The molecular pathogenesis of Equine rhinitis A virus, a major respiratory pathogen of horses. The equine industry in Australia is worth $15 billion a year. Equine respiratory disease costs Victoria, alone, $5-10 million per year. Equine rhinitis A virus is a major cause of acute febrile respiratory disease in horses. This project aims to develop an infectious clone to study the pathogenesis of the disease, and subunit antigens for use as vaccines. These reagents will increase our understand ....The molecular pathogenesis of Equine rhinitis A virus, a major respiratory pathogen of horses. The equine industry in Australia is worth $15 billion a year. Equine respiratory disease costs Victoria, alone, $5-10 million per year. Equine rhinitis A virus is a major cause of acute febrile respiratory disease in horses. This project aims to develop an infectious clone to study the pathogenesis of the disease, and subunit antigens for use as vaccines. These reagents will increase our understanding of the pathogenesis of ERAV and will lead to experimental vaccines which will be tested in horses.Read moreRead less
Development of an anti-Chlamydia vaccine for the koala. The koala is one of Australia's main icons and a major drawcard for tourists. However, it suffers from debilitating disease due to the bacterium Chlamydia, which can lead to severe conjunctivitis, eventual blindness in both sexes, and the females develop untreatable cysts and can become infertile. This project will develop a Chlamydia vaccine to be administered to healthy and diseased koalas in zoos, sanctuaries, koala care centres, relocat ....Development of an anti-Chlamydia vaccine for the koala. The koala is one of Australia's main icons and a major drawcard for tourists. However, it suffers from debilitating disease due to the bacterium Chlamydia, which can lead to severe conjunctivitis, eventual blindness in both sexes, and the females develop untreatable cysts and can become infertile. This project will develop a Chlamydia vaccine to be administered to healthy and diseased koalas in zoos, sanctuaries, koala care centres, relocation programs and eventually perhaps even wild populations. The vaccine findings may also be transferable to other animals and may also even assist the development of a human Chlamydia vaccine.Read moreRead less
A comprehensive analysis of the outer membrane, surface exposed and secreted proteome of Pasteurella multocida. Pasteurella multocida is the causative agent of a range of animal diseases. The molecular mechanisms of P. multocida pathogenesis are poorly understood and the current vaccines generally ineffective. We will identify all P. multocida outer membrane, surface exposed and secreted proteins expressed during natural infection, or under conditions which mimic natural infection, by a global p ....A comprehensive analysis of the outer membrane, surface exposed and secreted proteome of Pasteurella multocida. Pasteurella multocida is the causative agent of a range of animal diseases. The molecular mechanisms of P. multocida pathogenesis are poorly understood and the current vaccines generally ineffective. We will identify all P. multocida outer membrane, surface exposed and secreted proteins expressed during natural infection, or under conditions which mimic natural infection, by a global proteomics approach. We believe that secreted proteins and those found on the outer surface of the bacterial cell are likely to be crucial virulence determinants. The expected outcomes are the identification of a number of candidate vaccine antigens and an enhanced understanding of Pasteurella pathogenesis.Read moreRead less
Molecular and antibody analysis of cytomegalovirus (CMV) infection of fetal and placental cells. CMV is a beta herpesvirus with many unknown molecular mechanisms associated with cellular infection. The virus infects placental cells in vivo, although pathogenesis of viral damage to these cells has been extremely difficult to study in vitro. We have commenced a study to i) demonstrate the molecular accompaniments of infection of placental cells in vitro, ii) determine the genotypic characteristics ....Molecular and antibody analysis of cytomegalovirus (CMV) infection of fetal and placental cells. CMV is a beta herpesvirus with many unknown molecular mechanisms associated with cellular infection. The virus infects placental cells in vivo, although pathogenesis of viral damage to these cells has been extremely difficult to study in vitro. We have commenced a study to i) demonstrate the molecular accompaniments of infection of placental cells in vitro, ii) determine the genotypic characteristics of congenital CMV infections, in collaboration with Abbott Diagnostics, and iii) produce an in vivo model of CMV infection to demonstrate the pathogenesis of cellular injury. The combination of molecular expertise at UNSW with monoclonal antibody expertise from Abbott Diagnostics mean this project is unique worldwide.Read moreRead less
Recombinant probiotics for prevention of enteric infections in piglets. Shigatoxigenic and enterotoxigenic strains of Escherichia coli cause severe diarrhoeal disease and oedema disease in piglets. The resultant morbidity and mortality is a major cause of financial loss to the pig industry both in Australia and overseas. We have conceived a highly novel approach to prevention of these diseases involving molecular mimicry of host receptors for the E. coli toxins and adhesins on the surface of a ....Recombinant probiotics for prevention of enteric infections in piglets. Shigatoxigenic and enterotoxigenic strains of Escherichia coli cause severe diarrhoeal disease and oedema disease in piglets. The resultant morbidity and mortality is a major cause of financial loss to the pig industry both in Australia and overseas. We have conceived a highly novel approach to prevention of these diseases involving molecular mimicry of host receptors for the E. coli toxins and adhesins on the surface of a harmless bacterium. Oral administration of this agent to piglets has the potential to bind free toxins in the gut and prevent colonization of the intestines by the pathogens, thereby preventing disease.Read moreRead less
