Tracking DNA repair dynamics in the nuclear landscape of a living cell. This project aims to track DNA repair factor recruitment in the nuclear landscape of a living cell and quantify the role of nucleus architecture in maintenance of genome integrity. By coupling advanced fluorescence microscopy with a novel DNA double strand break inducible cell system, this project expects to uncover how the nucleus spatially coordinates DNA damage detection, assessment and repair in real time. This research ....Tracking DNA repair dynamics in the nuclear landscape of a living cell. This project aims to track DNA repair factor recruitment in the nuclear landscape of a living cell and quantify the role of nucleus architecture in maintenance of genome integrity. By coupling advanced fluorescence microscopy with a novel DNA double strand break inducible cell system, this project expects to uncover how the nucleus spatially coordinates DNA damage detection, assessment and repair in real time. This research is important because DNA damage threatens organism survival and this project has the potential to define how this genomic threat is resolved at the single molecule level. The benefit of this research is a fundamental insight into DNA repair biology and development of imaging technology to quantify genome function.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE210100046
Funder
Australian Research Council
Funding Amount
$289,381.00
Summary
A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolut ....A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolution. The benefit of this technology is that it will enable scientists in Australia to image, for the first time, the biophysical mechanism by which a protein navigates intracellular architecture to regulate a complex biological function at the single molecule level.Read moreRead less
Nuclear architecture in a living cell facilitates navigation of the genome. This project aims to investigate the role of nuclear architecture in regulating genome function by development of a new microscopy method to quantify the diffusive route of fluorescent proteins in live cells. The anticipated outcomes of this project include an insight into how chromatin dynamics facilitate DNA target search and an analytical tool for cell biologists to probe how genomes work in their natural environment ....Nuclear architecture in a living cell facilitates navigation of the genome. This project aims to investigate the role of nuclear architecture in regulating genome function by development of a new microscopy method to quantify the diffusive route of fluorescent proteins in live cells. The anticipated outcomes of this project include an insight into how chromatin dynamics facilitate DNA target search and an analytical tool for cell biologists to probe how genomes work in their natural environment (the cell nucleus).Read moreRead less
Structural domains of beta-tubulin and their role in microtubule dynamics and transport. This study aims to obtain a fundamental understanding of how the structural domains of the cytoskeletal protein beta-tubulin are involved in microtubule structures during cell division and vesicular transport. Using gene-editing technology and coupling this with cell biological approaches and high-resolution cell imaging will enable detailed analysis of the role of beta-tubulin domains in these important cel ....Structural domains of beta-tubulin and their role in microtubule dynamics and transport. This study aims to obtain a fundamental understanding of how the structural domains of the cytoskeletal protein beta-tubulin are involved in microtubule structures during cell division and vesicular transport. Using gene-editing technology and coupling this with cell biological approaches and high-resolution cell imaging will enable detailed analysis of the role of beta-tubulin domains in these important cellular processes. The outcomes will include fundamental new knowledge in cell biology and lead to the development of unique biological models that can be used to understand disease.Read moreRead less
Novel tools and nanotechnology to navigate intracellular trafficking. This project aims to investigate how material accesses different compartments inside cells, also known as trafficking. Using immunology, cell biology and nanotechnology, the project will manipulate intracellular trafficking to achieve specific cellular functions. Outcomes will also form the basis of intellectual property development for new products by Australian biotechnology companies. These products will improve veterinary ....Novel tools and nanotechnology to navigate intracellular trafficking. This project aims to investigate how material accesses different compartments inside cells, also known as trafficking. Using immunology, cell biology and nanotechnology, the project will manipulate intracellular trafficking to achieve specific cellular functions. Outcomes will also form the basis of intellectual property development for new products by Australian biotechnology companies. These products will improve veterinary and human health services, leading to increased productivity.Read moreRead less
Gut Absorption of Constrained Peptides for Local and Systemic Targeting. Aims: This project aims to investigate how peptides are absorbed across the intestinal wall and distributed to organs and fluids in a rodent model by combining bio-analysis and pharmacokinetics with high-resolution microscopy and imaging.
Significance: This project expects to generate the most comprehensive survey to date of the pathways and mechanisms of peptide absorption, biodistribution and immune cell targeting, by ....Gut Absorption of Constrained Peptides for Local and Systemic Targeting. Aims: This project aims to investigate how peptides are absorbed across the intestinal wall and distributed to organs and fluids in a rodent model by combining bio-analysis and pharmacokinetics with high-resolution microscopy and imaging.
Significance: This project expects to generate the most comprehensive survey to date of the pathways and mechanisms of peptide absorption, biodistribution and immune cell targeting, by implementing innovative approaches.
Expected Outcomes: Expected outcomes include significant new knowledge and a new multi-disciplinary platform for measuring peptide absorption.
Benefits: This should provide significant benefits by informing the future design of peptides for supplements, therapeutics and carriers. Read moreRead less
The molecular blue-print for a mitochondrial nanomachine. The objective of the project is to develop a comprehensive understanding of the architecture of a biological nanomachine through broad-reaching investigation of the molecular contacts that enable the component parts to work together. The project plans to take the foundation knowledge of each of the component parts and build a conceptual framework of engineering principles to understand how the nanomachine is assembled, using a breakthroug ....The molecular blue-print for a mitochondrial nanomachine. The objective of the project is to develop a comprehensive understanding of the architecture of a biological nanomachine through broad-reaching investigation of the molecular contacts that enable the component parts to work together. The project plans to take the foundation knowledge of each of the component parts and build a conceptual framework of engineering principles to understand how the nanomachine is assembled, using a breakthrough technology to address the precise architecture of the component parts within the nanomachine.Read moreRead less
The cell biology of the albumin-FcRn receptor recycling system. The aim of this project is to define the cell biology of the albumin-FcRn (neonatal Fc receptor) recycling system. FcRn is a recycling membrane receptor that selectively protects serum proteins from intracellular degradation and prolongs their half-life. We will identify the key cell types involved in this recycling pathway, identify intracellular sites of ligand and FcRn interaction, assess the contribution of the haematopoietic sy ....The cell biology of the albumin-FcRn receptor recycling system. The aim of this project is to define the cell biology of the albumin-FcRn (neonatal Fc receptor) recycling system. FcRn is a recycling membrane receptor that selectively protects serum proteins from intracellular degradation and prolongs their half-life. We will identify the key cell types involved in this recycling pathway, identify intracellular sites of ligand and FcRn interaction, assess the contribution of the haematopoietic system and determine ligand half-life in mice. Findings generated will reveal the basic biology of an important physiological receptor, and enable the exploitation of FcRn-receptor interactions for design of recombinant albumin fusion-based therapies.Read moreRead less