Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We ....Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We will generate antibody fragments that bind these MP and express them in tomato. We will analyse antibody expression in tomato and study their association with the phytoplasma membrane. Transgenic plants will be inoculated with phytoplasma and monitored for resistance. This work represents a major step towards engineering resistance to phytoplasmas.Read moreRead less
Water-use efficiency of Australian tropical trees: mechanistic analysis at multiple scales. The proposed research will provide valuable information about the physiological functioning of trees in northern Australia. Experiments will elucidate mechanisms that can result in variation in water-use efficiency among different tree species. Such a mechanistic understanding will have multiple benefits: (1) results will be able to be incorporated into process-based models of carbon and water cycling ....Water-use efficiency of Australian tropical trees: mechanistic analysis at multiple scales. The proposed research will provide valuable information about the physiological functioning of trees in northern Australia. Experiments will elucidate mechanisms that can result in variation in water-use efficiency among different tree species. Such a mechanistic understanding will have multiple benefits: (1) results will be able to be incorporated into process-based models of carbon and water cycling in the north-Australian landscape; (2) they will provide valuable information for land managers interested in optimizing both plant biomass production and water resource management; and (3) they will provide a critical test of proxy methods for identifying high water-use efficiency in taxonomically diverse tree species.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100015
Funder
Australian Research Council
Funding Amount
$500,000.00
Summary
An Ultra High Resolution Mass Spectrometry Facility for Lipidomics Research. An ultra-high-resolution mass spectrometry facility for lipidomics research:
This proposal aims to establish an ultra-high-resolution, accurate mass spectrometry facility in Australia for comprehensive lipidomics research. The platform would consist of a Thermo Scientific Orbitrap Fusion mass spectrometer interfaced with ultra-high-pressure high-performance liquid chromatography. This proposal will address a major need ....An Ultra High Resolution Mass Spectrometry Facility for Lipidomics Research. An ultra-high-resolution mass spectrometry facility for lipidomics research:
This proposal aims to establish an ultra-high-resolution, accurate mass spectrometry facility in Australia for comprehensive lipidomics research. The platform would consist of a Thermo Scientific Orbitrap Fusion mass spectrometer interfaced with ultra-high-pressure high-performance liquid chromatography. This proposal will address a major need for advanced mass spectrometry-based lipid analysis capabilities across mammalian, plant, parasite, and microalgae research disciplines, as well as enabling fundamental studies of lipid separation, chemistry and reactivity. The instrumentation would be applicable to a diverse range of projects including studies of the role of lipid metabolism in mammalian biochemistry and cell biology, plant biology and parasitology, and micro algae biofuel production.Read moreRead less