Linkage Infrastructure, Equipment And Facilities - Grant ID: LE180100157
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Confocal and single molecule microscopes for systems microscopy. This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information th ....Confocal and single molecule microscopes for systems microscopy. This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information that is not easily obtainable with other approaches. The project will enable Australian researchers to image and analyse the full complexity of biological systems, potentially transforming cell biology, drug development and understanding the molecular basis of disease. It will also demonstrate how the capacity of microscopy facilities can be enhanced and bias in imaging data reduced by automating data acquisition and mining of image-based data.Read moreRead less
New targets for antiviral therapies. The ability of dangerous viruses to cause lethal disease depends on their capacity to evade the immune system of infected hosts. This project will uncover at the molecular level the strategies used by viruses to disable immune responses; this will identify new ways to treat incurable diseases, by disabling the virus' defences against the immune system.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE210100046
Funder
Australian Research Council
Funding Amount
$289,381.00
Summary
A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolut ....A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolution. The benefit of this technology is that it will enable scientists in Australia to image, for the first time, the biophysical mechanism by which a protein navigates intracellular architecture to regulate a complex biological function at the single molecule level.Read moreRead less
Composition, assembly and functions of the pellicle of apicomplexan parasites: a structure pivotal to disease transmission and progression. Apicomplexan parasites are successful agents of disease (e.g. malaria) due to their superb ability to quickly invade host cells and generate many more parasites. This project will study the dedicated structures beneath the parasite cell covering that are responsible for these abilities to help refine strategies for combating apicomplexan diseases.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100078
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Multiphoton confocal microscope. Recent developments in light microscopy have revolutionised modern molecular and cellular biology. Dramatic improvements in microscope hardware and software and in the range of fluorescent markers used to tag selected cellular components now provide new and exciting opportunities to localise and determine the function of ions and molecules not only in preserved samples but also, most excitingly, in living cells. The proposed multiphoton confocal microscope will ....Multiphoton confocal microscope. Recent developments in light microscopy have revolutionised modern molecular and cellular biology. Dramatic improvements in microscope hardware and software and in the range of fluorescent markers used to tag selected cellular components now provide new and exciting opportunities to localise and determine the function of ions and molecules not only in preserved samples but also, most excitingly, in living cells. The proposed multiphoton confocal microscope will allow researchers in Canberra to obtain high quality images of static and moving components in living cells and tissues and will facilitate the discovery of new knowledge that contributes to our understanding and control of development and disease in both plants and animals.Read moreRead less
Protein trafficking pathways in fungal rust pathogens of plants. This project aims to investigate protein trafficking pathways in fungal rusts of plants. These are devastating diseases that cause major losses in agricultural crops including wheat. Little is known of how these fungi form long and intimate relationships with plants to extract their nutrients. Using both model and agriculturally important fungi, the project will try to identify proteins that are transferred within the fungus and to ....Protein trafficking pathways in fungal rust pathogens of plants. This project aims to investigate protein trafficking pathways in fungal rusts of plants. These are devastating diseases that cause major losses in agricultural crops including wheat. Little is known of how these fungi form long and intimate relationships with plants to extract their nutrients. Using both model and agriculturally important fungi, the project will try to identify proteins that are transferred within the fungus and to the plant host to modify the infection. It will also identify fungal proteins needed to deliver trafficked proteins to plants. These discoveries ultimately may be translated into control strategies for these costly diseases.Read moreRead less