Controlling the adhesome to regulate cell fate on biomaterials. Mesenchymal stem cell-based tissue engineering practices are hampered worldwide by the lack of appreciation and understanding of the matrix-mediated cues that must be provided during adhesion and spreading to drive cells to definitive tissue end points. This project will address these knowledge deficiencies by combining high throughput array technologies, a set of tailorable self-assembling biomaterials and real-time biosensors to r ....Controlling the adhesome to regulate cell fate on biomaterials. Mesenchymal stem cell-based tissue engineering practices are hampered worldwide by the lack of appreciation and understanding of the matrix-mediated cues that must be provided during adhesion and spreading to drive cells to definitive tissue end points. This project will address these knowledge deficiencies by combining high throughput array technologies, a set of tailorable self-assembling biomaterials and real-time biosensors to rapidly, at high resolution, elucidate how mechanotransductive cues determine the fate choice of mesenchymal stem cells, and furthermore, how to manipulate them with smart biomaterial design to achieve desired outcomes for tissue engineering. Read moreRead less
Hypoxia-mimicking bio-scaffold for skeleton regeneration. The project is to develop bioactive bone grafts to improve bone repair and shorten the recovery time of patients with fractures, degenerative joint diseases, and bone cancer and bone deformities.
Elucidating surface-mediated permissive cues for cellular differentiation. This project will develop a novel biomaterial platform technology that will enable firstly the probing and thereafter the control of the cellular pathways of adult mesenchymal stem cells. These fundamental insights will be translated into novel stem cell culture ware products that will enable clinically relevant, functional tissue repair and regeneration.
Potency and activity of Meso-Endothelial bipotent progenitors in vivo. This project aims to characterise a new stem cell population that can maintain both blood vessels and contribute to a variety of tissues whether fibrous, bone, fat or cartilage. Blood vessels comprise an inner endothelial layer and surrounding mesenchyme, are integral to many organs and constitute a unique system connecting different parts of the body. Despite their importance little is known about how they are maintained and ....Potency and activity of Meso-Endothelial bipotent progenitors in vivo. This project aims to characterise a new stem cell population that can maintain both blood vessels and contribute to a variety of tissues whether fibrous, bone, fat or cartilage. Blood vessels comprise an inner endothelial layer and surrounding mesenchyme, are integral to many organs and constitute a unique system connecting different parts of the body. Despite their importance little is known about how they are maintained and how they contribute to the response to injury. Previous work has described several populations of stem cell capable of self renewal and repletion of the endothelium or the mesenchyme. This project will examine the potency of these different progenitors to give rise to each of these fates in homeostasis but also during sounding and bone formation. This will help define a unique population of stem cells capable of both vascular and mesenchymal repair.Read moreRead less
Understanding the differentiation of the endocardium. The project aims to understand the genetic regulation of endocardial development. The heart is essential for survival, its beat the indicator of life. The endocardium, the heart’s inner lining, is required for signalling during heart development and is a major component of the valves, septa and trabeculae. Despite its indispensable role, little is known about how it forms or develops. This project integrates two complementary approaches that ....Understanding the differentiation of the endocardium. The project aims to understand the genetic regulation of endocardial development. The heart is essential for survival, its beat the indicator of life. The endocardium, the heart’s inner lining, is required for signalling during heart development and is a major component of the valves, septa and trabeculae. Despite its indispensable role, little is known about how it forms or develops. This project integrates two complementary approaches that have identified the earliest marker of endocardial differentiation and devised the method to make endocardium from stem cells. Knowledge from this work will inform future research into growing and regenerating damaged tissue.Read moreRead less
Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family protei ....Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family proteins as specific effectors. This project will address a new paradigm in genetics that is likely to underpin development.Read moreRead less
