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Scheme : NHMRC Project Grants
Research Topic : Synaptic Transmission
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  • Funded Activity

    Synaptic Integration And Plasticity In The Rat Piriform Cortex

    Funder
    National Health and Medical Research Council
    Funding Amount
    $250,500.00
    Summary
    The human cerebral cortex is the pinnacle of evolution. It is the most complex structure known, responsible for all of those skills - like language and reasoning - that make our species so remarkable. It is also a major site of many brain diseases, like schizophrenia and epilepsy. An understanding of how the cerebral cortex works would be a remarkable achievement, of immeasurable benefit to human health. How can one go about studying such a complex structure? The strategy taken in this project i .... The human cerebral cortex is the pinnacle of evolution. It is the most complex structure known, responsible for all of those skills - like language and reasoning - that make our species so remarkable. It is also a major site of many brain diseases, like schizophrenia and epilepsy. An understanding of how the cerebral cortex works would be a remarkable achievement, of immeasurable benefit to human health. How can one go about studying such a complex structure? The strategy taken in this project is to begin by studying one of the simplest regions of the cerebral cortex, the olfactory (or piriform) cortex. The olfactory cortex is an evolutionarily ancient region of cortex, with a simpler architecture than other cortical regions. Its task is to process the sense of smell, a primitive sense that is more elaborated in lower animals than in humans. The broad goal of our research is to understand, by studying the olfactory cortex of rats, how olfactory processing occurs at the level of nerve cells (neurons). We will use a number of powerful techniques - including microelectrode recording and laser microscopy - to measure the electrical properties of individual neurons. We will also study the synaptic connections between neurons, and how these connections change following memory-inducing stimuli. It is hoped that this work will shed light on how the healthy cortex is able to process and store information, and how brain diseases cause these functions to deteriorate.
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    Funded Activity

    IDENTIFICATION AND FUNCTION OF RECEPTORS ON SYMPATHETIC TERMINAL SCHWANN CELLS

    Funder
    National Health and Medical Research Council
    Funding Amount
    $235,500.00
    Summary
    The terminals of sympathetic nerves control many of the internal organs. Pharmacological intervention to promote or antagonize the effects of these terminals is very important in a number of different disease states of the autonomic nervous system. The present research proposal sets out to determine the way in which glial cells that partly envelop these terminals control their capacity to function.
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    Funded Activity

    Sulfonadyn-based Dynamin I-specific Inhibitors And Epilepsy

    Funder
    National Health and Medical Research Council
    Funding Amount
    $835,291.00
    Summary
    Epilepsy affects 1% of people, yet 30% do not respond to anti-epileptic drugs (AEDs). Traditional drug discovery fails to improve this situation. Our team discovered dynamin as a new target for better AED design and our lead sulphonadyns reduces seizures in animals. We will design better sulfonadyns that can ultimately be used for clinical trials by designing the drugs away from its actions outside of neurons. If successful, this will accelerate new AED development with less side-effects.
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    Funded Activity

    The Contributions Of Different Sources Of Calcium To The Induction Of Long Term Potentiation

    Funder
    National Health and Medical Research Council
    Funding Amount
    $267,750.00
    Summary
    When we make memories, we alter the strength of synaptic connections between nerve cells.These changes are particularly marked in the hippopcampus ; a region of the brain involved in the formation of memories. The strength of a synaptic connection is altered if it activates a neurone sufficiently to cause a rise in the level of calcium ions. Calcium can be derived from several sources within the neurone. This project aims to assess the relative importance of these different sources of calcium in .... When we make memories, we alter the strength of synaptic connections between nerve cells.These changes are particularly marked in the hippopcampus ; a region of the brain involved in the formation of memories. The strength of a synaptic connection is altered if it activates a neurone sufficiently to cause a rise in the level of calcium ions. Calcium can be derived from several sources within the neurone. This project aims to assess the relative importance of these different sources of calcium in inducing increases in synaptic strength.
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    Funded Activity

    Modulation And Trafficking Of SK Channels In The Lateral Amygdala

    Funder
    National Health and Medical Research Council
    Funding Amount
    $260,980.00
    Summary
    The amygdala is a brain structure that underlies emotional processing. Malfunctions in emotional processing are thought to be the cause of anxiety disorders. Understanding amygdala physiology is thus vital for developing therapies to treat these disorders. We have recently found a novel role for an ion channel in controlling amygdala excitability. In this grant we will investigate how this ion channel is modulated, which will elucidate a novel way in which activity in the amygdala is regulated.
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    Funded Activity

