Selective enrichment of proteins using micro-Gradiflow technology and characterisation of proteins using LC-MS/MS. This project describes crucial areas for the technology development of protein enrichment and fractionation. While current techniques are powerful, the usefulness to identify and characterise proteins of low abundance from massively complex samples in the presence of abundant proteins is limited and requires up to mililitre volumes. We intend to develop the micro-Gradiflow technolog ....Selective enrichment of proteins using micro-Gradiflow technology and characterisation of proteins using LC-MS/MS. This project describes crucial areas for the technology development of protein enrichment and fractionation. While current techniques are powerful, the usefulness to identify and characterise proteins of low abundance from massively complex samples in the presence of abundant proteins is limited and requires up to mililitre volumes. We intend to develop the micro-Gradiflow technology in association with Gradipore as a means of protein fractionation using microlitre volumes of sample. This technology will be applicable to all areas of proteomic research and in particular to the study of cell differentiation. The outcome for Australian industry, our competitiveness both industrial and scientific, and the potential for economic advancement is tremendous.Read moreRead less
Polarity in lymphocytes: Regulation of immune function and cancer. The Fellow will elucidate the mechanisms and consequences of a fundamental new phenomenon in immunology. This is likely to profoundly impact upon approaches to detecting and treating immune-related diseases such as autoimmunity, transplantation, vaccination and immunodeficiencies. The ongoing project and the work that led to our discovery involves interdisciplinary collaborations between physicists and biologists to apply frontie ....Polarity in lymphocytes: Regulation of immune function and cancer. The Fellow will elucidate the mechanisms and consequences of a fundamental new phenomenon in immunology. This is likely to profoundly impact upon approaches to detecting and treating immune-related diseases such as autoimmunity, transplantation, vaccination and immunodeficiencies. The ongoing project and the work that led to our discovery involves interdisciplinary collaborations between physicists and biologists to apply frontier laser technologies to biological studies. These technologies will not only facilitate our elucidation of immune development and function, but will also be made available to other research in Australia and overseas.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453295
Funder
Australian Research Council
Funding Amount
$369,697.00
Summary
NMR cryosystem for structural and functional biology. State-of-the-art hardware is requested for the 600-MHz NMR spectrometers situated at University of Sydney and UNSW. A cryosystem installed at USyd. will provide a massive boost in productivity and will allow projects previously inaccessible due to excessive turn-around times, or sensitivity or solubility problems to become tractable. This system will provide new opportunities to researchers from USyd., UNSW and ANU, but will restrict the ver ....NMR cryosystem for structural and functional biology. State-of-the-art hardware is requested for the 600-MHz NMR spectrometers situated at University of Sydney and UNSW. A cryosystem installed at USyd. will provide a massive boost in productivity and will allow projects previously inaccessible due to excessive turn-around times, or sensitivity or solubility problems to become tractable. This system will provide new opportunities to researchers from USyd., UNSW and ANU, but will restrict the versatility of the USyd. instrument. The installation of a TBI probe at UNSW will counter this, and provide a REAL network of NMR instruments across NSW and the ACT.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453400
Funder
Australian Research Council
Funding Amount
$110,040.00
Summary
The Roboocyte™: a medium-throughput, secondary functional, screening facility. Changes in ion channel function have been implicated in a wide variety of human diseases. For this reason many researchers are studying ion channels to understand how they work and how they can develop new drug treatments. The slowest step in evaluating the biological activity of compounds is testing them against the ion channels and the current technology requires much tedious manual handling and extensive operator e ....The Roboocyte™: a medium-throughput, secondary functional, screening facility. Changes in ion channel function have been implicated in a wide variety of human diseases. For this reason many researchers are studying ion channels to understand how they work and how they can develop new drug treatments. The slowest step in evaluating the biological activity of compounds is testing them against the ion channels and the current technology requires much tedious manual handling and extensive operator expertise. The Roboocyte facility will triple testing productivity by allowing for the rapid and automated screening of large libraries of compounds. Such a facility will be unique to the Southern Hemisphere.Read moreRead less
Development of New Generation Magnetic Circular Dichroism Spectrometers. We are building two Magnetic Circular Dichroism (MCD spectrometers) to meet a rising need in the study of metallo-proteins and other molecules of biological interest. The PhD project described in this application will involve instrumentation design, interfacing and sampling handling development for biological samples. We wish to involve a Lastek, a scientific instrumentation company, in the possible commercial development o ....Development of New Generation Magnetic Circular Dichroism Spectrometers. We are building two Magnetic Circular Dichroism (MCD spectrometers) to meet a rising need in the study of metallo-proteins and other molecules of biological interest. The PhD project described in this application will involve instrumentation design, interfacing and sampling handling development for biological samples. We wish to involve a Lastek, a scientific instrumentation company, in the possible commercial development of aspects of this project.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989504
