Mitochondrial Biogenesis and Signalling in Plants . This proposal aims to define the mechanisms of how mitochondrial growth and stress signalling interact and are regulated. Mitochondria are central machines in cells that use energy obtained through photosynthesis to drive growth and also play an important role in sensing and responding to non-optimal environmental growth conditions. As mitochondrial growth and stress signalling are antagonistic, growth is retarded when stress signalling is acti ....Mitochondrial Biogenesis and Signalling in Plants . This proposal aims to define the mechanisms of how mitochondrial growth and stress signalling interact and are regulated. Mitochondria are central machines in cells that use energy obtained through photosynthesis to drive growth and also play an important role in sensing and responding to non-optimal environmental growth conditions. As mitochondrial growth and stress signalling are antagonistic, growth is retarded when stress signalling is activated. Thus, the outcomes will be new knowledge and understanding of how plants balance growth and stress responses. This benefit of this knowledge and understanding is that it can be used to pursue novel avenues to optimise crop performance in changing and adverse environments.Read moreRead less
Improving grain legume seeds for future climates. Grain legumes are essential for sustainable agriculture and human dietary protein, but seed quality is predicted to decline under future scenarios of high CO2 and warmer temperatures. This project aims to improve legume seed quality under future climates by comparing metabolites and physiological traits of chickpea and other legumes to establish mechanisms by which legumes maximise seed nutrient allocation. The anticipated outcomes include new me ....Improving grain legume seeds for future climates. Grain legumes are essential for sustainable agriculture and human dietary protein, but seed quality is predicted to decline under future scenarios of high CO2 and warmer temperatures. This project aims to improve legume seed quality under future climates by comparing metabolites and physiological traits of chickpea and other legumes to establish mechanisms by which legumes maximise seed nutrient allocation. The anticipated outcomes include new metabolite-based breeding markers for the improvement of crops with higher seed proteins, micronutrients and bioactive compounds that are adapted to future climates. Seed nutrient improvement will also include increased biological nitrogen fixation to reduce the need for chemical nitrogen fertilisers.Read moreRead less
Australia's native sorghums: a model for testing plant adaptation theories. This proposal tests an emerging theory that allocation of resources by plants to growth or defence are interrelated, not alternatives as currently assumed. Like many crops, sorghum produces toxic cyanide, especially during droughts but its wild relatives make much less. This project aims to discover why cyanide is so common in domesticated plants and why levels increase with stress. This has important implications for de ....Australia's native sorghums: a model for testing plant adaptation theories. This proposal tests an emerging theory that allocation of resources by plants to growth or defence are interrelated, not alternatives as currently assumed. Like many crops, sorghum produces toxic cyanide, especially during droughts but its wild relatives make much less. This project aims to discover why cyanide is so common in domesticated plants and why levels increase with stress. This has important implications for developing crops that are high yielding and also climate resilient. Expected outcomes include full genome sequences for all of Australia’s unique native sorghums, confirmation of new theories on the interrelationships between defence and growth and identification of new traits vital for developing the crops of the future. Read moreRead less
Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We ....Deciphering organelle transport mechanisms in plants. Plant growth, productivity and seed yield all depend on organelle function which requires metabolites and proteins
to be transported across membranes. This mechanism of transport is carried out by specific transporters that have
the ability to transport macromolecules, and regulate organelle function. We have identified new transporters that
are involved in amino acid and protein transport in the mitochondria, chloroplast and peroxisomes. We will assign
function to each protein and investigate the importance in regulating organelle biogenesis. This will allow us to
modulate plant energy production for optimal growth and to withstand abiotic stress, all of which have
agriculturally beneficial consequences. Read moreRead less
Boosting C4 photosynthesis to climate proof crop yields. Building next generation C4 crops, such as maize, sugarcane and sorghum, to cope with drought and heat stress is requisite to ensure the supply of food and fodder. Here we will increase the content and / or catalytic efficiency of the primary carboxylase of C4 photosynthesis (PEPC) that supplies CO2 to the carbon concentrating mechanism and ensures high photosynthetic rates. We will develop new SynBio tools to create and test novel PEPC is ....Boosting C4 photosynthesis to climate proof crop yields. Building next generation C4 crops, such as maize, sugarcane and sorghum, to cope with drought and heat stress is requisite to ensure the supply of food and fodder. Here we will increase the content and / or catalytic efficiency of the primary carboxylase of C4 photosynthesis (PEPC) that supplies CO2 to the carbon concentrating mechanism and ensures high photosynthetic rates. We will develop new SynBio tools to create and test novel PEPC isoforms with desirable properties. Ultimately, the project aims to identify isoforms that improve plant fitness under stress conditions. Optimising PEPC activity will provide next generation solutions to improve water balance and carbon assimilation to keep C4 crops productive under future climates.Read moreRead less