Progressive collapse resistance of concrete flat plate structures. This project aims to systematically and thoroughly investigate the progressive collapse mechanisms and resistance capacity of concrete flat plate structures. They represent one of the most common construction systems used worldwide in modern days, yet their progressive collapse mechanisms require attention and limited collapse prevention design guidelines are available. The outcomes are expected to broaden and deepen the existing ....Progressive collapse resistance of concrete flat plate structures. This project aims to systematically and thoroughly investigate the progressive collapse mechanisms and resistance capacity of concrete flat plate structures. They represent one of the most common construction systems used worldwide in modern days, yet their progressive collapse mechanisms require attention and limited collapse prevention design guidelines are available. The outcomes are expected to broaden and deepen the existing theoretical framework and knowledge base, prevent injury and loss of life in both new and existing buildings. This is expected ultimately to contribute to the establishment of a set of collapse-resistant design guidelines for further development of relevant Australian and international standards.Read moreRead less
Poro-elastic, single domain model of wave-induced transport and transformation of pollutants in coastal sediments. The sediments in many bays and estuaries in Australia are contaminated by pollutants due to discharge of waste from the river, groundwater or/and ocean outfall. Most previous research has had a multi-domain approach and have ignored the wave-dirven advective flow and effects of soil behaviour in coastal sediment. In this study, we will couple the procedure of pollutant transport ne ....Poro-elastic, single domain model of wave-induced transport and transformation of pollutants in coastal sediments. The sediments in many bays and estuaries in Australia are contaminated by pollutants due to discharge of waste from the river, groundwater or/and ocean outfall. Most previous research has had a multi-domain approach and have ignored the wave-dirven advective flow and effects of soil behaviour in coastal sediment. In this study, we will couple the procedure of pollutant transport near the sediment-water interface by a single domain approach, and link wave-dirven advective flow and contaminant in marine sediments. The research outcomes will provide a better prediction of the transformation behaviour of pollutants in contaminated sediments.Read moreRead less
The response of beams subjected to axial load and lateral soil movements. Beams (piles, soil nails, and pipelines) are not only subjected to axial (vertical, axial and longitudinal)loading, but often withstand simultaneous lateral loading, due to either explicit structural loads, or due to loads induced by movement of the soils in which they are founded. Bridge piles adjacent to an approach embankment are one example. This project will provide experimental evidence to assist with the estimation ....The response of beams subjected to axial load and lateral soil movements. Beams (piles, soil nails, and pipelines) are not only subjected to axial (vertical, axial and longitudinal)loading, but often withstand simultaneous lateral loading, due to either explicit structural loads, or due to loads induced by movement of the soils in which they are founded. Bridge piles adjacent to an approach embankment are one example. This project will provide experimental evidence to assist with the estimation of capacity, axial and shear resistance, the validation of unified solutions for beams subjected to simultaneous lateral soil movements and axial loads. Such experimental evidence and analytical solutions are not currently available. The evidence will also assist with solving a contradictory, existing design principle, which will lead to an economic and efficient design approach.Read moreRead less
Rigorous Three Dimensional Plasticity Solutions for Soil and Rock Slopes. Slope failures and landslides are a persistent cause of economic loss in Australia. Damages resulting from landslides include both property damage and loss of life. One such recent catastrophic slope failure is the landslide that occurred at Thredbo Village in New South Wales in 1997. This monumental landslide resulted in the deaths of 18 people and was considered by the coroner as the worst natural disaster in Australian ....Rigorous Three Dimensional Plasticity Solutions for Soil and Rock Slopes. Slope failures and landslides are a persistent cause of economic loss in Australia. Damages resulting from landslides include both property damage and loss of life. One such recent catastrophic slope failure is the landslide that occurred at Thredbo Village in New South Wales in 1997. This monumental landslide resulted in the deaths of 18 people and was considered by the coroner as the worst natural disaster in Australian history. The primary aim of this research project is to apply recently developed computational tools to better understand 3D slope behaviour and to develop rigorous stability solutions that can be used by design engineers. A better understanding of 3D slope failure will lead to more economic and safer slope designs.Read moreRead less