Understanding, controlling and improving the flavour of almond kernels. Almond kernels may be sweet, semi-bitter or bitter, with the first two categories marketed as fresh nuts, while the latter are used in processed products such as marzipan. Semi-bitter kernels have a more interesting flavour than sweet kernels and we require tools to breed for this character. The bitter flavour is imparted by amygdalin via the cyanogenic pathway. This project will characterise the genetic control of sweet, ....Understanding, controlling and improving the flavour of almond kernels. Almond kernels may be sweet, semi-bitter or bitter, with the first two categories marketed as fresh nuts, while the latter are used in processed products such as marzipan. Semi-bitter kernels have a more interesting flavour than sweet kernels and we require tools to breed for this character. The bitter flavour is imparted by amygdalin via the cyanogenic pathway. This project will characterise the genetic control of sweet, semi-bitter and bitter flavour, amygdalin accumulation in developing kernels, and key enzymes in the cyanogenic pathway. Almond populations segregating for these traits will be used and the data will be integrated into the Australian almond meiotic map.Read moreRead less
Microsatellite marker development for almond breeding. Australian almond growers produce 8,000 tonnes of kernel annually, and the long term aim is to develop strong export markets. The industry funds a breeding program at Adelaide University, comprising hybridisation and selection. Through our current ARC SPIRT grant we augment this with a program in molecular techniques. Research is well-advanced in the development of a genetic map for almond based on both molecular and agronomic characters. ....Microsatellite marker development for almond breeding. Australian almond growers produce 8,000 tonnes of kernel annually, and the long term aim is to develop strong export markets. The industry funds a breeding program at Adelaide University, comprising hybridisation and selection. Through our current ARC SPIRT grant we augment this with a program in molecular techniques. Research is well-advanced in the development of a genetic map for almond based on both molecular and agronomic characters. This IREX grant will facilitate integration of our map with the International Prunus mapping program, based at Cabrils, Spain. It will also facilitate augmentation of the map with jointly-developed microsatellite markers.Read moreRead less
Investigation of almond transformation for self-fertility and virus resistance. This project addresses the long-term goal of the Australian almond industry to produce cultivars that are self-fertile and resistant to Prunus necrotic ringspot and prune dwarf viruses. Both self-sterility and virus infection can result in unreliable and often low yields. Research will address the control of gene expression relating to self-sterility and the introduction of virus resistance, via sequencing of self- ....Investigation of almond transformation for self-fertility and virus resistance. This project addresses the long-term goal of the Australian almond industry to produce cultivars that are self-fertile and resistant to Prunus necrotic ringspot and prune dwarf viruses. Both self-sterility and virus infection can result in unreliable and often low yields. Research will address the control of gene expression relating to self-sterility and the introduction of virus resistance, via sequencing of self-fertility and self-sterility genes, construct development and improved regeneration of transformed almond in vitro without the use of antibiotics.Read moreRead less
Improved olive productivity and the use of molecular markers. Sales of olive oil on international markets are growing at 15% per year, and the Australian industry is expanding rapidly to win some of this trade. Varietal identity is a major problem, which has production implications and this project will verify trueness-to type via genetic fingerprinting. Many olive cultivars require cross pollination for optimal production, and the research will identify compatible cultivars for the major variet ....Improved olive productivity and the use of molecular markers. Sales of olive oil on international markets are growing at 15% per year, and the Australian industry is expanding rapidly to win some of this trade. Varietal identity is a major problem, which has production implications and this project will verify trueness-to type via genetic fingerprinting. Many olive cultivars require cross pollination for optimal production, and the research will identify compatible cultivars for the major varieties. Molecular markers will be developed to accelerate the selection of improved varieties with self-fertility, high oleic acid, low linolenic acid and tolerance to Peacock spot disease.Read moreRead less
Integrating a physical and functional genetic map of Prunus dulcis. Genome wide physical mapping is the centrepiece of current genomics research in virtually all plant and animal species. The proposal seeks to champion the development of Prunus dulcis (Rosaceae) as a model perennial species towards parity with other plant model systems for gene discovery and validation. The Rosaceae represents a rich repository of genes of relevance to perenniality, adaptation, sustainable agriculture, health a ....Integrating a physical and functional genetic map of Prunus dulcis. Genome wide physical mapping is the centrepiece of current genomics research in virtually all plant and animal species. The proposal seeks to champion the development of Prunus dulcis (Rosaceae) as a model perennial species towards parity with other plant model systems for gene discovery and validation. The Rosaceae represents a rich repository of genes of relevance to perenniality, adaptation, sustainable agriculture, health and nutrition and the bioindustries. Ultimately, comparative genomics across the family will advance molecular eco-genetics via dissection of traits determining adaptive response. Access to user-friendly molecular markers will also bring greater precision to breeding programmes. Read moreRead less
