The role of phosphoinositides in endosomal maturation dynamics. This project aims to investigate the regulation of an intracellular compartment within a cell called endosomes, which plays critical roles in cellular homeostasis, signalling and pathogen entry. New knowledge is expected to be generated in understanding endosome maturation and the signalling events that drive this process using a unique, multidisciplinary approach combining state of the art imaging techniques and high throughput pro ....The role of phosphoinositides in endosomal maturation dynamics. This project aims to investigate the regulation of an intracellular compartment within a cell called endosomes, which plays critical roles in cellular homeostasis, signalling and pathogen entry. New knowledge is expected to be generated in understanding endosome maturation and the signalling events that drive this process using a unique, multidisciplinary approach combining state of the art imaging techniques and high throughput protein analysis. The anticipated outcomes will be to define the molecular steps that govern the membrane-bound machinery on endosomes that directs endosomal maturation. This should provide significant benefits in delineating a process that is linked to almost all aspects of cell life.Read moreRead less
Manipulation of mitochondrial function by Legionella pneumophila. . The intracellular bacterial pathogen Legionella pneumophila co-evolved with eukaryotic hosts and has developed sophisticated mechanisms to manipulate human cell function – mitochondria in particular – by secreting >300 effector proteins through a specialised Type-IV system into the host cell. This research aims to understand the function of effector proteins targeted to mitochondria; delivering important new knowledge in host-pa ....Manipulation of mitochondrial function by Legionella pneumophila. . The intracellular bacterial pathogen Legionella pneumophila co-evolved with eukaryotic hosts and has developed sophisticated mechanisms to manipulate human cell function – mitochondria in particular – by secreting >300 effector proteins through a specialised Type-IV system into the host cell. This research aims to understand the function of effector proteins targeted to mitochondria; delivering important new knowledge in host-pathogen and mitochondrial biology and advanced cell biology tools. With most of the effector proteins yet to be characterised, benefits from the project will be to reveal specifically how these target mitochondria, and more broadly, how bacterial pathogens manipulate organelles for their survival.Read moreRead less
Tracking DNA repair dynamics in the nuclear landscape of a living cell. This project aims to track DNA repair factor recruitment in the nuclear landscape of a living cell and quantify the role of nucleus architecture in maintenance of genome integrity. By coupling advanced fluorescence microscopy with a novel DNA double strand break inducible cell system, this project expects to uncover how the nucleus spatially coordinates DNA damage detection, assessment and repair in real time. This research ....Tracking DNA repair dynamics in the nuclear landscape of a living cell. This project aims to track DNA repair factor recruitment in the nuclear landscape of a living cell and quantify the role of nucleus architecture in maintenance of genome integrity. By coupling advanced fluorescence microscopy with a novel DNA double strand break inducible cell system, this project expects to uncover how the nucleus spatially coordinates DNA damage detection, assessment and repair in real time. This research is important because DNA damage threatens organism survival and this project has the potential to define how this genomic threat is resolved at the single molecule level. The benefit of this research is a fundamental insight into DNA repair biology and development of imaging technology to quantify genome function.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE230100700
Funder
Australian Research Council
Funding Amount
$429,449.00
Summary
A novel bacterial secretion system for applications in nanobiotechnology. This project aims to characterise a new molecular machine, called the S-Pump. Molecular machines drive the complex biology in all cells and are an exciting area of translational research, with broad potential for industrial applications. This project expects to provide fundamental insights into how bacterial S-Pumps contribute to antimicrobial resistance and enhancing food production. Expected outcomes include new tools fo ....A novel bacterial secretion system for applications in nanobiotechnology. This project aims to characterise a new molecular machine, called the S-Pump. Molecular machines drive the complex biology in all cells and are an exciting area of translational research, with broad potential for industrial applications. This project expects to provide fundamental insights into how bacterial S-Pumps contribute to antimicrobial resistance and enhancing food production. Expected outcomes include new tools for molecular machine discovery and identification of ways to adapt molecular machines for biotechnological applications. This work should enhance Australia-UK ties through collaboration, provide benefits toward nanobiotechnology and economic benefits through more efficient food production.Read moreRead less
