Enhanced biocontrol options for the Australian sugar industry: a proteomic approach. Sugarcane is the second largest field crop grown in Australia and raw sugar is the third largest export product. Canegrubs are the major insect pest affecting production of sugarcane in Australia, currently costing the industry more than $12 million annually in insecticides and lost production. Biological control of insect pests provides an environmentally sound option of reducing these losses. Here we propos ....Enhanced biocontrol options for the Australian sugar industry: a proteomic approach. Sugarcane is the second largest field crop grown in Australia and raw sugar is the third largest export product. Canegrubs are the major insect pest affecting production of sugarcane in Australia, currently costing the industry more than $12 million annually in insecticides and lost production. Biological control of insect pests provides an environmentally sound option of reducing these losses. Here we propose to use proteomics to identify the key pathogenicity determinants in the biological control fungus Metarhizium during infection of canegrubs. The genes and proteins identified in this project will contribute to developing enhanced biocontrol options for Australian sugar industry.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0454052
Funder
Australian Research Council
Funding Amount
$733,595.00
Summary
Tandem Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometer and Robots for High Throughput Proteomics Analysis. This proposal seeks to establish the capacity to perform high-energy tandem mass spectrometry on a high throughput basis, through purchase and coordinated operation of a Matrix-Assisted Laser Desorption/Ionisation - Time of Flight / Time of Flight - Mass Spectrometer and ancillary equipment, to enhance the proteomics expertise, infrastructure and research plans ....Tandem Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometer and Robots for High Throughput Proteomics Analysis. This proposal seeks to establish the capacity to perform high-energy tandem mass spectrometry on a high throughput basis, through purchase and coordinated operation of a Matrix-Assisted Laser Desorption/Ionisation - Time of Flight / Time of Flight - Mass Spectrometer and ancillary equipment, to enhance the proteomics expertise, infrastructure and research plans of a network of institutions from Queensland and New South Wales and their collaborators. Access to such instrumentation is critical to high level achievement in proteomics, a key platform technology for National Research Priorities relating to Frontier Technologies. No comparable instrument currently exists in Australia.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989105
Funder
Australian Research Council
Funding Amount
$495,000.00
Summary
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national i ....An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national importance. These include understanding the impact of the environment on plant and animal development, pest animal control, development of new biotechnology tools, new drugs and new methods for the detection of narcotics and explosives.Read moreRead less
O-GlcNAc-phosphorylation: a novel post-translational modification regulating vesicle recycling. We will determine a biological role for our discovery of a hybrid protein modification (both carbohydrate and phosphate) on a brain protein that is involved in nerve cell communication. If this modification is more widespread, then we will have discovered a new level of cellular regulation. This discovery is likely to have a broad benefit. It will advance the understanding of carbohydrate and phosphat ....O-GlcNAc-phosphorylation: a novel post-translational modification regulating vesicle recycling. We will determine a biological role for our discovery of a hybrid protein modification (both carbohydrate and phosphate) on a brain protein that is involved in nerve cell communication. If this modification is more widespread, then we will have discovered a new level of cellular regulation. This discovery is likely to have a broad benefit. It will advance the understanding of carbohydrate and phosphate modified proteins. For example, there may be consequences for the model of hyperphosphorylated and carbohydrate modified proteins involved in neurodegeneration. There will also be a targeted benefit. An improved understanding of the mechanism of neurotransmission will benefit in designing compounds to fight diseases of neurotransmission.Read moreRead less
How do cells regulate the synthesis and localisation of coenzyme Q? The aims of this project are to identify how cells regulate the synthesis and the distribution of coenzyme Q between different organelles, and how these processes are affected when cells experience various conditions of stress. Coenzyme Q is a fat-soluble molecule present in all cell membranes and essential for normal cell function. Despite this, relatively little is known about the systems that regulate the synthesis and cellul ....How do cells regulate the synthesis and localisation of coenzyme Q? The aims of this project are to identify how cells regulate the synthesis and the distribution of coenzyme Q between different organelles, and how these processes are affected when cells experience various conditions of stress. Coenzyme Q is a fat-soluble molecule present in all cell membranes and essential for normal cell function. Despite this, relatively little is known about the systems that regulate the synthesis and cellular location of coenzyme Q. The project plans to identify the genes and proteins required for coenzyme Q regulation of sub-cellular distribution in unstressed and stressed cells. In doing so, the project could provide a greater understanding of the ways cells maintain normal coenzyme Q levels and respond to stress.Read moreRead less