Structure and dynamics of a multiprotein-mRNA complex involved in the regulation of gene expression. RNA/protein interactions are now recognised as a major control point in the regulation of gene-expression. Proteins such as HuR and the poly(C)-binding proteins (PCBPs) act to stabilise and transport specific messenger (m)RNAs, and thus determine their translation levels. In contrast to such an important function, very little is known about these protein/mRNA interactions at an atomic level. The ....Structure and dynamics of a multiprotein-mRNA complex involved in the regulation of gene expression. RNA/protein interactions are now recognised as a major control point in the regulation of gene-expression. Proteins such as HuR and the poly(C)-binding proteins (PCBPs) act to stabilise and transport specific messenger (m)RNAs, and thus determine their translation levels. In contrast to such an important function, very little is known about these protein/mRNA interactions at an atomic level. The current study will investigate the structural and biophysical properties of a recently discovered HuR/PCBP/mRNA complex implicated in the regulation of androgen receptor expression. This information has the potential to assist in the development of drugs to reduce AR expression in prostate cancer.Read moreRead less
Unlocking self-healing bio-concrete through multiscale modelling. Self-healing bio-concrete, which uses bacteria as means to repair cracks, has the potential to revolutionise the construction industry and reduce the infrastructure repair and maintenance cost by billions of dollars annually. To unlock this, we need to understand the bacterial self-healing mechanisms for effective control of the performance. This project will develop a multiscale framework to describe the competing mechanisms betw ....Unlocking self-healing bio-concrete through multiscale modelling. Self-healing bio-concrete, which uses bacteria as means to repair cracks, has the potential to revolutionise the construction industry and reduce the infrastructure repair and maintenance cost by billions of dollars annually. To unlock this, we need to understand the bacterial self-healing mechanisms for effective control of the performance. This project will develop a multiscale framework to describe the competing mechanisms between crack widening and healing at the macro-scale, incorporated with key information of substances diffusion and bio-cementation at the meso- and micro-scales. This will enable to optimise the self-healing of bio-concrete via design–test–learn approach and enhance the durability of structures under sustained loads.Read moreRead less
Calcification of acrylic hydrogels in abiotic media: mechanism and control. Poly(2-hydroxyethyl methacrylate (PHEMA) and other acrylic hydrogels are extensively used as biomaterials, yet conclusive evidence exists that they have a propensity to calcify following implantation. This process has undesirable consequences on the functionality of various prostheses. Based on preliminary observations that PHEMA can promote the deposition of calcium minerals from media devoid of biological factors, whic ....Calcification of acrylic hydrogels in abiotic media: mechanism and control. Poly(2-hydroxyethyl methacrylate (PHEMA) and other acrylic hydrogels are extensively used as biomaterials, yet conclusive evidence exists that they have a propensity to calcify following implantation. This process has undesirable consequences on the functionality of various prostheses. Based on preliminary observations that PHEMA can promote the deposition of calcium minerals from media devoid of biological factors, which appears thus to be an inherent property of the polymer, the project aims at formulating new hypotheses to explain this phenomenon, and to confirm them experimentally. The "chelation" hypothesis will be validated by modifying the structure of polymers, and the "spontaneous precipitation" hypothesis by assessing the effect of solutes on the equilibrium water content of polymers. NMR and FTIR spectrometric techniques will be used to gain further insight into the mechanism of calcification. Methods to prevent the calcification will potentially result from these experiments, however, anticalcification agents will also be incorporated into hydrogels and their effect evaluated in calcification assays.Read moreRead less
Interaction between consolidation and lubrication of biological joints. This project aims to develop a computational model to be used in conjunction with experimental studies to understand complex lubrication systems in biological joints. Nature has equipped biological joints with a remarkable ability to achieve ultralow friction even at relatively high contact force, however the mechanisms used remain uncertain. This project intends to provide a deeper, fundamental understanding of the friction ....Interaction between consolidation and lubrication of biological joints. This project aims to develop a computational model to be used in conjunction with experimental studies to understand complex lubrication systems in biological joints. Nature has equipped biological joints with a remarkable ability to achieve ultralow friction even at relatively high contact force, however the mechanisms used remain uncertain. This project intends to provide a deeper, fundamental understanding of the friction and contact mechanisms occurring in biological joints. The project outcomes could lead to bioinspired innovation in future engineering design and advancements in materials science that have the potential to significantly benefit Australian society.Read moreRead less
Brain-skull interface: discovering the missing piece of head biomechanics. Overall objective of this project is to measure, mathematically describe and implement in software mechanical properties of brain-skull interface – a critical component of current large and sophisticated computational models of the brain and the last missing piece of brain biomechanics knowledge. This will allow increased reliability of comprehensive biomechanical models used to simulate realistic injury and surgery scena ....Brain-skull interface: discovering the missing piece of head biomechanics. Overall objective of this project is to measure, mathematically describe and implement in software mechanical properties of brain-skull interface – a critical component of current large and sophisticated computational models of the brain and the last missing piece of brain biomechanics knowledge. This will allow increased reliability of comprehensive biomechanical models used to simulate realistic injury and surgery scenarios.
