FUNCTIONAL GENOMICS OF PLANT MITOCHONDRIA: THEIR ROLE IN DEVELOPMENT, OXIDATIVE STRESS AND PLANT DEFENSE. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in th ....FUNCTIONAL GENOMICS OF PLANT MITOCHONDRIA: THEIR ROLE IN DEVELOPMENT, OXIDATIVE STRESS AND PLANT DEFENSE. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen attack, and in production of reactive oxygen molecules. This proposal seeks to investigate how mitochondria are involved in these processes, using the latest plant genome information. Potential outcomes include crops better able to cope with environmental stress.Read moreRead less
Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across sout ....Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across southern Australia. Benefits include prevention of severe losses in canola from downy mildew, and more viable and sustainable production with less reliance upon fungicides. This research addresses the National Research Priority 'An Environmentally Sustainable Australia' and the Priority Goal of 'Transforming existing industries', and will particularly benefit southern Australian rural communities.Read moreRead less
Race status, resistance mechanisms, and new sources of resistance to Phytophthora clandestina, a major threat to subterranean clover production. Devastating outbreaks of disease caused by Phytophthora clandestina (Phytophthora root rot) since the late 1970s demonstrated the capacity of this disease to impact severely on clover pasture production across southern Australia, particularly as new races of Phytophthora have rapidly emerged to overcome the resistance of all commercial cultivars. The p ....Race status, resistance mechanisms, and new sources of resistance to Phytophthora clandestina, a major threat to subterranean clover production. Devastating outbreaks of disease caused by Phytophthora clandestina (Phytophthora root rot) since the late 1970s demonstrated the capacity of this disease to impact severely on clover pasture production across southern Australia, particularly as new races of Phytophthora have rapidly emerged to overcome the resistance of all commercial cultivars. The proposed research seeks to delineate new races of the pathogen, to identify the histological and biochemical mechanisms by which resistance to Phytophthora root rot is expressed, and to identify new sources of host resistance. This proposed research will enable breeders, for the first time, to incorporate multiple types of resistance and against different races into new host varieties.Read moreRead less
Characterizing the regulators of mitochondrial biogenesis in Arabidopsis thaliana. The overall aim of this project is to identify and characterise the underlying regulatory factors that control mitochondrial mass and number in plants. The project will exploit a regulatory mechanism that links the mitochondrial import machinery and the respiratory chain. Utilising both forward and reverse genetic approaches, the abundances of protein import translocases will be altered and the changes to mitochon ....Characterizing the regulators of mitochondrial biogenesis in Arabidopsis thaliana. The overall aim of this project is to identify and characterise the underlying regulatory factors that control mitochondrial mass and number in plants. The project will exploit a regulatory mechanism that links the mitochondrial import machinery and the respiratory chain. Utilising both forward and reverse genetic approaches, the abundances of protein import translocases will be altered and the changes to mitochondrial biogenesis will be investigated. This will identify regulatory factors, which can be manipulated and used to alter mitochondrial number and activity.Read moreRead less
Defining factors that influence protein turnover in plants. This project aims to discover how the functions of different proteins change as they age, and to define factors that dictate protein stability inside plants. This project will change protein turnover rates in plants by altering a regulator of this process to assess the role of this regulator in different plant developmental transitions. Expected outcomes include showing how protein abundance can be altered in plants for our future agric ....Defining factors that influence protein turnover in plants. This project aims to discover how the functions of different proteins change as they age, and to define factors that dictate protein stability inside plants. This project will change protein turnover rates in plants by altering a regulator of this process to assess the role of this regulator in different plant developmental transitions. Expected outcomes include showing how protein abundance can be altered in plants for our future agricultural and biotechnology needs. This will provide significant benefits, such as discovering processes inside plant cells that maintain the quality of different kinds of proteins and propose how different kinds of proteins can be stabilised for plant biotechnology applications.Read moreRead less
