Proteome Analysis of Plant Response Pathways to Microbial Signals in the Model Legume, Medicago truncatula. This project will investigate plant responses to soil microbes in the model legume, Medicago truncatula, to provide fundamental information needed to design crops with improved abilities to interact beneficially with soil microbes. Plant development and performance are significantly influenced by soil microbes, but it is largely unknown how the information contained in microbial signalling ....Proteome Analysis of Plant Response Pathways to Microbial Signals in the Model Legume, Medicago truncatula. This project will investigate plant responses to soil microbes in the model legume, Medicago truncatula, to provide fundamental information needed to design crops with improved abilities to interact beneficially with soil microbes. Plant development and performance are significantly influenced by soil microbes, but it is largely unknown how the information contained in microbial signalling molecules is relayed to plants. Proteome analysis and immunocytochemistry will be combined to identify and localise differentially expressed proteins in roots treated with specific microbial signal molecules. Annotated Proteome databases will be generated to strengthen and complement an international project on M. truncatula genome analysis.Read moreRead less
Plant Transfer Cells - Discovering the Mechanisms of Wall Ingrowth Formation. This project seeks fundamental molecular understanding of how specialized plant cells that are designed for optimum transport of nutrients develop. So-called "transfer cells" are important for efficient nutrient transport and distribution in many crop species of significance to agriculture. Discovering the mechanisms that coordinate development of these specialized cells will maintain Australia's international reputat ....Plant Transfer Cells - Discovering the Mechanisms of Wall Ingrowth Formation. This project seeks fundamental molecular understanding of how specialized plant cells that are designed for optimum transport of nutrients develop. So-called "transfer cells" are important for efficient nutrient transport and distribution in many crop species of significance to agriculture. Discovering the mechanisms that coordinate development of these specialized cells will maintain Australia's international reputation in this field of research, as well as provide technological opportunities to enhance crop yields by manipulating the efficiency of nutrient distribution in crop species. Read moreRead less
Induction of Plant Transfer Cells - Discovering Regulatory Networks. This project seeks molecular understanding of regulatory mechanisms responsible for inducing formation of specialized plant cells that are of central importance in controlling nutrient transport. These so-called "transfer cells" play pivotal roles in determining crop nutrition and hence yield under normal and stressful environments such as soil nutrient deficiencies and salinity. Discovering regulatory mechanisms that control f ....Induction of Plant Transfer Cells - Discovering Regulatory Networks. This project seeks molecular understanding of regulatory mechanisms responsible for inducing formation of specialized plant cells that are of central importance in controlling nutrient transport. These so-called "transfer cells" play pivotal roles in determining crop nutrition and hence yield under normal and stressful environments such as soil nutrient deficiencies and salinity. Discovering regulatory mechanisms that control formation of these specialized cells will maintain Australia's international reputation in this field of research. In addition, the information platform generated may provide technological opportunities to optimise nutrient flows in healthy plants, combat certain environmental stresses and control pathogen attack.
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Physiological and molecular controls of plant transpiration efficiency: investigating the role of the ERECTA gene. Water is the single most limiting factor in agriculture and the world's supply of fresh water is diminishing, the greatest fraction of total water use being by agriculture. Progress in water-use efficiency will have social value, and this program should help us to achieve it. Our progress in this area is already one of the most successful of 'bottom-up' approaches - in the sense of ....Physiological and molecular controls of plant transpiration efficiency: investigating the role of the ERECTA gene. Water is the single most limiting factor in agriculture and the world's supply of fresh water is diminishing, the greatest fraction of total water use being by agriculture. Progress in water-use efficiency will have social value, and this program should help us to achieve it. Our progress in this area is already one of the most successful of 'bottom-up' approaches - in the sense of transferring knowledge from biochemistry and biophysics to breeding and agronomy, as CSIRO now has a successful wheat breeding program based on this earlier work of ours. Now that we have discovered a gene that controls water-use efficiency at the leaf level, we wish to see how the gene works, and how it affects mineral nutrition of leaves.Read moreRead less
The other half of the G-protein story: Functional analysis of the plant G-protein gamma subunits. It is now established that G-proteins are involved in the transduction of a number of important processes in plants (Cell division, stomata control, defence, light perception, etc). Nevertheless the data accumulated to date is based on the study of one of the two subunits (alpha) of the G-proteins. Almost nothing is known about the role of the second (and independent) subunit: beta-gamma. We will st ....The other half of the G-protein story: Functional analysis of the plant G-protein gamma subunits. It is now established that G-proteins are involved in the transduction of a number of important processes in plants (Cell division, stomata control, defence, light perception, etc). Nevertheless the data accumulated to date is based on the study of one of the two subunits (alpha) of the G-proteins. Almost nothing is known about the role of the second (and independent) subunit: beta-gamma. We will study for the first time the role of the beta-gamma subunit in plants. We will use a comprehensive approach combining biochemical, physiological, phenotypic, genomic and proteomic studies.Read moreRead less
