Nanopore sensors for multiplexed, ultra-fast gene detection. The aim of this research is to develop the application of protein nanopores for multiplex identification of DNA samples for ultrafast gene detection. This is a type of barcoding of organism DNA that allows for rapid gene identification. This technology aims to address a significant need for rapid, on-the-spot identification of organisms. Applications include rapid identification of pathogenic bacteria in infections and identification o ....Nanopore sensors for multiplexed, ultra-fast gene detection. The aim of this research is to develop the application of protein nanopores for multiplex identification of DNA samples for ultrafast gene detection. This is a type of barcoding of organism DNA that allows for rapid gene identification. This technology aims to address a significant need for rapid, on-the-spot identification of organisms. Applications include rapid identification of pathogenic bacteria in infections and identification of organisms in environmental sampling. Current methods are relative slow, require DNA amplification and specialised laboratories.
This proposal aims to fine tune the properties of the large nanopore, polyC9, with respect to size and charge, as well as to identify and characterise novel large nanopores. Read moreRead less
Investigating the structure of a T cell immune checkpoint molecule. This project aims to investigate the basic structure and function of a key co-receptor expressed on T cells, known as lymphocyte activation gene-3. T cells play a role in the immune system but must be managed to prevent autoimmunity. Insight into the function of the lymphocyte activation gene-3 function can be used to tailor immunotherapeutics to treat a variety of diseases, including cancer. Functionality of the T cell recept ....Investigating the structure of a T cell immune checkpoint molecule. This project aims to investigate the basic structure and function of a key co-receptor expressed on T cells, known as lymphocyte activation gene-3. T cells play a role in the immune system but must be managed to prevent autoimmunity. Insight into the function of the lymphocyte activation gene-3 function can be used to tailor immunotherapeutics to treat a variety of diseases, including cancer. Functionality of the T cell receptor is determined by utilising structural biology and cellular immunology techniques. The impact of this project effects the development of innovative T cell immunomodulatory agents, improving the health and quality of life of the Australian population.Read moreRead less
Investigating the atomic structure of an immune cell inhibitory receptor. T cells play a key role in the adaptive immune system, whose reactivity must be controlled to prevent aberrant reactivity. Central to the function of T cells is the T cell antigen receptor, and a host of co-stimulatory molecules, co-receptors and inhibitory receptors. This proposal, in partnership with Immutep Ltd, aims to gain a basic understanding of the structure and function of a key inhibitory receptor found on T cel ....Investigating the atomic structure of an immune cell inhibitory receptor. T cells play a key role in the adaptive immune system, whose reactivity must be controlled to prevent aberrant reactivity. Central to the function of T cells is the T cell antigen receptor, and a host of co-stimulatory molecules, co-receptors and inhibitory receptors. This proposal, in partnership with Immutep Ltd, aims to gain a basic understanding of the structure and function of a key inhibitory receptor found on T cells, termed the Lymphocyte activation gene-3 (LAG-3). The proposal utilises a combination of cellular immunology and structural biology to gain insight into the form and function of the LAG-3 molecule. Ultimately this fundamental knowledge can be used by the biotechnology industry.Read moreRead less
Connecting the dots: Image analysis for single molecule localisation microscopy. It is now possible to record the positions of single fluorescent molecules in intact cells to build up an image literally molecule by molecule. But how to ‘connect the dots’ and extract structural information from molecular coordinates is yet to be worked out. The project aims to do exactly that, and write and implement novel analysis routines to quantify a diverse range of biological structures such as protein comp ....Connecting the dots: Image analysis for single molecule localisation microscopy. It is now possible to record the positions of single fluorescent molecules in intact cells to build up an image literally molecule by molecule. But how to ‘connect the dots’ and extract structural information from molecular coordinates is yet to be worked out. The project aims to do exactly that, and write and implement novel analysis routines to quantify a diverse range of biological structures such as protein complexes, membrane morphologies, filamentous cytoskeletal networks, vesicles and viruses. The project is a collaboration between a cell biologist (Professor Gaus) who is a leader in single molecule localisation microscopy, an expert in fluorescence image analysis (Dr Nicovich) and an industry partner (Dr Lucas) who has a sophisticated software platform.Read moreRead less