    Synaptic Inhibition And The Control Of Excitability In The Rodent Piriform Cortex

    Funder
    National Health and Medical Research Council
    Funding Amount
    $459,738.00
    Summary
    We are studying the properties of neurons (nerve cells) and brain circuits that enable mammals to recognise and remember odours. Our experiments will focus on neurons in the odour-processing region of the cerebral cortex of mice. This work will answer fundamental questions about how the brain interprets sensory inputs in order to build a coherent picture of the external world. Our findings will also provide a deeper understanding of the causes of epilepsy, leading to improved treatments.
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    Funded Activity

    Excitability And Hyperexcitability Of Neural Circuits In The Rodent Piriform Cortex

    Funder
    National Health and Medical Research Council
    Funding Amount
    $371,807.00
    Summary
    We are studying the properties of neurons (nerve cells) and brain circuits that enable mammals to recognise and remember odours. Our experiments will focus on neurons in the odour-processing region of the cerebral cortex of mice. This work will answer fundamental questions about how the brain interprets sensory inputs in order to build a coherent picture of the external world. Our findings will also provide a deeper understanding of the causes of epilepsy, leading to improved treatments.
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    Funded Activity

    Vasomotor Ganglionic Transmission: The Preganglionic Peptide And The Second Gear

    Funder
    National Health and Medical Research Council
    Funding Amount
    $451,896.00
    Summary
    Blood pressure depends on nerve signals that travel from the central nervous system to blood vessels. In the middle of this pathway is a relay station - the sympathetic ganglion cell. Transmission through this relay station has recently been shown to have not only a fixed but also a variable component - the 'second gear'. The project tests if and how three likely candidate peptide molecules, one in the nerves, two in the bloodstream, control this 'second gear' and hence regulate blood pressure.
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    Funded Activity

    Dendritic Mechanisms Underlying Synaptic Plasticity

    Funder
    National Health and Medical Research Council
    Funding Amount
    $324,750.00
    Summary
    The research described in this Project Grant application should help to us understand how our brains make memories. Our brains contain billions of interconnected nerve cells forming unimaginable numbers of possible networks. Previous research indicates that repetitive activation of individual networks can lead to changes in the strength of connections between nerve cells. These changes in connection strength are thought to underlie learning and memory. The experiments described in this proposal .... The research described in this Project Grant application should help to us understand how our brains make memories. Our brains contain billions of interconnected nerve cells forming unimaginable numbers of possible networks. Previous research indicates that repetitive activation of individual networks can lead to changes in the strength of connections between nerve cells. These changes in connection strength are thought to underlie learning and memory. The experiments described in this proposal will address the mechanisms underlying changes in the strength of connections between nerve cells. As most of the inputs nerve cells receive from other nerve cells are made onto their dendrites (small branching processes that extend from the cell body), the main objective is to investigate the interactions at the dendritic level responsible for changes in connection strength. The results of this work will raise our understanding of how memories are formed, which will be essential if we are to understand the cellular processes disrupted during memory dysfunction in neurological disorders such as dementia.
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    Funded Activity

    Cellular Mechanisms Underlying Absence Epilepsy

    Funder
    National Health and Medical Research Council
    Funding Amount
    $292,223.00
    Summary
    Absence epilepsy is the commonest form of idiopathic generalized epilepsy. It can lead to hundreds of seizures per day, and mainly affects children between the ages of four and eight. Its cause is in most cases unknown. In this study we will use a rat model of absence epilepsy to investigate the cellular basis of this disease. Preliminary work indicates that a particular protein - HCN1 - is reduced in the cortex of rats with absence epilepsy. This protein codes for a pore in the membrane of nerv .... Absence epilepsy is the commonest form of idiopathic generalized epilepsy. It can lead to hundreds of seizures per day, and mainly affects children between the ages of four and eight. Its cause is in most cases unknown. In this study we will use a rat model of absence epilepsy to investigate the cellular basis of this disease. Preliminary work indicates that a particular protein - HCN1 - is reduced in the cortex of rats with absence epilepsy. This protein codes for a pore in the membrane of nerve cells, which acts like a switch. We have preliminary evidence that in rats with absence epilepsy this switch does not work properly. We wish to investigate how this influences the activity of nerve cells in rats with absence epilepsy. Furthermore, as absence epilepsy is an inherited disease, we wish to track down the genetic basis of this disease. This will give us clues as to the cause of the disease in this rat model. This research will shed light on the potentially important role of the HCN1 protein in absence epilepsy, which may represent an potentially new therapeutic target for the development of drugs for the treatment of absence epilepsy.
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