. The major goals of this project are to dissect how bacteria regulate production of the biofilm matrix, and examine how changes in the composition of the matrix alters its properties, including the penetration of antimicrobial peptides and antibiotics. The outcomes will help address the economic burden of difficult to treat industrial, environmental and biomedical biofilms.Read moreRead less
How bacteria form resistant aggregates and biofilms. This research aims to use interdisciplinary approaches to advance fundamental knowledge on bacterial aggregates and biofilms. These bacterial clusters are a significant problem as they have extraordinary resistance to disinfectants and antibiotics, and currently no effective methods are available to disrupt them. The expected outcomes of this project are to dissect how autotransporters, the most common group of bacterial cell-surface proteins, ....How bacteria form resistant aggregates and biofilms. This research aims to use interdisciplinary approaches to advance fundamental knowledge on bacterial aggregates and biofilms. These bacterial clusters are a significant problem as they have extraordinary resistance to disinfectants and antibiotics, and currently no effective methods are available to disrupt them. The expected outcomes of this project are to dissect how autotransporters, the most common group of bacterial cell-surface proteins, promote aggregation and biofilm formation, and to develop inhibitors that prevent the formation of these damaging bacterial clusters. Ultimately, this new knowledge will help address the increasing economic and social burden of industrial, environmental and biomedical biofilms.Read moreRead less
Unlocking the potential of bacterial polymers by defining key determinants. Sugary structures that coat the surface of some bacteria, known as capsules, can be modified by bacterial viruses (bacteriophage) in the environment. For the bacterial genus Acinetobacter, this influences their use as naturally renewable 'green' biopolymers for remediating environments contaminated with petroleum hydrocarbons. This project aims to characterise crucial capsule polymerase enzymes using a combination of bio ....Unlocking the potential of bacterial polymers by defining key determinants. Sugary structures that coat the surface of some bacteria, known as capsules, can be modified by bacterial viruses (bacteriophage) in the environment. For the bacterial genus Acinetobacter, this influences their use as naturally renewable 'green' biopolymers for remediating environments contaminated with petroleum hydrocarbons. This project aims to characterise crucial capsule polymerase enzymes using a combination of bioinformatics and experimental methodologies to establish how bacteriophage influence Acinetobacter capsules. Outcomes include the development of an innovative genomics pipeline to detect capsule change, improving the use of living bacteria for bioremediation and sustainable rehabilitation of natural ecosystems.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE240100842
Funder
Australian Research Council
Funding Amount
$455,057.00
Summary
Roles of emerging pollutants in spreading antimicrobial resistance. Antimicrobial resistance is a growing global challenge, yet the impact of environmental agents on the spread of antimicrobial resistance is poorly understood. Drawing on my recent findings and a tight integration of a model microbial ecology system, this project aims to investigate the impact of environmental pollutants on the colonisation and spread of antimicrobial resistance in situ ecological communities. This project expect ....Roles of emerging pollutants in spreading antimicrobial resistance. Antimicrobial resistance is a growing global challenge, yet the impact of environmental agents on the spread of antimicrobial resistance is poorly understood. Drawing on my recent findings and a tight integration of a model microbial ecology system, this project aims to investigate the impact of environmental pollutants on the colonisation and spread of antimicrobial resistance in situ ecological communities. This project expects to generate new knowledge at the forefront of research into antimicrobial resistance in a complex ecosystem. The outcomes should provide a deep mechanistic understanding of environmental factors associated with antimicrobial resistance, with applications to antimicrobial resistance risk management for One Health.Read moreRead less
Eradicating bacterial biofilms with nitroxide-antimicrobial hybrids. This project aims to develop new antimicrobials to address the rise of drug-resistant infections and resilient bacterial communities called biofilms. We aim to break new ground in our fundamental knowledge of antimicrobial mechanisms and exploit this understanding by fusing cellular/molecular microbiology and synthetic chemistry approaches. We seek to gain an in-depth understanding of how nitroxides induce bacterial biofilm dis ....Eradicating bacterial biofilms with nitroxide-antimicrobial hybrids. This project aims to develop new antimicrobials to address the rise of drug-resistant infections and resilient bacterial communities called biofilms. We aim to break new ground in our fundamental knowledge of antimicrobial mechanisms and exploit this understanding by fusing cellular/molecular microbiology and synthetic chemistry approaches. We seek to gain an in-depth understanding of how nitroxides induce bacterial biofilm dispersal, which is critical for the discovery of anti-biofilm molecules that do not fail due to resistance development. These breakthroughs should induce a step-change in our ability to reduce the occurrence of biofilm-related infection in fields ranging from medical and veterinary to biotechnology and agriculture.Read moreRead less