Engineering new tools to aid structure determination of membrane proteins. This project aims to address the inherent instability of G protein-coupled receptors (GPCRs), which are cell-surface proteins that are a major drug targets. The instability of GPCRs has resulted in a lack of atomic-level structural information that has hindered structure-based drug discovery efforts. This project expects to develop tools to improve GPCR stability and streamline the structure determination process. Project ....Engineering new tools to aid structure determination of membrane proteins. This project aims to address the inherent instability of G protein-coupled receptors (GPCRs), which are cell-surface proteins that are a major drug targets. The instability of GPCRs has resulted in a lack of atomic-level structural information that has hindered structure-based drug discovery efforts. This project expects to develop tools to improve GPCR stability and streamline the structure determination process. Project outcomes are intended to lead to significant advances in membrane protein structure determination and will have a substantial impact on future research in the pharmaceutical industry.Read moreRead less
Exploring therapeutic approaches to combat Coeliac Disease. In Coeliac disease the immune system reacts to ingested wheat protein and attacks the tissue of the small intestine. The wheat protein activates certain immune cells and this initiates a destructive inflammation. In collaboration with the Australian-based company Nexpep, this project aims to develop therapeutics against Coeliac disease.
Exploring the immunomodulatory potential of Natural Killer T cells. Natural Killer T cells (NKT cells) are an innate-like population of T cells that recognise a range of lipid based antigens when bound to the antigen-presenting molecule, CD1d. Once activated, NKT cells rapidly secrete a variety of cytokines. This project, in partnership with Vaxine Pty Ltd, aims to gain a basic understanding of NKT recognition of lipids and NKT cell activation by lipids. The project aims to use a combination of ....Exploring the immunomodulatory potential of Natural Killer T cells. Natural Killer T cells (NKT cells) are an innate-like population of T cells that recognise a range of lipid based antigens when bound to the antigen-presenting molecule, CD1d. Once activated, NKT cells rapidly secrete a variety of cytokines. This project, in partnership with Vaxine Pty Ltd, aims to gain a basic understanding of NKT recognition of lipids and NKT cell activation by lipids. The project aims to use a combination of cellular immunology and structural biology to gain insight into NKT cell agonism.Read moreRead less
Structure-based design of inhibitors of HIV-1 integrase. This project will produce compounds that block human immunodeficiency virus (HIV) replication. These compounds will benefit the 17000 Australians and more than 34 million people worldwide who are currently suffering with this terrible disease.
Advanced cryobanking for propagation-recalcitrant and critically endangered plant species. This project aims to advance methods for the conservation of recalcitrant/threatened plant species to achieve best practice ecological restoration in areas of high biodiversity. In particular, the nature of freezing and dehydration damage to cell membranes at various stages of tissue culture and cryopreservation will be investigated to try to minimise deleterious effects. In addition, metabolic changes tha ....Advanced cryobanking for propagation-recalcitrant and critically endangered plant species. This project aims to advance methods for the conservation of recalcitrant/threatened plant species to achieve best practice ecological restoration in areas of high biodiversity. In particular, the nature of freezing and dehydration damage to cell membranes at various stages of tissue culture and cryopreservation will be investigated to try to minimise deleterious effects. In addition, metabolic changes that affect cryogenic survival will be assessed to provide further insight into the role of oxidative stress and the toxicity of cryopreservation processes. Ultimately, improved cryogenic protocols will be developed to maintain the integrity of long-term cryobanks of key species for ecological restoration, such as in post-mined areas.Read moreRead less
Enhancing stress tolerance in plants by manipulation of properties of stress responsive transcription factors. This project will describe structural and functional properties of cereal transcription factors that are critical to plant development under hostile environmental conditions. The knowledge of the revealed molecular mechanisms of transcription factors will open avenues to developments of improved crop plants.