Controlling accumulation of elements in the shoots of higher plants by manipulating processes in specific cell types in the roots. This project will provide novel, fundamental understanding of the processes controlling accumulation of elements in the shoots of plants. As such, it will impact on our understanding of processes relevant to stress tolerance, plant nutrition, human nutrition and the removal of toxic metals from soils by plants. These are all areas of great importance to Australian ag ....Controlling accumulation of elements in the shoots of higher plants by manipulating processes in specific cell types in the roots. This project will provide novel, fundamental understanding of the processes controlling accumulation of elements in the shoots of plants. As such, it will impact on our understanding of processes relevant to stress tolerance, plant nutrition, human nutrition and the removal of toxic metals from soils by plants. These are all areas of great importance to Australian agriculture, environmental sustainability and human health. The increased understanding arising from this project will underpin future work to increase agricultural productivity and the quality of life for all in the Australian and international communities.Read moreRead less
Development of advanced screening protocols for the identification of genes involved in nutrient sensing and nutrient efficiency in plants. Sustainable plant production in Australia builds on the concept of stable crop yield and high crop quality at low pesticide and fertilizer input. This requires a more efficient use of the plant's own mechanisms to efficiently explore nutrient patches in soils that are usually heterogeneously distributed. The identification of nutrient sensors in plants will ....Development of advanced screening protocols for the identification of genes involved in nutrient sensing and nutrient efficiency in plants. Sustainable plant production in Australia builds on the concept of stable crop yield and high crop quality at low pesticide and fertilizer input. This requires a more efficient use of the plant's own mechanisms to efficiently explore nutrient patches in soils that are usually heterogeneously distributed. The identification of nutrient sensors in plants will not only allow a deeper understanding of how plants manage to overcome nutrient-poor growth periods but also open new possibilities for enhancing nutrient efficiency in crop plants. The increased understanding arising from this project will underpin future work to increase agricultural productivity and the quality of life for all in the Australian and international communities.Read moreRead less
Salinity tolerance and long-distance transport in cereals. The aim of this program is to alter shoot accumulation of solutes in cereals by exploiting novel transgenic technology to manipulate processes in specific cell types in the roots. The primary objective is the generation of cereals which have increased tolerance of saline soils. This is clearly of much agricultural significance in Australia. More general outcomes include the generation of plants with altered concentrations of a range of n ....Salinity tolerance and long-distance transport in cereals. The aim of this program is to alter shoot accumulation of solutes in cereals by exploiting novel transgenic technology to manipulate processes in specific cell types in the roots. The primary objective is the generation of cereals which have increased tolerance of saline soils. This is clearly of much agricultural significance in Australia. More general outcomes include the generation of plants with altered concentrations of a range of nutrients in both leaves and grain. This will be of wide agricultural and nutritional benefit, as well as providing an understanding of principles underlying the long-distance co-ordination of processes in plants.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453684
Funder
Australian Research Council
Funding Amount
$301,921.00
Summary
Joint facility for genome analysis. This project will establish a joint facility for genome analysis supported by the Universities of Adelaide and South Australia, the Australian Centre for Plant Functional Genomics and the Australian Wine Research Institute. The facility will purchase novel equipment for high-throughput gene selection and screening, advanced DNA and protein imaging and a dedicated reconfigurable computing platform for advanced bioinformatic analysis. The equipment has been ta ....Joint facility for genome analysis. This project will establish a joint facility for genome analysis supported by the Universities of Adelaide and South Australia, the Australian Centre for Plant Functional Genomics and the Australian Wine Research Institute. The facility will purchase novel equipment for high-throughput gene selection and screening, advanced DNA and protein imaging and a dedicated reconfigurable computing platform for advanced bioinformatic analysis. The equipment has been targeted to overcome technical barriers that limit the rapid adoption of genome discovery projects in South Australia. This facility will result in new plant gene discovery and improved understanding of fundamental plant processes.Read moreRead less
How common and what is the significance of cis-acting regulatory variation and genomic imprinting in plants? Plant based processes provide truly sustainable solutions to many of the challenges facing Australia. The proposed research will help elucidate how plants regulate variation in gene expression rather than changes in the structure of encoded proteins. This is an area of gene expression, that has not been approached before, that explains the plant's ability to respond to external stimuli. ....How common and what is the significance of cis-acting regulatory variation and genomic imprinting in plants? Plant based processes provide truly sustainable solutions to many of the challenges facing Australia. The proposed research will help elucidate how plants regulate variation in gene expression rather than changes in the structure of encoded proteins. This is an area of gene expression, that has not been approached before, that explains the plant's ability to respond to external stimuli. Variation between plant species in the number of genes does not fully explain the differences between them. This information will come not from seqenceing genomes but from investigation of regulatory elements. Read moreRead less