Origin of animal sensory and nervous systems: a case study in cell type evolution. The origin of the nerve cell and the nervous system allowed the first animals to interact with their biotic and abiotic environment in rapid and complex ways. These capabilities are the primary agents for success in the animal kingdom, underpinning the ability to capture food, avoid predation, and find a mate. These same nerve cells have bestowed on the human brain cognitive abilities that have driven our biologic ....Origin of animal sensory and nervous systems: a case study in cell type evolution. The origin of the nerve cell and the nervous system allowed the first animals to interact with their biotic and abiotic environment in rapid and complex ways. These capabilities are the primary agents for success in the animal kingdom, underpinning the ability to capture food, avoid predation, and find a mate. These same nerve cells have bestowed on the human brain cognitive abilities that have driven our biological and cultural evolution. Despite the phenomenal importance of the nerve cell, we know almost nothing about its origin and early evolution. This basic research project seeks to make a major contribution towards addressing this gap in knowledge.Read moreRead less
Mass-production of beneficial insects for commercial pest management - physicochemical definition of oviposition sites for development of cost-efficient artificial substrates. The mass-production of beneficial insects for commercial purposes demands the development of cost-effective techniques for breeding and distributing them to growers. Many predatory beneficial insects lay their eggs in sites that prevent effective mass culturing. To circumvent this difficulty, it should be possible to defin ....Mass-production of beneficial insects for commercial pest management - physicochemical definition of oviposition sites for development of cost-efficient artificial substrates. The mass-production of beneficial insects for commercial purposes demands the development of cost-effective techniques for breeding and distributing them to growers. Many predatory beneficial insects lay their eggs in sites that prevent effective mass culturing. To circumvent this difficulty, it should be possible to define such oviposition sites in chemical and physical terms, and then use the information to develop artificial substrates that are convenient for mass rearing the insects and for disseminating them to growers. We will use the mealybug predator Cryptolaemus montrouszieri to test the feasibility of this approach and to assess its application to other beneficial species.Read moreRead less
Understanding the molecular basis of marine invertebrate larval settlement and metamorphosis using complementary molecular, developmental, chemical and ecological approaches. The colonisation and fouling of submerged structures is a major economic problem for maritime industries. This proposal will detail the molecular mechanisms underlying the first crucial steps of marine invertebrate colonisation - settlement and metamorphosis - and determine how natural anti-foulant chemicals impact on these ....Understanding the molecular basis of marine invertebrate larval settlement and metamorphosis using complementary molecular, developmental, chemical and ecological approaches. The colonisation and fouling of submerged structures is a major economic problem for maritime industries. This proposal will detail the molecular mechanisms underlying the first crucial steps of marine invertebrate colonisation - settlement and metamorphosis - and determine how natural anti-foulant chemicals impact on these developmental processes. By using complementary developmental, molecular, chemical and ecological approaches, we will obtain knowledge of key control points in settlement and metamorphosis, and thus identify potential pest management strategies. This proposal comprehensively delineates the role of novel anti-foulants, linking their effect on invertebrate development with their role in shaping marine communities on natural and built surfaces.Read moreRead less
Understanding social cancers: Intra-specific parasitism by honeybee workers. Our project will study the conditions under which normally altruistic honeybee workers parasitise other colonies. Thus we will explore a fundamental question: how is the expression of selfish behaviour normally controlled? Outcomes of this project will be important to our understanding of insect societies but will also have application for those studying the development of tumours in multicellular organisms, the develop ....Understanding social cancers: Intra-specific parasitism by honeybee workers. Our project will study the conditions under which normally altruistic honeybee workers parasitise other colonies. Thus we will explore a fundamental question: how is the expression of selfish behaviour normally controlled? Outcomes of this project will be important to our understanding of insect societies but will also have application for those studying the development of tumours in multicellular organisms, the development of metazoan bodies, and social cohesion in human and non-human societies. Our project will also help protect Australia's honey industry from the devastating social parasites that have ruined the industry in South Africa.Read moreRead less
Regulatory cellular microRNAs and their role in insect anti-viral responses. This project will use cutting edge approaches to reveal fundamental roles of small ribonucleic acid molecules (microRNAs) in insect anti-viral responses and immunity. By manipulating anti-viral immune responses, the project will assist in the design of novel approaches to pest control and abolish/limit transmission of vector-borne viruses such as Dengue virus.