Funder
Australian Research Council
Funding Amount
$1,400,000.00
Summary
Melbourne Biomolecular NMR Network. The Melbourne Biomolecular Nuclear Magnetic Resonance (NMR) Network will support a broad range of basic research activities with long-term benefits for diagnosis, treatment and prevention of numerous diseases. Metabolomics studies will identify human disease markers and characterise the physiological consequences of pharmaceutical intervention. Reverse chemical genetics approaches will identify new drug candidates and reveal novel mechanisms for regulating t ....Melbourne Biomolecular NMR Network. The Melbourne Biomolecular Nuclear Magnetic Resonance (NMR) Network will support a broad range of basic research activities with long-term benefits for diagnosis, treatment and prevention of numerous diseases. Metabolomics studies will identify human disease markers and characterise the physiological consequences of pharmaceutical intervention. Reverse chemical genetics approaches will identify new drug candidates and reveal novel mechanisms for regulating the activities of target proteins. Structural and dynamics studies will reveal molecular details of drug/candidate action and provide guidance for rational improvements. Overall, the planned research activities will have significant benefits for human health.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0882893
Funder
Australian Research Council
Funding Amount
$260,000.00
Summary
A cryopreparation facility for Western Australia. Western Australia is home to a number of world-leading biological and biomedical research groups and some of the best microscopy facilities in the country. Establishing a world-class cryopreparation facility in WA will enable local researchers to remain at the forefront of their research fields and will serve to attract additional high profile international scientists and students to WA. Significant research advancements in areas such as genetic ....A cryopreparation facility for Western Australia. Western Australia is home to a number of world-leading biological and biomedical research groups and some of the best microscopy facilities in the country. Establishing a world-class cryopreparation facility in WA will enable local researchers to remain at the forefront of their research fields and will serve to attract additional high profile international scientists and students to WA. Significant research advancements in areas such as genetic muscular diseases, nerve regeneration, parasitic infection in humans, crop optimisation and tolerance to environmental conditions, and animal growth and reproduction, will result from the establishment of such a facility. Read moreRead less
New Techniques for Structural Biology and Directed Molecular Evolution. This PhD program will equip an Australian graduate with advanced training in techniques in molecular genetics and protein chemistry that are currently in high demand by the biotechnology industry, and also provide him/her with direct experience of an industrial R&D laboratory environment. Moreover, it will establish a basis for further collaboration between a leading University-based research laboratory and an established R& ....New Techniques for Structural Biology and Directed Molecular Evolution. This PhD program will equip an Australian graduate with advanced training in techniques in molecular genetics and protein chemistry that are currently in high demand by the biotechnology industry, and also provide him/her with direct experience of an industrial R&D laboratory environment. Moreover, it will establish a basis for further collaboration between a leading University-based research laboratory and an established R&D company that will lead to development of new techniques for use in biotechnology in Australia and overseas.Read moreRead less
Elucidating the mechanisms by which Scribble, Discs Large and Lethal Giant Larvae regulate epithelial polarity. Discs Large (Dlg) and Scribble are proteins that regulate cell shape by concentrating at particular regions within the cell and recruiting other proteins to that region. It is thought that the two proteins interact with each other, and with other proteins involved in protein trafficking and cell architecture, but it is not known how these events coordinate to produce a net outcome on ....Elucidating the mechanisms by which Scribble, Discs Large and Lethal Giant Larvae regulate epithelial polarity. Discs Large (Dlg) and Scribble are proteins that regulate cell shape by concentrating at particular regions within the cell and recruiting other proteins to that region. It is thought that the two proteins interact with each other, and with other proteins involved in protein trafficking and cell architecture, but it is not known how these events coordinate to produce a net outcome on cell shape. To answer these questions, the dynamic events involved in localization of Dlg, Scribble, and associated proteins will be determined, in mammalian cells and in whole organs of the vinegar fly.Read moreRead less
Nucleotides for bioelectronics. Bioelectronics is a key component of nanobiotechnology, a new field that merges the science of biological molecules (DNA, proteins etc.) with electronics and semiconductor physics. We have recently introduced redox-labelled nucleotides that enable the production of electrochemically-active DNA and RNA for genetic, forensic and nanotechnology applications. This project further develops redox nucleotides for use in electronic devices.