Tree-mediated methane fluxes: A new frontier in the global carbon cycle. Methane is an extremely potent greenhouse gas. Recent evidence suggests that tree-mediated fluxes may be a significant, but overlooked source of methane to the atmosphere. This project aims to quantify the magnitude and drivers of tree-mediated methane fluxes from Australia’s dominant forest types. Innovatively, we will be using a novel combination of empirical field based measurements, gas tracer experiments, microbial ana ....Tree-mediated methane fluxes: A new frontier in the global carbon cycle. Methane is an extremely potent greenhouse gas. Recent evidence suggests that tree-mediated fluxes may be a significant, but overlooked source of methane to the atmosphere. This project aims to quantify the magnitude and drivers of tree-mediated methane fluxes from Australia’s dominant forest types. Innovatively, we will be using a novel combination of empirical field based measurements, gas tracer experiments, microbial analysis and modelling methods. Expected outcomes are a mechanistic understanding of tree-mediated methane fluxes, helping to constrain regional, national and global methane budgets. The results of this study will help inform publicly funded greenhouse gas abatement strategies, ensuring a maximal return on investment.Read moreRead less
Connecting soil nitrogen and plant uptake for greener agriculture. This project will use synthetic organic chemistry, biochemistry, root and rhizosphere biology and rhizosphere modelling to establish detailed mechanistic knowledge of the nitrogen (N) transport and uptake processes at the soil-root interface to develop new, efficient urease and nitrification inhibitors for reliable provision of N to the plant/root system. The reduction of excessive N fertilisation has significant environmental be ....Connecting soil nitrogen and plant uptake for greener agriculture. This project will use synthetic organic chemistry, biochemistry, root and rhizosphere biology and rhizosphere modelling to establish detailed mechanistic knowledge of the nitrogen (N) transport and uptake processes at the soil-root interface to develop new, efficient urease and nitrification inhibitors for reliable provision of N to the plant/root system. The reduction of excessive N fertilisation has significant environmental benefits by reducing greenhouse gas emissions and water pollution. This project will lead to a breakthrough for the triple challenge of food security, environmental degradation and climate change, while improving plant productivity and increasing the profitability of agriculture through lower fertiliser costs.Read moreRead less
Seaweed forests of the future: responses to ocean acidification and warming. The aim is to discover if rising levels of oceanic carbon dioxide will offset negative effects of ocean warming on seaweeds, using targeted physiological experiments together with novel molecular diagnostics. Seaweeds create habitats and food for shellfish and fish, and play a crucial role in long term ‘blue carbon’ storage. They are predicted to benefit from future carbon dioxide enrichment, but to test this forecast r ....Seaweed forests of the future: responses to ocean acidification and warming. The aim is to discover if rising levels of oceanic carbon dioxide will offset negative effects of ocean warming on seaweeds, using targeted physiological experiments together with novel molecular diagnostics. Seaweeds create habitats and food for shellfish and fish, and play a crucial role in long term ‘blue carbon’ storage. They are predicted to benefit from future carbon dioxide enrichment, but to test this forecast requires a detailed understanding of the mechanisms used by seaweeds to acquire dissolved inorganic carbon. The expected outcome is robust predictions of how the primary productivity of coastal waters will respond to future high carbon dioxide conditions, enabling human adaptation to environmental change.
Read moreRead less
Evolution and specificity of alternative splicing in plants. This project aims to elucidate fundamental principles of alternative splicing, a basic mechanism that plays a vital role in several biological processes across all organisms. Plants are highly effective in adapting to varied environmental, seasonal and climatic conditions and this project aims to uncover how alternative splicing contributes to regulation of gene expression in response to developmental and environmental cues. Uncovering ....Evolution and specificity of alternative splicing in plants. This project aims to elucidate fundamental principles of alternative splicing, a basic mechanism that plays a vital role in several biological processes across all organisms. Plants are highly effective in adapting to varied environmental, seasonal and climatic conditions and this project aims to uncover how alternative splicing contributes to regulation of gene expression in response to developmental and environmental cues. Uncovering the underlying mechanisms of alternative splicing will not only advance fundamental knowledge, but also has the potential to provide tools and technologies through which sensitivities of plants to environmental stress can be potentially manipulated to benefit agriculture.Read moreRead less
How is the plant genome reactivated and controlled during seed germination? This project aims to determine the mechanisms by which plant genomes are regulated during seed germination. The genomes of cells in mature, inactive seeds are repressed, but later must be rapidly reactivated to allow the gene expression that drives early seedling growth and development. This project will study proteins that turn genes on and off, and how these interact with the structure of DNA, in order to understand ho ....How is the plant genome reactivated and controlled during seed germination? This project aims to determine the mechanisms by which plant genomes are regulated during seed germination. The genomes of cells in mature, inactive seeds are repressed, but later must be rapidly reactivated to allow the gene expression that drives early seedling growth and development. This project will study proteins that turn genes on and off, and how these interact with the structure of DNA, in order to understand how spatial and temporal patterns of gene expression are controlled. It will advance our understanding of genome regulatory programs controlling germination and growth, and how they vary between Arabidopsis and barley. This can improve our ability to manipulate seed behaviour which would benefit growers and producers. Read moreRead less