Functional genomics of large clostridial plasmids. The aims of this genomics project are to determine how large DNA elements called plasmids are able to be transferred between different strains of a bacterium that causes disease in domestic livestock. These plasmids carry genes that encode the potent protein toxins that are responsible for several diseases. To understand how these diseases are spread we must learn how the plasmids have evolved and whether they can move from bacterium to bacteriu ....Functional genomics of large clostridial plasmids. The aims of this genomics project are to determine how large DNA elements called plasmids are able to be transferred between different strains of a bacterium that causes disease in domestic livestock. These plasmids carry genes that encode the potent protein toxins that are responsible for several diseases. To understand how these diseases are spread we must learn how the plasmids have evolved and whether they can move from bacterium to bacterium. The successful completion of the project will result in a detailed understanding of genetic elements that are important mediators of several diseases of importance to Australian primary industry.Read moreRead less
Characterising post-translational modifications in bacterial proteins. This project represents the first global attempt to characterize post-translational modifications in bacterial proteins using the tools of proteomics. Modifications to proteins are key elements in altering their function. In bacteria, modifications are important in cell-cell adhesion, signalling and triggering of the immune response. Characterisation of modified proteins and their sites of modification represents an opportuni ....Characterising post-translational modifications in bacterial proteins. This project represents the first global attempt to characterize post-translational modifications in bacterial proteins using the tools of proteomics. Modifications to proteins are key elements in altering their function. In bacteria, modifications are important in cell-cell adhesion, signalling and triggering of the immune response. Characterisation of modified proteins and their sites of modification represents an opportunity to understand how bacterial cell populations communicate in the environment, as well as aid in understanding pathogenesis in medical, veterinary and food-borne pathogens. Therefore, improved vaccine targets and therapeutics, as well as method-based products, may be generated by this project.Read moreRead less
Pathogenesis, regulation and genomics of the ovine footrot pathogen, Dichelobacter nodosus. Footrot is one of the most economically significant diseases of sheep in Australia. The aim of this project is to develop a detailed understanding of how the bacterium that causes this infection is able to infect the sheep hoof and result in clinical disease. The complete sequence of the genome of the causative bacterium will be determined, enabling us to deduce its genetic potential. The completed projec ....Pathogenesis, regulation and genomics of the ovine footrot pathogen, Dichelobacter nodosus. Footrot is one of the most economically significant diseases of sheep in Australia. The aim of this project is to develop a detailed understanding of how the bacterium that causes this infection is able to infect the sheep hoof and result in clinical disease. The complete sequence of the genome of the causative bacterium will be determined, enabling us to deduce its genetic potential. The completed project will significantly advance fundamental knowledge of the disease process and will lead to the development of improved methods for the control of the disease, with concomitant cost savings to Australian primary industry.Read moreRead less
Proteomics and vaccine development in swine dysentery. Swine dysentery is an infectious disease of significant economic importance caused by Brachyspira hyodysenteriae. There is no effective vaccine available. This project will combine modern techniques in microbial genomics and proteomics to identify outer membrane proteins of B. hyodysenteriae and evaluate their role as candidate vaccine antigens.
Molecular Cell Biology and Comparative Genomics Of Planctomycetes and Verrucomicrobia In Relation To Evolution Of Cytoskeletal Proteins and Membrane-bounded Compartments. Planctomycetes and verrucomicrobia are evolutionarily distinct groups of bacteria which possess unusual cell structure and which share some significant genes important in cell biology with eukaryotes e.g. in verrucomicrobia the cytoskeleton protein tubulin. These bacteria are important for understanding the transition from no ....Molecular Cell Biology and Comparative Genomics Of Planctomycetes and Verrucomicrobia In Relation To Evolution Of Cytoskeletal Proteins and Membrane-bounded Compartments. Planctomycetes and verrucomicrobia are evolutionarily distinct groups of bacteria which possess unusual cell structure and which share some significant genes important in cell biology with eukaryotes e.g. in verrucomicrobia the cytoskeleton protein tubulin. These bacteria are important for understanding the transition from non-nucleated cells with simple cell division to nucleated cells with chromosome separation via cytoskeletal protein movement.The project will compare genomes of of planctomycetes and verrucomicrobia to determine their relationship, determine whether the tubulin homolog of verrucomicrobia can form cytoskeleton structures, and characterize the cytoskeleton of ammonium-oxidizing planctomycetes used in wastewater treatment.Read moreRead less