Unravelling a novel stress-signalling system in bacteria. This project aims to investigate the cyclic-di-AMP signalling system in industrially important bacteria. The recently discovered cyclic-di-AMP is essential for normal bacterial growth and plays key roles in heat and antibiotic resistance, metabolism and virulence. This project will develop new biological assays to shed light on how bacteria sense and respond to environmental stress. Expected outcomes include a much deeper understanding of ....Unravelling a novel stress-signalling system in bacteria. This project aims to investigate the cyclic-di-AMP signalling system in industrially important bacteria. The recently discovered cyclic-di-AMP is essential for normal bacterial growth and plays key roles in heat and antibiotic resistance, metabolism and virulence. This project will develop new biological assays to shed light on how bacteria sense and respond to environmental stress. Expected outcomes include a much deeper understanding of signalling inputs and outputs. This should lead to benefits such as guiding the improvement of bacterial strains used in food and biochemical biotechnological applications, and may provide the foundation for the development of novel antibiotics.Read moreRead less
Investigations into the antibacterial mechanism of action of cannabidiol. Cannabidiol (CBD) comes from a set of naturally occurring compounds, with a range of applications in mainstream culture. We have recently reported that CBD has excellent antimicrobial properties, with the ability to kill bacteria. This project aims to understand how CBD works by examining CBD-bacterial interactions at a genetic and molecular level. By understanding how CBD acts on and within bacterial cells, we can create ....Investigations into the antibacterial mechanism of action of cannabidiol. Cannabidiol (CBD) comes from a set of naturally occurring compounds, with a range of applications in mainstream culture. We have recently reported that CBD has excellent antimicrobial properties, with the ability to kill bacteria. This project aims to understand how CBD works by examining CBD-bacterial interactions at a genetic and molecular level. By understanding how CBD acts on and within bacterial cells, we can create fundamental new knowledge that could lead to the design of improved analogs of CBD to that can treat bacterial infections. As a much-needed completely new antibiotic class, this will lead to significant benefits, supporting Australia's National Strategy to combat the challenges posed by antimicrobial resistance.Read moreRead less
Investigations into the antibacterial mechanism of action of cannabidiol. This project aims to understand how the compound cannabidiol is able to kill bacteria by examining its interactions with bacteria from a genetic and molecular level. This research is critical, because future development of cannabidiol and design of improved analogs is predicated on knowing how it works. Expected outcomes include the first detailed understanding of how cannabidiol interacts with bacteria. This should lead ....Investigations into the antibacterial mechanism of action of cannabidiol. This project aims to understand how the compound cannabidiol is able to kill bacteria by examining its interactions with bacteria from a genetic and molecular level. This research is critical, because future development of cannabidiol and design of improved analogs is predicated on knowing how it works. Expected outcomes include the first detailed understanding of how cannabidiol interacts with bacteria. This should lead to significant benefits, including high impact publications, additional collaborations with industrial partner Botanix, and a new class of antibiotics to overcome antibiotic resistance.Read moreRead less
Defining the molecular basis for Salmonella persistence. Salmonella infections in animals and humans place significant burdens on the agri-food and healthcare sectors. All mammals and avian species can become chronically infected with Salmonella and such chronic carriage is a reservoir for disease and outbreaks in other animals and humans. Significant gaps in our understanding of Salmonella infection remain, including the molecular mechanisms involved in establishing a chronic carrier state. We ....Defining the molecular basis for Salmonella persistence. Salmonella infections in animals and humans place significant burdens on the agri-food and healthcare sectors. All mammals and avian species can become chronically infected with Salmonella and such chronic carriage is a reservoir for disease and outbreaks in other animals and humans. Significant gaps in our understanding of Salmonella infection remain, including the molecular mechanisms involved in establishing a chronic carrier state. We identified several Salmonella specific genes and subsequent murine studies revealed that a Salmonella mutant lacking these genes is attenuated in mice and especially in the gallbladder. In this project we seek to understand the molecular basis for attenuation and the contribution of each protein to diseaseRead moreRead less
YhcB, a crucial player in the control of bacterial cell envelope biogenesis. All life depends on a cell envelope to enclose the chemical reactions that make life possible. But how do cell envelopes grow? How each component of the cell envelope is incorporated into the envelope at the right amount and in the right time to prevent cell death, has been a longstanding question in bacteriology. Using a unique combination of high through put genetic screens and biochemical approaches, this project wil ....YhcB, a crucial player in the control of bacterial cell envelope biogenesis. All life depends on a cell envelope to enclose the chemical reactions that make life possible. But how do cell envelopes grow? How each component of the cell envelope is incorporated into the envelope at the right amount and in the right time to prevent cell death, has been a longstanding question in bacteriology. Using a unique combination of high through put genetic screens and biochemical approaches, this project will characterise a key regulator of cell envelope growth in Gram-negative bacteria. Knowledge arising from this research will provide insight into a fundamental process in bacteria, will develop new technology to probe protein interactions, and will provide novel avenues to solve infection in plants, humans and animals.Read moreRead less