The problem is significant and urgent. Every year in Australia, there are over 22,000 cases of traumatic brain injury, some of which could be prevented by better passive and active countermeasures; and over 12,000 neurosurgical procedures that surgical simulation could make more accurate and therefore safer.Read moreRead less
Neuroimage as biomechanical model: new real-time computational biomechanics of the brain. This project is to extend to medicine the success computational mechanics has enjoyed in traditional engineering. The project will create enabling modelling and computing technologies for Computer-Integrated Surgery Systems that could help to improve clinical outcomes and the efficiency of health care delivery.
Mesoporous Metal Scaffolds: Reactive Containment Vessels. The storage of hydrogen is one of the most important issues that remains to be solved before the mass implementation of hydrogen as an energy carrier becomes commercially viable. This project aims to determine the kinetic and thermodynamic benefits of mesoporous metal scaffolds as reactive containment vessels for hydrogen storage materials. Fundamental experimental research into the synthesis, characterisation, and modification of nano-co ....Mesoporous Metal Scaffolds: Reactive Containment Vessels. The storage of hydrogen is one of the most important issues that remains to be solved before the mass implementation of hydrogen as an energy carrier becomes commercially viable. This project aims to determine the kinetic and thermodynamic benefits of mesoporous metal scaffolds as reactive containment vessels for hydrogen storage materials. Fundamental experimental research into the synthesis, characterisation, and modification of nano-confined hydrogen storage materials will be carried out. The results of this research are expected be used to tune hydrogen desorption temperatures and pressures of various light weight hydrogen storage materials to generate new materials attractive to the automobile industry.Read moreRead less
Benign recovery of precious metals from deep pristine environments. This project aims to extract precious metals from natural deposits conventional mining methods cannot reach. Glycine-peroxide systems can dissolve precious metals without pollution. Understanding these systems’ behaviour in natural orebodies could lead to in-situ leaching methods that complement conventional mining, especially in low grade deposits. This project intends to use a modern scientific workflow based on exploratory, d ....Benign recovery of precious metals from deep pristine environments. This project aims to extract precious metals from natural deposits conventional mining methods cannot reach. Glycine-peroxide systems can dissolve precious metals without pollution. Understanding these systems’ behaviour in natural orebodies could lead to in-situ leaching methods that complement conventional mining, especially in low grade deposits. This project intends to use a modern scientific workflow based on exploratory, descriptive and explanatory phases to model the coupled multi-physics of precious metals transport, introduce a high performance computing strategy for in-situ leaching, develop an experimental protocol that explains the recovery mechanisms, and propose optimal leaching patterns that maximise productivity.Read moreRead less
Exploring the gene regulation networks governing mitochondrial biogenesis in Arabidopsis. Mitochondria, subcellular organelles that perform many functions indispensable to plant growth and productivity, are dynamic compartments whose protein complement changes dramatically during plant development and under stress. Yet, the cellular processes that regulate the production of these organelles are virtually unknown. By combining conventional approaches with an extremely powerful holistic method for ....Exploring the gene regulation networks governing mitochondrial biogenesis in Arabidopsis. Mitochondria, subcellular organelles that perform many functions indispensable to plant growth and productivity, are dynamic compartments whose protein complement changes dramatically during plant development and under stress. Yet, the cellular processes that regulate the production of these organelles are virtually unknown. By combining conventional approaches with an extremely powerful holistic method for simultaneously examining the expression patterns of every gene in the model plant Arabidopsis, this project will identify proteins that regulate mitochondrial biosynthesis and uncover the gene networks that these proteins control. The project outcomes will provide new opportunities for the rational manipulation of plant growth and productivity.Read moreRead less
Electrostatic catalysis from single-molecule events to macroscopic systems. Electrostatics has important applications in day-to-day technologies, from recycling plastics to photocopying, but the exploration of how static charges affect chemical bonds and bonding is still in its infancy. This project aims to demonstrate the experimental links between the magnitude and polarity of an external electric field and chemical rates, expanding our understanding of chemical reactivity and transforming our ....Electrostatic catalysis from single-molecule events to macroscopic systems. Electrostatics has important applications in day-to-day technologies, from recycling plastics to photocopying, but the exploration of how static charges affect chemical bonds and bonding is still in its infancy. This project aims to demonstrate the experimental links between the magnitude and polarity of an external electric field and chemical rates, expanding our understanding of chemical reactivity and transforming our view of catalysis. By investigating the role of static electricity over systems selected from different sub-disciplines of chemistry, the project will derive the ground and selection rules for reactivity and selectivity by electrostatics. The project is expected to show that for chemical reactions of practical and conceptual value a specific catalyst can be replaced by a generic electric field stimulus, an invisible catalyst, enabling cleaner and cheaper opportunities that current technologies cannot fulfil.Read moreRead less