Discovery Early Career Researcher Award - Grant ID: DE120101512
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Investigating the interaction of microRNAs-Wolbachia-Dengue virus in the mosquito vector, Aedes aegypti. This project focuses on using molecular techniques to discover fundamental roles of small RNA molecules (microRNAs) of a key mosquito vector in bacterial symbiosis and Dengue virus infection. It will lead to development of effective approaches in limiting spread of vector and transmission of life threatening viral diseases.
Host provisioning by Wolbachia: re-examining the invasion potential of a common invertebrate endosymbiont. Wolbachia are often described as reproductive parasites that manipulate their host for their own gain. This study will determine for the first time how Wolbachia can help its insect host during periods of nutritional stress. As Wolbachia are able to infect host populations it will be used in the near future to control insects that transmit disease or are commercial pests. Understanding how ....Host provisioning by Wolbachia: re-examining the invasion potential of a common invertebrate endosymbiont. Wolbachia are often described as reproductive parasites that manipulate their host for their own gain. This study will determine for the first time how Wolbachia can help its insect host during periods of nutritional stress. As Wolbachia are able to infect host populations it will be used in the near future to control insects that transmit disease or are commercial pests. Understanding how Wolbachia interacts with its host, as a parasite or beneficial, will improve its application in the field. Knowing how benefits are provided to the host will improve human health in the future. As Wolbachia are needed for filarial nematode development, disrupting the beneficial mechanisms identified in this study will improve filariasis control programsRead moreRead less
Cell-free immune reactions and suppression. Insects pests and insect vectors of diseases are managed by toxic substances, but insects have a cunning ability to persist. How pesticide-tolerant insect pests recognise and inactivate chemical and biological toxins is poorly understood. While vertebrates with a closed circulatory system use coagulation reactions mainly for wound-healing, invertebrates employ cell-free aggregation reactions for the sequestration and inactivation of potentially damagin ....Cell-free immune reactions and suppression. Insects pests and insect vectors of diseases are managed by toxic substances, but insects have a cunning ability to persist. How pesticide-tolerant insect pests recognise and inactivate chemical and biological toxins is poorly understood. While vertebrates with a closed circulatory system use coagulation reactions mainly for wound-healing, invertebrates employ cell-free aggregation reactions for the sequestration and inactivation of potentially damaging objects and substances. We use insect plasma to dissect recognition and inactivation of damaging objects and substances with the aim to understand tolerance and its inhibition to design novel strategies in delaying tolerance to pesticides in insect pests.Read moreRead less
Life hanging on a thread; disrupting the structure and function of lepidopteran silking behaviour. The production and use of silk by many invertebrates is vital for their survival. We develop a means of visualising and understanding the role of silking behaviour as a survival mechanism for Helicoverpa larvae (a key world-wide pest) with a view to disrupting silking. This novel project allows us to understand not just where larvae go but what they do along the way. The project outcomes will have ....Life hanging on a thread; disrupting the structure and function of lepidopteran silking behaviour. The production and use of silk by many invertebrates is vital for their survival. We develop a means of visualising and understanding the role of silking behaviour as a survival mechanism for Helicoverpa larvae (a key world-wide pest) with a view to disrupting silking. This novel project allows us to understand not just where larvae go but what they do along the way. The project outcomes will have wide application to the study and control of lepidopteran pests in natural and agricultural ecosystems and is of interest to applied and fundamental projects associated with insect pest control, behaviour and ecology.Read moreRead less
Viral and host RNA methylation in mosquitoes. Mosquitoes transmit a variety of viruses to humans and animals through blood feeding. This project aims to investigate one of the most common modifications of RNA molecules, known as N6-methyladenosine (m6A), in an important mosquito vector, Aedes aegypti, and its alterations upon infection with pathogenic as well as mosquito-specific viruses. In addition, m6A modification of viral genomic RNA and its importance in virus replication will be investiga ....Viral and host RNA methylation in mosquitoes. Mosquitoes transmit a variety of viruses to humans and animals through blood feeding. This project aims to investigate one of the most common modifications of RNA molecules, known as N6-methyladenosine (m6A), in an important mosquito vector, Aedes aegypti, and its alterations upon infection with pathogenic as well as mosquito-specific viruses. In addition, m6A modification of viral genomic RNA and its importance in virus replication will be investigated. Expected outcomes of this project include fundamental understanding of RNA methylation in mosquitoes and their role in mosquito biology and virus replication.Read moreRead less