A novel DNA motif involved in plant mitochondrial stress responses. The future of Australia's agriculture is threatened by limited water resources, temperature extremes and soil salinity. This project aims to unravel how plants are able to adapt to this continuously changing environment, by focusing on the role of mitochondria - cellular compartments essential for energy metabolism and plant stress responses.
Functional analysis of novel mitochondrial outer membrane proteins in Arabidopsis. Mitochondria play central roles in the life and death of cells. This project will characterise the functions of proteins on the mitochondrial surface, which mediate signals that define mitochondrial function, providing novel approaches to modify mitochondrial function and plant growth.
Discovery Early Career Researcher Award - Grant ID: DE120101562
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Quantifying the contribution of leaf vein networks to the leaf economics spectrum in native and agricultural species. Using a combination of eco-physiological and geometric measures this project will evaluate the influence of leaf vein networks on leaf economics. It is expected that this work will identify vein investment and network design as major sources of variability underlying species adaptive strategies, and the global leaf economics spectrum as a whole.
Measuring protein turnover in vivo in plant mitochondria and chloroplasts to identify protease targets. This project plans to measure the rate at which proteins degrade inside plants by using stable isotopes of nitrogen and mass spectrometry analysis of isolated protein samples from different plant mutant lines. This will allow new insights into the in vivo role of specific proteases which are involved in regulating energy generating pathways in plant organelles and that are needed for light tol ....Measuring protein turnover in vivo in plant mitochondria and chloroplasts to identify protease targets. This project plans to measure the rate at which proteins degrade inside plants by using stable isotopes of nitrogen and mass spectrometry analysis of isolated protein samples from different plant mutant lines. This will allow new insights into the in vivo role of specific proteases which are involved in regulating energy generating pathways in plant organelles and that are needed for light tolerance, adaptation to day length, growth and normal leaf development. The new information will aid us to develop approaches to alter quality control of the plant proteome as a tool for engineering energy processes in plants.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150100130
Funder
Australian Research Council
Funding Amount
$362,000.00
Summary
Control of plant mitochondrial metabolism by reversible enzyme acetylation. Plant metabolism is more complex and less well understood than metabolism in other groups such as animals or bacteria. Our lack of understanding of how plants control their metabolism is currently a major roadblock in the development and use of plants to produce increased quantities of nutritional, medicinal and chemical compounds. It was recently discovered that animal and bacterial cells coordinate the activity of cent ....Control of plant mitochondrial metabolism by reversible enzyme acetylation. Plant metabolism is more complex and less well understood than metabolism in other groups such as animals or bacteria. Our lack of understanding of how plants control their metabolism is currently a major roadblock in the development and use of plants to produce increased quantities of nutritional, medicinal and chemical compounds. It was recently discovered that animal and bacterial cells coordinate the activity of central metabolic pathways via a specific chemical modification (acetylation) of key enzymes. As enzyme acetylation may function in plant cells as well, this project aims to perform a fundamental yet practical assessment of how this mechanism works in plants and how it can be exploited to accurately manipulate plant metabolism.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE120102913
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Dissecting proteolytic pathways that control chloroplast degradation and leaf senescence in Arabidopsis thaliana. Australian agriculture is threatened by worsening environmental conditions that cause premature ageing of plants leading to dramatic reductions in crop yields. This project aims to better understand plant senescence, thereby enabling the development of more robust and higher yielding crops.
Glutaredoxins (GRXs) as agents of redox homeostasis in mitochondria and respiratory-associated cell functions in plants. This project will test the importance of GRXs for the reduction/oxidation mediated network in plant mitochondria and moreover, uncover details of their dynamic features. This knowledge builds the basis for manipulation of mitochondrial GRXs in order to enhance the capability of the plant to cope with naturally occurring stresses.
Defence gene expression in Arabidopsis linked to metabolic perturbation and oxidative signalling via dsr1. Through analysis of a novel pathway of how plants perceive pathogens in the environment, this project aims to link metabolic energy generation pathways with pathogen defence. This could provide novel approaches for protect plants from pathogens by altering the sensitivity of this signalling pathway.
Dissecting novel roles of succinate dehydrogenase in stomatal aperture and root elongation in plants. Succinate dehydrogenase (complex II) is part of the respiration processes in plants and new evidence shows that reactive oxygen species generated by it can influence plant development and stress tolerance. However, there are still many unanswered questions about the composition and function of this enzyme and its dual roles in plants. This project will study this protein complex in the model p ....Dissecting novel roles of succinate dehydrogenase in stomatal aperture and root elongation in plants. Succinate dehydrogenase (complex II) is part of the respiration processes in plants and new evidence shows that reactive oxygen species generated by it can influence plant development and stress tolerance. However, there are still many unanswered questions about the composition and function of this enzyme and its dual roles in plants. This project will study this protein complex in the model plant Arabidopsis and the crop plant rice, identify its role in signalling in depth to provide knowledge adding development of strategies for improving tolerance of crops to stresses.Read moreRead less
The targeting of macromolecules to alter mitochondrial function. Mitochondria are essential organelles involved in energy production and specific metabolic pathways in plant cells that require the import of cytosolic transfer RNA (tRNA) to function. To date our knowledge on the mechanisms of tRNA import is limited. This project seeks to characterise putative receptors and mechanisms with the purpose of exploiting these insights to allow for the manipulation and modification of macromolecule targ ....The targeting of macromolecules to alter mitochondrial function. Mitochondria are essential organelles involved in energy production and specific metabolic pathways in plant cells that require the import of cytosolic transfer RNA (tRNA) to function. To date our knowledge on the mechanisms of tRNA import is limited. This project seeks to characterise putative receptors and mechanisms with the purpose of exploiting these insights to allow for the manipulation and modification of macromolecule targeting to mitochondria. The ability to modify or alter mitochondrial biogenesis and activity may allow for new approaches to be undertaken to increase plant growth, productivity and resistance to stress.Read moreRead less