Dynamics of constrained Brownian motion of neuro-secretory vesicles. This project will shed light on a fundamental problem the mechanism of brain cell communication by use of quantitative biophotonics methods including laser tracking, optical tweezers and three dimensional fluorescence microscopy. This work will give valuable new clues to finally solve the dynamics of molecular interactions underpinning neuronal communication.
Force microscopy with arbitrary optically-trapped probes and application to internal mechanics of cells. The ability to perform micromanipulation on particles, macromolecules, subcellular organelles, or whole cells is fundamental in elucidating processes such as chromosome movement during cell division, and movement of cell components in and out of the cell. The recent advances in optical tweezers have allowed this type of micromanipulation to approach reality. However, determination of the true ....Force microscopy with arbitrary optically-trapped probes and application to internal mechanics of cells. The ability to perform micromanipulation on particles, macromolecules, subcellular organelles, or whole cells is fundamental in elucidating processes such as chromosome movement during cell division, and movement of cell components in and out of the cell. The recent advances in optical tweezers have allowed this type of micromanipulation to approach reality. However, determination of the true optical force is critical for this technique to reach its full potential. This project will develop novel techniques to quantitatively determine the absolute optical force applied to the cell component using the process of ingestion (phagocytosis) as a proof-of-principle test, and measure forces in chromosome movement and vesicle transport within cells.Read moreRead less