Discovery of the molecular mode of action of karrikins in plants. Karrikins are a newly-discovered family of naturally-occurring plant growth regulators that stimulate seed germination and seedling vigour. They were discovered in smoke and while they are centrally important in fire ecology they have far wider significance since species from non-fire-prone regions also respond to karrikins. Our research will discover how karrikins work at the molecular level in plant cells. Our discoveries will b ....Discovery of the molecular mode of action of karrikins in plants. Karrikins are a newly-discovered family of naturally-occurring plant growth regulators that stimulate seed germination and seedling vigour. They were discovered in smoke and while they are centrally important in fire ecology they have far wider significance since species from non-fire-prone regions also respond to karrikins. Our research will discover how karrikins work at the molecular level in plant cells. Our discoveries will be applied to improve growth of crop plants, to stimulate germination of weeds so that they can be eradicated, and in restoration ecology to revegetate degraded land such as minesites. Australia's world-leading position in this new important research area will be enhanced.Read moreRead less
Dual-targeting of proteins and its role in coordinating organelle functions in plants. Innovative agricultural solutions in Australia's future will be built on understanding and manipulating the expression of groups of genes to influence whole plant phenotypes providing more robust plants and high value plant products. Plant energy organelles are central components in plant metabolism, their coordination by processes such as dual-targeting has potential to modify germination characteristics, ear ....Dual-targeting of proteins and its role in coordinating organelle functions in plants. Innovative agricultural solutions in Australia's future will be built on understanding and manipulating the expression of groups of genes to influence whole plant phenotypes providing more robust plants and high value plant products. Plant energy organelles are central components in plant metabolism, their coordination by processes such as dual-targeting has potential to modify germination characteristics, early seedling vigour, and stress tolerance. Studying energy organelles could generate valuable intellectual property to be applied within Australia's large plant-based industries and at the same time provide a rich intellectual environment for the training of research students and postdoctoral researchers.Read moreRead less
The role of changes to the proteome in the signalling of stress response in plant mitochondria. Innovative agricultural solutions in Australia's harsh climate will be built on manipulating the expression of groups of genes and understanding how the proteins they encode operate to influence whole plant phenotypes under stress to provide more robust plants and improved plant products. Mitochondria are central components in plant metabolism. Stabilizing their function during stress has the potentia ....The role of changes to the proteome in the signalling of stress response in plant mitochondria. Innovative agricultural solutions in Australia's harsh climate will be built on manipulating the expression of groups of genes and understanding how the proteins they encode operate to influence whole plant phenotypes under stress to provide more robust plants and improved plant products. Mitochondria are central components in plant metabolism. Stabilizing their function during stress has the potential to modify germination characteristics, early seedling vigour, and stress tolerance. Studying plant mitochondria supports the generation of intellectual property to be applied within Australia's plant-based industries and at the same time provide a rich intellectual environment for the training of students and researchers.Read moreRead less
The targeting of macromolecules to alter mitochondrial function. Mitochondria are essential organelles involved in energy production and specific metabolic pathways in plant cells that require the import of cytosolic transfer RNA (tRNA) to function. To date our knowledge on the mechanisms of tRNA import is limited. This project seeks to characterise putative receptors and mechanisms with the purpose of exploiting these insights to allow for the manipulation and modification of macromolecule targ ....The targeting of macromolecules to alter mitochondrial function. Mitochondria are essential organelles involved in energy production and specific metabolic pathways in plant cells that require the import of cytosolic transfer RNA (tRNA) to function. To date our knowledge on the mechanisms of tRNA import is limited. This project seeks to characterise putative receptors and mechanisms with the purpose of exploiting these insights to allow for the manipulation and modification of macromolecule targeting to mitochondria. The ability to modify or alter mitochondrial biogenesis and activity may allow for new approaches to be undertaken to increase plant growth, productivity and resistance to stress.Read moreRead less
Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We ....Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We will assign
function to each protein and investigate the importance in regulating organelle biogenesis. This will allow us to
modulate plant energy production for optimal growth and to withstand abiotic stress, all of which have
agriculturally beneficial consequences. Read moreRead less