Mapping the integration of T cell fate control across time and space. This project aims to apply new methods to determine how coordination of signalling complexes impacts upon the fate of cells of the adaptive immune system. It expects to determine how the context of signallng orchestrates cell fates such as differentiation, death and proliferation. The project is expected to yield an experimental and analytical platform for further investigations into a broad range of biological questions, and ....Mapping the integration of T cell fate control across time and space. This project aims to apply new methods to determine how coordination of signalling complexes impacts upon the fate of cells of the adaptive immune system. It expects to determine how the context of signallng orchestrates cell fates such as differentiation, death and proliferation. The project is expected to yield an experimental and analytical platform for further investigations into a broad range of biological questions, and to provide new knowledge of this fundamental problem. This platform should support further work that ultimately provides new models for tissue and immune cell regeneration, and new manufacturing platforms for therapies for humans and livestock, among other benefits.Read moreRead less
Co-designing Innovations in Digital Storytelling with Older Adults. This project aims to investigate how emerging technologies can be leveraged to provide innovative ways for older adults to create and share their life stories to foster social wellbeing. Later life can be a time of considerable change, leaving people feeling disconnected from the people, places, and life events that are important to them. Autobiographical storytelling can help create links with one's past, but little is known ab ....Co-designing Innovations in Digital Storytelling with Older Adults. This project aims to investigate how emerging technologies can be leveraged to provide innovative ways for older adults to create and share their life stories to foster social wellbeing. Later life can be a time of considerable change, leaving people feeling disconnected from the people, places, and life events that are important to them. Autobiographical storytelling can help create links with one's past, but little is known about how technologies such as digital games and virtual reality can be used to enable older adults to share stories about their lives in a way that supports ongoing social interactions. This project is expected to co-design new forms of digital storytelling to improve social wellbeing of older adults. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE210100046
Funder
Australian Research Council
Funding Amount
$289,381.00
Summary
A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolut ....A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolution. The benefit of this technology is that it will enable scientists in Australia to image, for the first time, the biophysical mechanism by which a protein navigates intracellular architecture to regulate a complex biological function at the single molecule level.Read moreRead less
Designing distanced intergenerational interaction with tangible technology. Older people and their young relatives/grandchildren who are geographically distanced cannot currently experience closeness in tangible ways, which are the natural ways they would play and build relationships in “real” life. Enabling this connection would have positive impacts for both groups, and two types of technologies – Mixed Reality and Tangibles - can be explored to allow us to understand how to do this. We will d ....Designing distanced intergenerational interaction with tangible technology. Older people and their young relatives/grandchildren who are geographically distanced cannot currently experience closeness in tangible ways, which are the natural ways they would play and build relationships in “real” life. Enabling this connection would have positive impacts for both groups, and two types of technologies – Mixed Reality and Tangibles - can be explored to allow us to understand how to do this. We will develop approaches to distanced tangible intergenerational interaction which are designed specifically to increase intergenerational closeness and to be innovative and subtle so that they fit seamlessly into the lives of older people and young children.Read moreRead less
(Re)Designing Digital Justice. This project aims to address the challenge of (re)designing novel online court systems by introducing a human-centred design process to the legal process. This project will generate fundamental new knowledge in respect of how to effectively design an inclusive justice system, bridging the gap between the legal system and human-computer interaction. Expected outcomes include how to use technology to implement a more just, efficient, and fair legal system, which is a ....(Re)Designing Digital Justice. This project aims to address the challenge of (re)designing novel online court systems by introducing a human-centred design process to the legal process. This project will generate fundamental new knowledge in respect of how to effectively design an inclusive justice system, bridging the gap between the legal system and human-computer interaction. Expected outcomes include how to use technology to implement a more just, efficient, and fair legal system, which is accessible to all Australians. This should provide significant benefits for both Australian society and the legal system.Read moreRead less