Interaction of Cryptosporidium lifecycle stages with aquatic biofilm communities. Cryptosporidium is the most common non-viral cause of diarrhoeal disease in humans worldwide, and of increasing significance as a cause of disease in livestock and wildlife. It is one of the most significant waterborne pathogens and a major challenge to the provision of safe drinking water by water utilities. Biofilms are a poorly studied component of Cryptosporidium's ecosystem, and can act as an environmental res ....Interaction of Cryptosporidium lifecycle stages with aquatic biofilm communities. Cryptosporidium is the most common non-viral cause of diarrhoeal disease in humans worldwide, and of increasing significance as a cause of disease in livestock and wildlife. It is one of the most significant waterborne pathogens and a major challenge to the provision of safe drinking water by water utilities. Biofilms are a poorly studied component of Cryptosporidium's ecosystem, and can act as an environmental reservoir of the parasite in water storages and pipes and an unpredictable source of water contamination. This project will investigate the nature of this reservoir and factors that support the parasite's survival with a view to providing information of value in limiting the public health significance of the biofilm reservoir.Read moreRead less
Environmental contamination and pig disease: an Australian microbe evolves. The Australian pig industry produces pork commodities from over 4.75 million pigs per year. Infectious diseases in industrial-scale piggeries can have a devastating effect on pork production, particularly on feed conversion efficiency and growth rates, and can pose downstream environmental contamination and food safety risks. This project aims to assess a current infectious disease problem in pigs by studying a microbe t ....Environmental contamination and pig disease: an Australian microbe evolves. The Australian pig industry produces pork commodities from over 4.75 million pigs per year. Infectious diseases in industrial-scale piggeries can have a devastating effect on pork production, particularly on feed conversion efficiency and growth rates, and can pose downstream environmental contamination and food safety risks. This project aims to assess a current infectious disease problem in pigs by studying a microbe that appears to have uniquely evolved in Australia. These results could inform the rational design of monitoring, prevention and treatment strategies to minimise infection outbreaks in Australian pigs and may result in production benefits to the pork industry, reduced environmental microbial contamination and safer food.Read moreRead less
Understanding disease resistance gene evolution across the Brassicaceae. Pan genomes represent the diversity of a species, including structural and sequence variation, which cannot be provided by a reference genome alone. In this project we will characterise resistance gene diversity across the Brassicaceae pan genomes. Through comparison with resistance gene diversity in cultivated Brassica species we will understand selection underlying resistance gene evolution in wild species and subsequent ....Understanding disease resistance gene evolution across the Brassicaceae. Pan genomes represent the diversity of a species, including structural and sequence variation, which cannot be provided by a reference genome alone. In this project we will characterise resistance gene diversity across the Brassicaceae pan genomes. Through comparison with resistance gene diversity in cultivated Brassica species we will understand selection underlying resistance gene evolution in wild species and subsequent domestication and breeding. Knowledge on how variation affects disease susceptibility, especially to the devastating fungal pathogen blackleg, and contributes to phenotypic variation, will lead to improved plant protection strategies and increased crop resilience.Read moreRead less
The More the Merrier? Investigating copy number variation in Brassicas. This project intends to develop an understanding of how gene copy number variation affects disease susceptibility to help in the design of novel plant protection strategies. Gene copy number variants (CNVs) are segments of DNA that have been duplicated or lost in the genome of one individual or line with respect to another. CNVs have been shown to contribute significantly to phenotypic differences in humans, including diseas ....The More the Merrier? Investigating copy number variation in Brassicas. This project intends to develop an understanding of how gene copy number variation affects disease susceptibility to help in the design of novel plant protection strategies. Gene copy number variants (CNVs) are segments of DNA that have been duplicated or lost in the genome of one individual or line with respect to another. CNVs have been shown to contribute significantly to phenotypic differences in humans, including disease susceptibility, and the same seems to apply in plants. This project aims to apply the genome sequences for Brassica species to detect CNVs from re-sequencing data. Knowing how this variation affects an individual or line’s disease susceptibility, especially to the devastating fungal pathogen blackleg, could improve plant protection strategies and crop production.Read moreRead less
Enhancing biopharmaceuticals: A disruptive bioseparation resin technology. This project aims to develop an innovative and disruptive platform technology for designing and manufacturing tailor-made high-performance bioseparation resins to enhance biopharmaceuticals manufacturing. Bacterial cell factories will be developed to enable biotechnological production of innovative polyester bead-based bioseparation resins, which will revolutionise manufacturing of biopharmaceuticals. Expected outcomes o ....Enhancing biopharmaceuticals: A disruptive bioseparation resin technology. This project aims to develop an innovative and disruptive platform technology for designing and manufacturing tailor-made high-performance bioseparation resins to enhance biopharmaceuticals manufacturing. Bacterial cell factories will be developed to enable biotechnological production of innovative polyester bead-based bioseparation resins, which will revolutionise manufacturing of biopharmaceuticals. Expected outcomes of this project are cost-effective and strongly enhanced approaches for biopharmaceuticals recovery, thereby providing significant benefits to accelerate research and development in early stage discovery and manufacture of biologics, therapeutic proteins and vaccines.Read moreRead less