Is the extreme phosphate sensitivity found among Australian plants a consequence of their adaptation to a severely phosphate-limited environment? The phosphorus (P)-impoverished soils of south-western Australia have allowed the evolution of many plants that are amazingly efficient at retrieving P from dying tissues. This project will contribute to the understanding of the mechanism determining P efficiency and will contribute significantly to the development of crops that are less reliant on non ....Is the extreme phosphate sensitivity found among Australian plants a consequence of their adaptation to a severely phosphate-limited environment? The phosphorus (P)-impoverished soils of south-western Australia have allowed the evolution of many plants that are amazingly efficient at retrieving P from dying tissues. This project will contribute to the understanding of the mechanism determining P efficiency and will contribute significantly to the development of crops that are less reliant on non-renewable P fertilisers.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE180100001
Funder
Australian Research Council
Funding Amount
$345,475.00
Summary
Pushing the limits of fluorescence microscopy with adaptive optics. This project aims to establish an adaptive optics, super-resolution optical microscopy facility to image cellular events with the highest possible spatial resolution, in a whole cell or tissue context. Sophisticated computer-controlled deformable mirrors will be used to correct the way light is distorted as it passes through specimens, thereby overcoming aberrations found in thick and complex samples. This adaptive optics system ....Pushing the limits of fluorescence microscopy with adaptive optics. This project aims to establish an adaptive optics, super-resolution optical microscopy facility to image cellular events with the highest possible spatial resolution, in a whole cell or tissue context. Sophisticated computer-controlled deformable mirrors will be used to correct the way light is distorted as it passes through specimens, thereby overcoming aberrations found in thick and complex samples. This adaptive optics system will enable researchers to study complex behaviour of biological specimens, at the optical resolution limit in plant and animal tissues, leading to basic biology and biotechnology outcomes in biofuels, biomaterials and biomedicines.Read moreRead less
Developmental functions of oxygen and redox cues in plants. This project aims to transform our understanding of the regulation of meristem functions, with a central hypothesis that plant cell quiescence (repressed cell division) is governed by oxygen and oxidation/reduction (redox)-dependent cues. Meristems are the growing tips of plants, and thus the fundamental unit of plant growth and productivity. This project will develop new knowledge of how plants integrate changes in the environment to r ....Developmental functions of oxygen and redox cues in plants. This project aims to transform our understanding of the regulation of meristem functions, with a central hypothesis that plant cell quiescence (repressed cell division) is governed by oxygen and oxidation/reduction (redox)-dependent cues. Meristems are the growing tips of plants, and thus the fundamental unit of plant growth and productivity. This project will develop new knowledge of how plants integrate changes in the environment to regulate meristem activity. This project will define new paradigms of how oxygen and redox status interact with energy and other cues to regulate decisions to grow or quiesce. This will underpin the development of new strategies to optimise crop management and productivity, improve the efficiency of inputs, and reduce the risk of decision making in crop production.Read moreRead less
Unsaturation of vapour pressure inside leaves: fundamental, but unknown. This project aims to determine when and to what extent the air inside leaves becomes unsaturated with water vapour. All current interpretation and modelling of leaf gas exchange assumes saturation under all circumstances. Compelling evidence has been obtained that suggests this is not true under moderate air vapour pressure deficits. A novel technique will be employed to assess the water vapour concentration of the air insi ....Unsaturation of vapour pressure inside leaves: fundamental, but unknown. This project aims to determine when and to what extent the air inside leaves becomes unsaturated with water vapour. All current interpretation and modelling of leaf gas exchange assumes saturation under all circumstances. Compelling evidence has been obtained that suggests this is not true under moderate air vapour pressure deficits. A novel technique will be employed to assess the water vapour concentration of the air inside leaves based on stable isotope analysis of carbon dioxide and water vapour exchanged between leaves and air. The project is expected to provide fundamental knowledge about how stomata regulate photosynthesis and water use, with significant implications for modelling vegetation function and for improving the performance of crop plants.Read moreRead less
Advanced solar powered hydrogen production systems based on green algal cells. This project aims to enhance the efficiency of solar powered hydrogen production from water and will facilitate the co-production of H2 and oil through microalgal biofuel systems. This frontier science project will therefore deliver a process with high solar conversion efficiency and will deliver multiple product streams increasing profitability.