Genetic selection of artificial polyketides. This project aims to harness the potential of synthetic biology to build cells that can make valuable new derivatives of polyketides. Polyketides are natural products that have proven to be highly effective for use in industry, medicine and agriculture. The technologies developed in this project will provide resources to discover new chemicals, accessible to almost any scientific laboratory. Expected outcomes include the opportunity to put the future ....Genetic selection of artificial polyketides. This project aims to harness the potential of synthetic biology to build cells that can make valuable new derivatives of polyketides. Polyketides are natural products that have proven to be highly effective for use in industry, medicine and agriculture. The technologies developed in this project will provide resources to discover new chemicals, accessible to almost any scientific laboratory. Expected outcomes include the opportunity to put the future of natural product discovery and optimisation in the hands of the wider scientific community, which will provide significant benefits, such as providing new tools for Australian industries.Read moreRead less
Pyruvate provision for mitochondrial respiration in plants. This project aims to generate new knowledge about pyruvate provision for respiration in plants as it is a major pathway of carbon loss from plants. It will address specific gaps in knowledge about how pyruvate is provided to mitochondria for respiration, how channelling of pyruvate is achieved between components in this pathway and it will seek to engineering a new pyruvate supply pathway to change respiratory processes in plants. It wi ....Pyruvate provision for mitochondrial respiration in plants. This project aims to generate new knowledge about pyruvate provision for respiration in plants as it is a major pathway of carbon loss from plants. It will address specific gaps in knowledge about how pyruvate is provided to mitochondria for respiration, how channelling of pyruvate is achieved between components in this pathway and it will seek to engineering a new pyruvate supply pathway to change respiratory processes in plants. It will develop techniques for analysis of metabolic processes in plants and genetic proof for assumptions of how plant respiration works. Benefits will be training of early career researchers, enhanced international reputation of Australian plant science and new approaches to engineer respiratory rate in plants.Read moreRead less
Advancing plant synthetic gene circuit capability, robustness, and use. This project aims to advance our ability to control gene expression in plants using synthetic gene circuits. By expanding the toolkit and optimizing circuit components, we aim to achieve more complex capabilities and robust implementation. Furthermore, we will apply gene circuit technologies to enhance plant frost tolerance. The expected project outcomes include a significant advance in gene circuit capabilities, a better un ....Advancing plant synthetic gene circuit capability, robustness, and use. This project aims to advance our ability to control gene expression in plants using synthetic gene circuits. By expanding the toolkit and optimizing circuit components, we aim to achieve more complex capabilities and robust implementation. Furthermore, we will apply gene circuit technologies to enhance plant frost tolerance. The expected project outcomes include a significant advance in gene circuit capabilities, a better understanding of their behavior in plant cells, and the ability to use them to confer advantageous traits. The benefits of this research include new plant biotechnology tools that will underpin future crop yield improvements, and advances in plant-based pharmaceuticals and materials.Read moreRead less
Investigating a novel signalling pathway for crop improvement. This project will dissect a newly identified signalling pathway in plants that regulates plant water use and carbon gain. It will deploy multiple techniques, including novel biosensors, to understand the links between the metabolism of plants and their environmental responses. The project will build partnerships with scientists at leading international institutions for enhanced outcomes, including access to specialised equipment and ....Investigating a novel signalling pathway for crop improvement. This project will dissect a newly identified signalling pathway in plants that regulates plant water use and carbon gain. It will deploy multiple techniques, including novel biosensors, to understand the links between the metabolism of plants and their environmental responses. The project will build partnerships with scientists at leading international institutions for enhanced outcomes, including access to specialised equipment and upskilling of our scientists. The generation of barley with the latest gene editing techniques aims to produce a non-GM crop with the potential for enhanced root C sequestration, lower water use and improved yield, three key goals for agricultural sustainability in the face of a drying Australian climate.Read moreRead less
Farm Women, Networks and ICTs. Considerable national funds have been spent on brining rural Australians online. In the previous year over half of those living in non-metropolitan Australian accessed the internet. However, little is known about how new technologies are being integrated into the daily lives of rural people. This research moves from quantifying technology take-up in rural Australia and describing the possibilities of what new technologies may offer rural people, to analysing and cr ....Farm Women, Networks and ICTs. Considerable national funds have been spent on brining rural Australians online. In the previous year over half of those living in non-metropolitan Australian accessed the internet. However, little is known about how new technologies are being integrated into the daily lives of rural people. This research moves from quantifying technology take-up in rural Australia and describing the possibilities of what new technologies may offer rural people, to analysing and critiquing the extent to which these possibilities are being realised. It will inform policy on rural and regional technology access and use.Read moreRead less